Literature summary for 5.6.1.6 extracted from

Beck, E.J.; Yang, Y.; Yaemsiri, S.; Raghuram, V.
Conformational changes in a pore-lining helix coupled to cystic fibrosis transmembrane conductance regulator channel gating (2008), J. Biol. Chem., 283, 4957-4966.

Search for more literature for 5.6.1.6

Protein Variants

Protein Variants	Comment	Organism
E1371Q	mutation in nucleotide-binding domain 2, prevents ATP hydrolysis without affecting ATP binding and stabilizes the open state of the channel by almost 1000fold	Homo sapiens
I331C	modification rate by methanethiosulfonate ethyl ammonium and (2-sulfonatoethyl) methanethiosulfonate is slower in the open state than in the closed state	Homo sapiens
K335C	modification rate by methanethiosulfonate ethyl ammonium and (2-sulfonatoethyl) methanethiosulfonate is slower in the open state than in the closed state	Homo sapiens
L333C	modification rate by methanethiosulfonate ethyl ammonium and (2-sulfonatoethyl) methanethiosulfonate is slower in the open state than in the closed state	Homo sapiens
additional information	construction of 26 Cys-substituted mutant and analysis of change in cAMP-activated whole cell conductance	Homo sapiens
R334C	modification rate by methanethiosulfonate ethyl ammonium and (2-sulfonatoethyl) methanethiosulfonate is not dependent on open/closed state	Homo sapiens
T338C	modification rate by methanethiosulfonate ethyl ammonium and (2-sulfonatoethyl) methanethiosulfonate is slower in the open state than in the closed state	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	isoform CFTR	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + closed Cl- channel	ATP binding induces a modest conformational change in the sixth transmembrane segment, and this conformational change is coupled to the gating mechanism that regulates ion conduction	Homo sapiens	ADP + phosphate + open Cl- channel	-	?

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Release 2023.1 (January 2023)