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Literature summary for 5.6.2.2 extracted from
- A novel homogenous assay for topoisomerase II action and inhibition (2005), ChemBioChem, 6, 920-926.
Application
| Application | Comment | Organism |
|---|---|---|
| pharmacology | the enzyme is a target for anticancer drug development | Tequatrovirus T4 |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| m-AMSA | hydrochloride salt of amsacrine, topoisomerase poison | Tequatrovirus T4 |  |
| novobiocin | cumarin-based drug | Tequatrovirus T4 | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCl | - |
Tequatrovirus T4 | |
| Mg2+ | - |
Tequatrovirus T4 | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Tequatrovirus T4 | increased expression of the enzyme causes neoplasia | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Tequatrovirus T4 | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
development and evaluation of a dual-color fluorescence cross-correlation spectroscopy in homogeneous solution for monitoring enzyme activity and inhibitory effect of compounds, overview | Tequatrovirus T4 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dsDNA + ATP + H2O | cleavage and religation of dsDNA | Tequatrovirus T4 | ? | - |
? | |
| additional information | increased expression of the enzyme causes neoplasia | Tequatrovirus T4 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Topoisomerase II | - |
Tequatrovirus T4 |
| type II DNA topoisomerase | - |
Tequatrovirus T4 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at room temperature | Tequatrovirus T4 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
assay at | Tequatrovirus T4 |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Tequatrovirus T4 | |
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Release 2023.1 (January 2023)
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