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Literature summary for 5.6.2.2 extracted from
- Purification and functional characterization of type II DNA topoisomerase from rat testis and comparison with topoisomerase II from liver (1996), Biochim. Biophys. Acta, 1308, 58-66.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| potassium glutamate | 1 M, inhibition of relaxation activity | Rattus norvegicus |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Rattus norvegicus | |
| Mg2+ | optimal decatenation activity at 5-10 mM | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 150000 | - |
1 * 150000, testis enzyme, SDS-PAGE | Rattus norvegicus |
| 160000 | - |
1 * 160000, liver enzyme, SDS-PAGE | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Rattus norvegicus | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
| testis | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, 40% glycerol, stable for about 1 year | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | - |
Rattus norvegicus | ? | - |
? | |
| network of DNA rings + ATP + H2O | decatenation | Rattus norvegicus | monomeric DNA circles + ADP + phosphate | - |
? | |
| supercoiled DNA + ATP + H2O | catenation | Rattus norvegicus | catenated DNA networks + ADP + phosphate | - |
? | |
| supercoiled DNA + ATP + H2O | relaxation | Rattus norvegicus | relaxed DNA + ADP + phosphate | - |
? | |
| supercoiled DNA + ATP + H2O | ATP-dependent relaxation | Rattus norvegicus | relaxed DNA + ADP + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 150000, testis enzyme, SDS-PAGE | Rattus norvegicus |
| monomer | 1 * 160000, liver enzyme, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 28 | 40 | decatenation activity | Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7.5 | liver enzyme, decatenation activity | Rattus norvegicus |
| 6.5 | 8.5 | testis enzyme, decatenation activity | Rattus norvegicus |
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