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Literature summary for 5.6.2.2 extracted from

  • Skladanowski, A.; Come, M.G.; Sabisz, M.; Escargueil, A.E.; Larsen, A.K.
    Down-regulation of DNA topoisomerase IIalpha leads to prolonged cell cycle transit in G2 and early M phases and increased survival to microtubule-interacting agents (2005), Mol. Pharmacol., 68, 625-634.
    View publication on PubMed
Search for more literature for 5.6.2.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in DC-3F cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
amsacrine reduced inhibition of the enzyme in vincristine-resistant cells Homo sapiens
ICRF-187 reduced inhibition of the enzyme in vincristine-resistant cells Homo sapiens
Mitoxantrone reduced inhibition of the enzyme in vincristine-resistant cells Homo sapiens
additional information vincristine selection and resistance is accompanied by specific downregulation of isozyme IIalpha, but of isozyme IIbeta, leading to at least 7fold reduced chromosome-associated isozyme IIalpha Homo sapiens
Symadex i.e. C-1311, reduced inhibition of the enzyme in vincristine-resistant cells Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
 Homo sapiens 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
KCl required for decatenation activity Homo sapiens
Mg2+ required for decatenation activity Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens the amount of isozyme IIalpha in cells influences directly the cell cycle kinetics in G2 and early mitosis as well as the resistance to nocodazole ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 isozymes IIalpha and IIbeta
-

Source Tissue

Source Tissue Comment Organism Textmining
HL-60 cell vincristine-resistant leukemia cell line Homo sapiens
-
HL-60/Vinc cell vincristine-selected multidrug-resistant HL-60 cells Homo sapiens
-
additional information isozyme IIalpha is important in cell cycle kinetics in G2 and early M phases Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dsDNA + ATP + H2O decatenation of dsDNA Homo sapiens ?
-
 ?
additional information the amount of isozyme IIalpha in cells influences directly the cell cycle kinetics in G2 and early mitosis as well as the resistance to nocodazole Homo sapiens ?
-
 ?
additional information isozyme IIalpha interacts with the microtubule-directed agent nocodazole, overview Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
DNA topoisomerase II
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 decatenation assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 decatenation assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP required for decatenation activity Homo sapiens