Application | Comment | Organism |
---|---|---|
biotechnology | site-specific incorporation of 3-iodo-L-tyrosine into proteins in a cell-free system for use in specialized in vitro translation systems | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
overexpression of His-tagged wild-type and mutants in strain BL21(DE3) | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
Q179A | site-directed mutagenesis, reduced activity with L-tyrosine, no activity with 3-iodo-L-tyrosine | Escherichia coli |
Q179E | inactive mutant | Escherichia coli |
Q179N | site-directed mutagenesis, reduced activity with L-tyrosine, no activity with 3-iodo-L-tyrosine | Escherichia coli |
Q179S | site-directed mutagenesis, reduced activity with L-tyrosine, no activity with 3-iodo-L-tyrosine | Escherichia coli |
Q179Y | inactive mutant | Escherichia coli |
Q195A | site-directed mutagenesis, active with L-tyrosine, low activity with 3-iodo-L-tyrosine | Escherichia coli |
Q195C | site-directed mutagenesis, reduced activity with L-tyrosine, low activity with 3-iodo-L-tyrosine | Escherichia coli |
Q195D | site-directed mutagenesis, highly reduced activity with L-tyrosine, low activity with 3-iodo-L-tyrosine | Escherichia coli |
Q195E | site-directed mutagenesis, active with L-tyrosine, no activity with 3-iodo-L-tyrosine | Escherichia coli |
Q195F | inactive mutant | Escherichia coli |
Q195G | site-directed mutagenesis, reduced activity with L-tyrosine, no activity with 3-iodo-L-tyrosine | Escherichia coli |
Q195H | site-directed mutagenesis, active with L-tyrosine, no activity with 3-iodo-L-tyrosine | Escherichia coli |
Q195I | site-directed mutagenesis, highly reduced activity with L-tyrosine, no activity with 3-iodo-L-tyrosine | Escherichia coli |
Q195K | inactive mutant | Escherichia coli |
Q195L | site-directed mutagenesis, reduced activity with L-tyrosine, no activity with 3-iodo-L-tyrosine | Escherichia coli |
Q195M | site-directed mutagenesis, highly reduced activity with L-tyrosine, no activity with 3-iodo-L-tyrosine | Escherichia coli |
Q195N | site-directed mutagenesis, reduced activity with L-tyrosine, low activity with 3-iodo-L-tyrosine | Escherichia coli |
Q195R | inactive mutant | Escherichia coli |
Q195S | site-directed mutagenesis, active with L-tyrosine, low activity with 3-iodo-L-tyrosine | Escherichia coli |
Q195T | site-directed mutagenesis, active with L-tyrosine, no activity with 3-iodo-L-tyrosine | Escherichia coli |
Q195V | site-directed mutagenesis, reduced activity with L-tyrosine, no activity with 3-iodo-L-tyrosine | Escherichia coli |
Q195W | inactive mutant | Escherichia coli |
Q195Y | site-directed mutagenesis, reduced activity with L-tyrosine, no activity with 3-iodo-L-tyrosine | Escherichia coli |
Y37A/Q195A | inactive mutant | Escherichia coli |
Y37A/Q195C | site-directed mutagenesis, active with L-tyrosine and 3-iodo-L-tyrosine, preference for the latter, reduced overall activity | Escherichia coli |
Y37A/Q195N | inactive mutant | Escherichia coli |
Y37A/Q195S | site-directed mutagenesis, equally low activity with L-tyrosine and 3-iodo-L-tyrosine | Escherichia coli |
Y37I/Q195A | site-directed mutagenesis, equally low activity with L-tyrosine and 3-iodo-L-tyrosine | Escherichia coli |
Y37I/Q195C | inactive mutant | Escherichia coli |
Y37I/Q195N | inactive mutant | Escherichia coli |
Y37I/Q195S | inactive mutant | Escherichia coli |
