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Literature summary for 5.6.1.2 extracted from

  • Shima, T.; Kon, T.; Imamula, K.; Ohkura, R.; Sutoh, K.
    Two modes of microtubule sliding driven by cytoplasmic dynein (2006), Proc. Natl. Acad. Sci. USA, 103, 17736-17740.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.6.1.2

Cloned(Commentary)

Cloned (Comment) Organism
expression of full-length or 380 kDa fragment of dynein heavy chain with His-FLAG-green fluorescent protein tandem tags or with His-FLAG-biotin tandem tags Dictyostelium discoideum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
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 Dictyostelium discoideum 5737
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Organism

Organism UniProt Comment Textmining
Dictyostelium discoideum
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-
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
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 in vitro motility assay using biotinylated cytoplasmic dyneins anchored on a glass surface Dictyostelium discoideum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information robust microtubule sliding of dynein is driven by the power stroke of dynein tail. Slow microtubule sliding is independent of dynein orientation on a glass surface and is mechanically distinct from the sliding driven by the powerstroke of the tail Dictyostelium discoideum ?
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