Activating Compound | Comment | Organism | Structure |
---|---|---|---|
1,4-benzoquinone | a topoisomerase II poison in vivo and in vitro, enhances cleavage activity, inhibits ligation activity, at 0.025 mM, 1,4-hydroquinone has a higher inhibitory effect on ligation activity but a lower effect on cleavage activity compared to 1,4-benzoquinone | Homo sapiens | |
1,4-hydroquinone | physiological precursor of 1,4-benzoquinone, a topoisomerase II poison, enhances cleavage activity, inhibits ligation activity, at 0.25 mM, alters the enzyme's cleavage site specificity, 1,4-hydroquinone has a higher inhibitory effect on ligation activity but a lower effect on cleavage activity compared to 1,4-benzoquinone | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,4-benzoquinone | a topoisomerase II poison in vivo and in vitro, enhances cleavage activity, inhibits ligation activity, at 0.025 mM, 1,4-hydroquinone has a higher inhibitory effect on ligation activity but a lower effect on cleavage activity compared to 1,4-benzoquinone | Homo sapiens | |
1,4-hydroquinone | physiological precursor of 1,4-benzoquinone, a topoisomerase II poison, enhances cleavage activity, inhibits ligation activity, at 0.25 mM, alters the enzyme's cleavage site specificity, 1,4-hydroquinone has a higher inhibitory effect on ligation activity but a lower effect on cleavage activity compared to 1,4-benzoquinone | Homo sapiens | |
etoposide | - |
Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required for DNA ligation | Homo sapiens | |
KCl | required for DNA binding | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
double-stranded DNA + ATP + H2O | ATP-dependent breakage, passage, and rejoining of double-stranded DNA | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
topoisomerase II alpha | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.9 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |