KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km for double-stranded DNA is 0.0015 mg/ml, 0.0045 mM nucleotides | Tequatrovirus T4 |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | the products of gene 39, MW 57000, and gene 52, MW 48000, are tightly membrane-associated | Tequatrovirus T4 | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | required | Tequatrovirus T4 | |
Mg2+ | required | Tequatrovirus T4 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18000 | - |
2 * 18000, gene 60 protein, + 2 * 48000, gene 52 protein, + 2 * 57000, gene 39 protein, SDS-PAGE | Tequatrovirus T4 |
48000 | - |
2 * 18000, gene 60 protein, + 2 * 48000, gene 52 protein, + 2 * 57000, gene 39 protein, SDS-PAGE | Tequatrovirus T4 |
57000 | - |
2 * 18000, gene 60 protein, + 2 * 48000, gene 52 protein, + 2 * 57000, gene 39 protein, SDS-PAGE | Tequatrovirus T4 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tequatrovirus T4 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Tequatrovirus T4 |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | E. coli, bacteriophage T4-infected | Tequatrovirus T4 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Tequatrovirus T4 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme also cleaves single-stranded DNA in a site-specific fashion, but this cleavage is mechanistically distinct from the double-strand DNA cleavage | Tequatrovirus T4 | ? | - |
? | |
additional information | cannot supercoil various E. coli plasmid DNAs in vitro | Tequatrovirus T4 | ? | - |
? | |
network of DNA rings + ATP + H2O | decatenation | Tequatrovirus T4 | monomeric DNA circles + ADP + phosphate | - |
? | |
supercoiled DNA + ATP + H2O | catenation | Tequatrovirus T4 | catenated DNA networks + ADP + phosphate | - |
? | |
supercoiled DNA + ATP + H2O | relaxation | Tequatrovirus T4 | relaxed DNA + ADP + phosphate | ADP is cleaved to ADP and phosphate | ? | |
supercoiled DNA + ATP + H2O | relaxation of negatively or positively supercoiled DNA | Tequatrovirus T4 | relaxed DNA + ADP + phosphate | ADP is cleaved to ADP and phosphate | ? | |
supercoiled DNA + ATP + H2O | pBR322 plasmid DNA | Tequatrovirus T4 | relaxed DNA + ADP + phosphate | ADP is cleaved to ADP and phosphate | ? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 2 * 18000, gene 60 protein, + 2 * 48000, gene 52 protein, + 2 * 57000, gene 39 protein, SDS-PAGE | Tequatrovirus T4 |