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Literature summary for 5.5.1.B7 extracted from

  • Pelot, K.A.; Mitchell, R.; Kwon, M.; Hagelthorn, D.M.; Wardman, J.F.; Chiang, A.; Bohlmann, J.; Ro, D.K.; Zerbe, P.
    Biosynthesis of the psychotropic plant diterpene salvinorin A: Discovery and characterization of the Salvia divinorum clerodienyl diphosphate synthase (2017), Plant J., 89, 885-897.
    View publication on PubMed
Search for more literature for 5.5.1.B7

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Nicotiana benthamiana and Escherichia coli BL21DE3-C41 cells Salvia divinorum

Protein Variants

Protein Variants Comment Organism
F255A/N313A the mutation directs product specificity completely towards formation of ent-8alpha/betalambda-13E-en-8,15-diol Salvia divinorum
F255H the mutation alters the product outcome from (-)-kolavenol to ent-copalol as the sole product Salvia divinorum

Organism

Organism UniProt Comment Textmining
Salvia divinorum A0A1L3THX5
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-affinity column chromatography and Superdex 200 gel filtration Salvia divinorum

Source Tissue

Source Tissue Comment Organism Textmining
trichome specific expression in trichomes Salvia divinorum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
geranylgeranyl diphosphate
-
 Salvia divinorum (-)-kolavenol
-
 ?
geranylgeranyl diphosphate the reaction proceeds through conversion of the precursor geranylgeranyl diphosphate into the clerodienyl diphosphate intermediate via a series of 1,2-hydride and methyl shifts of the intermediary labda-13-en-8-yl diphosphate intermediate Salvia divinorum clerodienyl diphosphate + H+
-
 ?

Synonyms

Synonyms Comment Organism
clerodienyl diphosphate synthase
-
 Salvia divinorum
CLPP synthase
-
 Salvia divinorum
CPS2
-
 Salvia divinorum

General Information

General Information Comment Organism
metabolism the enzyme catalyzes the committed step in salvinorin A biosynthesis Salvia divinorum