Literature summary for 5.5.1.B7 extracted from

Pelot, K.A.; Mitchell, R.; Kwon, M.; Hagelthorn, D.M.; Wardman, J.F.; Chiang, A.; Bohlmann, J.; Ro, D.K.; Zerbe, P.
Biosynthesis of the psychotropic plant diterpene salvinorin A: Discovery and characterization of the Salvia divinorum clerodienyl diphosphate synthase (2017), Plant J., 89, 885-897.

Search for more literature for 5.5.1.B7

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Nicotiana benthamiana and Escherichia coli BL21DE3-C41 cells	Salvia divinorum

Protein Variants

Protein Variants	Comment	Organism
F255A/N313A	the mutation directs product specificity completely towards formation of ent-8alpha/betalambda-13E-en-8,15-diol	Salvia divinorum
F255H	the mutation alters the product outcome from (-)-kolavenol to ent-copalol as the sole product	Salvia divinorum

Organism

Organism	UniProt	Comment	Textmining
Salvia divinorum	A0A1L3THX5	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni2+-affinity column chromatography and Superdex 200 gel filtration	Salvia divinorum

Source Tissue

Source Tissue	Comment	Organism	Textmining
trichome	specific expression in trichomes	Salvia divinorum	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
geranylgeranyl diphosphate	-	Salvia divinorum	(-)-kolavenol	-	?
geranylgeranyl diphosphate	the reaction proceeds through conversion of the precursor geranylgeranyl diphosphate into the clerodienyl diphosphate intermediate via a series of 1,2-hydride and methyl shifts of the intermediary labda-13-en-8-yl diphosphate intermediate	Salvia divinorum	clerodienyl diphosphate + H+	-	?

Synonyms

Synonyms	Comment	Organism
clerodienyl diphosphate synthase	-	Salvia divinorum
CLPP synthase	-	Salvia divinorum
CPS2	-	Salvia divinorum

General Information

General Information	Comment	Organism
metabolism	the enzyme catalyzes the committed step in salvinorin A biosynthesis	Salvia divinorum

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Release 2023.1 (January 2023)