Y37L/Q195A | site-directed mutagenesis, highly reduced activity with L-tyrosine, no activity with 3-iodo-L-tyrosine | Escherichia coli |
Y37L/Q195C | site-directed mutagenesis, equally low activity with L-tyrosine and 3-iodo-L-tyrosine | Escherichia coli |
Y37L/Q195N | site-directed mutagenesis, highly reduced activity with L-tyrosine, no activity with 3-iodo-L-tyrosine | Escherichia coli |
Y37L/Q195S | inactive mutant | Escherichia coli |
Y37V/Q195N | site-directed mutagenesis, active with L-tyrosine and 3-iodo-L-tyrosine, preference for the latter, reduced overall activity | Escherichia coli |
Y37V/Q195S | site-directed mutagenesis, reduced activity with L-tyrosine and 3-iodo-L-tyrosine | Escherichia coli |
Y73A | site-directed mutagenesis, equally active with L-tyrosine and 3-iodo-L-tyrosine | Escherichia coli |
Y73F | site-directed mutagenesis, active with L-tyrosine, no activity with 3-iodo-L-tyrosine | Escherichia coli |
Y73G | site-directed mutagenesis, equally active with L-tyrosine and 3-iodo-L-tyrosine, reduced overall activity | Escherichia coli |
Y73H | site-directed mutagenesis, active with L-tyrosine, and slightly active with 3-iodo-L-tyrosine | Escherichia coli |
Y73I | site-directed mutagenesis, active with L-tyrosine and 3-iodo-L-tyrosine, preference for the latter | Escherichia coli |
Y73L | site-directed mutagenesis, equally active with L-tyrosine and 3-iodo-L-tyrosine | Escherichia coli |
Y73M | site-directed mutagenesis, equally active with L-tyrosine and 3-iodo-L-tyrosine | Escherichia coli |
Y73S | site-directed mutagenesis, active with L-tyrosine and 3-iodo-L-tyrosine, preference for the first | Escherichia coli |
Y73V | site-directed mutagenesis, equally active with L-tyrosine and 3-iodo-L-tyrosine | Escherichia coli |
Y73V/Q195A | site-directed mutagenesis, active with L-tyrosine and 3-iodo-L-tyrosine, preference for the latter, reduced overall activity | Escherichia coli |
Y73V/Q195C | site-directed mutagenesis, 10fold more active with 3-iodo-L-tyrosine than with L-tyrosine, reduced overall activity | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0053 | - |
L-tyrosine | pH 7.6, 37°C, wild-type enzyme | Escherichia coli | |
0.13 | - |
3-iodo-L-tyrosine | pH 7.6, 37°C, mutant Y73V/Q195C | Escherichia coli | |
0.14 | - |
L-tyrosine | pH 7.6, 37°C, mutant Y73V/Q195C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-tyrosine + tRNATyr | Escherichia coli | - |
AMP + L-Tyr-tRNATyr + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant enzymes from strain BL21(DE3) | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + L-tyrosine + tRNATyr = AMP + diphosphate + L-tyrosyl-tRNATyr | amino acid residues Y37 and Q195 are involved in substrate specificity determination | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3-iodo-L-tyrosine + tRNATyr | mutant Y73V/Q195C and other mutants, no activity with the wild-type enzyme | Escherichia coli | AMP + L-Tyr-tRNATyr + diphosphate | - |
? | |
ATP + L-tyrosine + tRNATyr | - |
Escherichia coli | AMP + L-Tyr-tRNATyr + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Tyrosyl-tRNA synthetase | - |
Escherichia coli |
TyrRS | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.045 | - |
L-tyrosine | pH 7.6, 37°C, mutant Y73V/Q195C | Escherichia coli | |
0.43 | - |
3-iodo-L-tyrosine | pH 7.6, 37°C, mutant Y73V/Q195C | Escherichia coli | |
12 | - |
L-tyrosine | pH 7.6, 37°C, wild-type enzyme | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |