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Literature summary for 5.6.1.8 extracted from

  • Matusovsky, O.S.; Shevchenko, U.V.; Matusovskaya, G.G.; Sobieszek, A.; Dobrzhanskaya, A.V.; Sheludko, N.S.
    Catch muscle myorod modulates ATPase activity of myosin in a phosphorylation-dependent way (2015), PLoS ONE, 10, e0125379.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.6.1.8

Activating Compound

Activating Compound Comment Organism Structure
myorod myorod is expressed exclusively in molluscan catch muscle and localizes on the surface of thick filaments together with twitchin and myosin. Myorod is an alternatively spliced product of themyosin heavy-chain gene that contains the C-terminal rod part of myosin and a unique N-terminal domain. Phosphorylation at the N-terminus of myorod potentiates the actin-activated Mg2+-ATPase activity of mussel myosin, no activation by unphosphorylated myorod. Actomyosin reconstitution from rabbit F-actin and mussel myosin in presence or absence of unphosphorylated or phosphorylated myorod is prepared in two modes, overview Crenomytilus grayanus
myorod myorod is expressed exclusively in molluscan catch muscle and localizes on the surface of thick filaments together with twitchin and myosin. Myorod is an alternatively spliced product of themyosin heavy-chain gene that contains the C-terminal rod part of myosin and a unique N-terminal domain. Phosphorylation at the N-terminus of myorod potentiates the actin-activated Mg2+-ATPase activity of rabbit myosin, no activation by unphosphorylated myorod. Actomyosin reconstitution from rabbit F-actin and rabbit myosin in presence or absence of unphosphorylated or phosphorylated myorod is prepared in two modes, overview Oryctolagus cuniculus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Oryctolagus cuniculus
Mg2+ required Crenomytilus grayanus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + myosin bound to actin filament at position n Oryctolagus cuniculus
-
 ADP + phosphate + myosin bound to actin filament at position n+1
-
 ?
ATP + H2O + myosin bound to actin filament at position n Crenomytilus grayanus
-
 ADP + phosphate + myosin bound to actin filament at position n+1
-
 ?

Organism

Organism UniProt Comment Textmining
Crenomytilus grayanus
-
-
-
Oryctolagus cuniculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native myosin and myorod from posterior adductor by ammonium sulfate fractionation, dialysis, and gel filtration Crenomytilus grayanus

Source Tissue

Source Tissue Comment Organism Textmining
posterior adductor muscle
-
 Crenomytilus grayanus
-
skeletal muscle
-
 Oryctolagus cuniculus
-
smooth muscle
-
 Crenomytilus grayanus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + myosin bound to actin filament at position n
-
 Oryctolagus cuniculus ADP + phosphate + myosin bound to actin filament at position n+1
-
 ?
ATP + H2O + myosin bound to actin filament at position n
-
 Crenomytilus grayanus ADP + phosphate + myosin bound to actin filament at position n+1
-
 ?

Synonyms

Synonyms Comment Organism
actomyosin
-
 Oryctolagus cuniculus
actomyosin
-
 Crenomytilus grayanus
myosin
-
 Oryctolagus cuniculus
myosin
-
 Crenomytilus grayanus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Oryctolagus cuniculus
25
-
 assay at Crenomytilus grayanus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Oryctolagus cuniculus
7.2
-
 assay at Crenomytilus grayanus

General Information

General Information Comment Organism
additional information actomyosin reconstitution from rabbit F-actin and mussel myosin in presence or absence of unphosphorylated or phosphorylated myorod is prepared in two modes, overview Crenomytilus grayanus
additional information actomyosin reconstitution from rabbit F-actin and rabbit myosin in presence or absence of unphosphorylated or phosphorylated myorod is prepared in two modes, overview Oryctolagus cuniculus