Inhibitors | Comment | Organism | Structure |
---|---|---|---|
azalactone ferrocene | specific inhibition of DNA passage and ATPase activities of isozyme IIbeta with IC50 of 0.1 mM, inhibition mechanism via complex formation, overview | Rattus norvegicus | |
additional information | inhibitory potency of diverse ferrocene derivatives, overview | Rattus norvegicus | |
thiomorpholide amido methyl ferrocene | specific inhibition of DNA passage and ATPase activities of isozyme IIbeta with IC50 of 0.05 mM, inhibition mechanism via complex formation, overview | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | - |
Rattus norvegicus | |
Mg2+ | - |
Rattus norvegicus | |
NaCl | required for DNA cleavage | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
isozymes IIalpha and IIbeta from cerebellum by 2 steps of ion exchange chromatography, and immunoaffinity chromatography | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
cerebellum | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme shows ATPase activity | Rattus norvegicus | ? | - |
? | |
pBR322 DNA + ATP + H2O | cleavage of pBR322 DNA | Rattus norvegicus | ? | - |
? | |
supercoiled pRYG DNA + ATP + H2O | relaxation of supercoiled pRYG DNA | Rattus norvegicus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
topoisomerase IIalpha | - |
Rattus norvegicus |
topoisomerase IIbeta | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
ATPase assay at | Rattus norvegicus |
7.9 | - |
DNA cleavage assay at | Rattus norvegicus |
8 | - |
DNA relaxation assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | required for DNA relaxation and ATPase activities | Rattus norvegicus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.05 | - |
specific inhibition of DNA passage and ATPase activities of isozyme IIbeta with IC50 of 0.05 mM, inhibition mechanism via complex formation, overview | Rattus norvegicus | thiomorpholide amido methyl ferrocene | |
0.1 | - |
specific inhibition of DNA passage and ATPase activities of isozyme IIbeta with IC50 of 0.1 mM, inhibition mechanism via complex formation, overview | Rattus norvegicus | azalactone ferrocene |