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Literature summary for 5.6.1.2 extracted from
- Biochemical identification of dynein-ATPase activity in human sperm (2009), Z. Naturforsch. C, 64, 747-753.
Application
| Application | Comment | Organism |
|---|---|---|
| diagnostics | the straightforward dynein-ATPase assay can be used to obtain data of functional interest in clinical or experimental settings, to test the effect of new drugs that could inhibit dynein-ATPase actitivty and therefor sperm motility | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | in presence of 1 mM, 12% activity compared to assay without EDTA | Homo sapiens |  |
| Li+ | 6 mM, 72% inhibition | Homo sapiens | |
| additional information | insensitive to 2 mM ouabain, 0.001 mM oligomycin, and 0.001 mM thapsgargin | Homo sapiens | |
| vanadate | inhibited by 0.01 mM, this inhibition is reversed in the presence of 1 mM norepinephrine, by reducing vanadate to oxovanadium | Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | dependent on | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| sperm | axenome-containg fractions, free of plasma membranes, dynein ATPase is the intracelluar motor for sperm motility, from healthy donors with either normozoospermia or asthenozoospermia, as indicated by a sperm motility lower than 50% (WHO grade a + b) | Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the dynein-ATPase activity is significantly lower for asthenozoospermic donors as compared to normozoospermic dononors | Homo sapiens |
| 0.026 | - |
containing 2 mM MgCl2, pH 8.0. 37°C | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| dynein-ATPase | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Homo sapiens |
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Release 2023.1 (January 2023)
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