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Literature summary for 6.1.1.10 extracted from
- Methionyl-tRNA synthetase from Phaseolus aureus. Purification and properties (1981), Plant Physiol., 67, 325-329.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ethionine | methionyl-tRNA formation | Vigna radiata var. radiata |  |
| methionine | selenomethionyl-tRNA formation | Vigna radiata var. radiata | |
| selenomethionine | methionyl-tRNA formation | Vigna radiata var. radiata | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.032 | - |
Met | methionine-dependent ATP-diphosphate exchange | Vigna radiata var. radiata | |
| 0.038 | - |
selenomethionine | selenomethionine-dependent ATP-diphosphate exchange | Vigna radiata var. radiata | |
| 1.3 | - |
ATP | ATP-diphosphate exchange | Vigna radiata var. radiata | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | absolutely dependent on | Vigna radiata var. radiata | |
| Mg2+ | optimal Mg2+/ATP ratio: 2.5 | Vigna radiata var. radiata | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 85000 | - |
4 * 85000, SDS-PAGE | Vigna radiata var. radiata |
| 340000 | - |
gel filtration | Vigna radiata var. radiata |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vigna radiata var. radiata | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Vigna radiata var. radiata |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | - |
Vigna radiata var. radiata | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.161 | - |
- |
Vigna radiata var. radiata |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-methionine + tRNAMet | - |
Vigna radiata var. radiata | AMP + diphosphate + L-methionyl-tRNAMet | - |
? | |
| ATP + selenomethionine + tRNAMet | - |
Vigna radiata var. radiata | AMP + diphosphate + selenomethionyl-tRNAMet | - |
? | |
| additional information | also catalyzes selenomethionine- and selenoethionine-dependent ATP-diphosphate exchange | Vigna radiata var. radiata | ? | - |
? | |
| additional information | no other amino acid than methionine is activated in ATP-diphosphate exchange | Vigna radiata var. radiata | ? | - |
? | |
| additional information | methionine-dependent ATP-diphosphate exchange | Vigna radiata var. radiata | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 85000, SDS-PAGE | Vigna radiata var. radiata |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | 8.3 | - |
Vigna radiata var. radiata |
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Release 2023.1 (January 2023)
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