Cloned (Comment) | Organism |
---|---|
expresssion of the mitochondrial isozyme in Escherichia coli BL21(DE3) | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics with diverse mutant variants of tRNASerUGA and tRNASerGCU with the recombinant mitochondrial isozyme, overview | Bos taurus | |
0.22 | - |
tRNASerUGA | native wild-type enzyme, pH 8.5, 37°C | Bos taurus | |
0.29 | - |
tRNASerUGA | recombinant wild-type enzyme, pH 8.5, 37°C | Bos taurus | |
0.35 | - |
tRNASerGCU | recombinant wild-type enzyme, pH 8.5, 37°C | Bos taurus | |
0.37 | - |
tRNASerGCU | native wild-type enzyme, pH 8.5, 37°C | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Bos taurus | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
2 * 57000, recombinant mitochondrial isozyme, SDS-PAGE | Bos taurus |
109000 | - |
recombinant mitochondrial isozyme, gel filtration | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-serine + tRNASer | Bos taurus | - |
AMP + diphosphate + L-seryl-tRNASer | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | Q9N0F3 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + L-serine + tRNASer = AMP + diphosphate + L-seryl-tRNASer | tRNA recoginition mechanism, dual recognition mode for isoacceptors, the mitochondrial isozyme specifically recognizes the TPiC loop sequence in each isoacceptor | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-serine + tRNASer | - |
Bos taurus | AMP + diphosphate + L-seryl-tRNASer | - |
? | |
ATP + L-serine + tRNASerGCU | GCU for codon AGY, unusual tRNASer substrate, the mitochondrial isozyme aminoacylates both mitochondrial tRNASer types, the first corresponding to the codon AGY lacking the entire D arm | Bos taurus | AMP + diphosphate + L-seryl-tRNASerGCU | - |
? | |
ATP + L-serine + tRNASerUGA | UGA for codon UCN, unusual tRNASer substrate, the mitochondrial isozyme aminoacylates both mitochondrial tRNASer types, the second corresponding to the codon UCN with a different cloverleaf structure | Bos taurus | AMP + diphosphate + L-seryl-tRNASerUGA | - |
? | |
additional information | substrate specificity with diverse mutant variants of tRNASerUGA and tRNASerGCU with the recombinant mitochondrial isozyme, overview | Bos taurus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 57000, recombinant mitochondrial isozyme, SDS-PAGE | Bos taurus |
Synonyms | Comment | Organism |
---|---|---|
62 kDa RNA-binding protein | - |
Bos taurus |
Serine transfer RNA synthetase | - |
Bos taurus |
Serine translase | - |
Bos taurus |
Serine--tRNA ligase | - |
Bos taurus |
SerRS | - |
Bos taurus |
SerRSmt | - |
Bos taurus |
SERSEC | - |
Bos taurus |
Seryl-transfer ribonucleate synthetase | - |
Bos taurus |
Seryl-transfer ribonucleic acid synthetase | - |
Bos taurus |
Seryl-transfer RNA synthetase | - |
Bos taurus |
Seryl-tRNA synthetase | - |
Bos taurus |
Synthetase, seryl-transfer ribonucleate | - |
Bos taurus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kcat for diverse mutant variants of tRNASerUGA and tRNASerGCU with the recombinant mitochondrial isozyme, overview | Bos taurus | |
0.35 | - |
tRNASerGCU | native wild-type enzyme, pH 8.5, 37°C | Bos taurus | |
0.63 | - |
tRNASerUGA | native wild-type enzyme, pH 8.5, 37°C | Bos taurus | |
0.64 | - |
tRNASerGCU | recombinant wild-type enzyme, pH 8.5, 37°C | Bos taurus | |
0.67 | - |
tRNASerUGA | recombinant wild-type enzyme, pH 8.5, 37°C | Bos taurus | |
6.08 | - |
tRNASerUGA | native wild-type enzyme, pH 8.5, 37°C | Bos taurus | |
6.08 | - |
tRNASerUGA | recombinant wild-type enzyme, pH 8.5, 37°C | Bos taurus | |
6.08 | - |
tRNASerGCU | recombinant wild-type enzyme, pH 8.5, 37°C | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Bos taurus |