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EC unknown
Synonyms
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clerodienyl diphosphate synthase
A0A1L3THX5
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CLPP synthase
A0A1L3THX5
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ent-neo-CT-CLPP synthase
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geranylgeranyl diphosphate = (-)-kolavenol
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geranylgeranyl diphosphate-lyase (cyclizing, (-)-kolavenol-forming)
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(E,E,E)-geranylgeranyl diphosphate
cis,trans-clerodienyl diphosphate
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Substrates: -
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(Z,Z,Z)-nerylneryl diphosphate
(5S,8S,9R,10R)-13Z-clerodienyl diphosphate
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Substrates: -
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geranylgeranyl diphosphate
(-)-kolavenol
A0A1L3THX5
Substrates: -
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geranylgeranyl diphosphate
clerodienyl diphosphate + H+
A0A1L3THX5
Substrates: the reaction proceeds through conversion of the precursor geranylgeranyl diphosphate into the clerodienyl diphosphate intermediate via a series of 1,2-hydride and methyl shifts of the intermediary labda-13-en-8-yl diphosphate intermediate
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geranylgeranyl diphosphate
ent-neo-cis-trans-clerodienyl diphosphate
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Substrates: -
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(E,E,E)-geranylgeranyl diphosphate
cis,trans-clerodienyl diphosphate
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Substrates: -
?
geranylgeranyl diphosphate
ent-neo-cis-trans-clerodienyl diphosphate
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Substrates: -
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brenda
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A0A1L3THX5
UniProt
brenda
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A0A1L3THX5
specific expression in trichomes
brenda
Highest Expressing Human Cell Lines
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Cell Line Links
Gene Links
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metabolism
A0A1L3THX5
the enzyme catalyzes the committed step in salvinorin A biosynthesis
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F251A
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the mutant produces (5S,8S,9R,10R)-13Z-clerodienyl diphosphate from (Z,Z,Z)-nerylneryl diphosphate with a 3fold increased activity compared to the wild type enzyme. The enzyme also produces cis,cis-clerodienyl diphosphate besides the natural product cis,trans-clerodienyl diphosphate from (E,E,E)-geranylgeranyl diphosphate
F251G
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the mutant shows reduced activity towards (E,E,E)-geranylgeranyl diphosphate compared to the wild type enzyme
F251S
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the mutant shows reduced activity towards (E,E,E)-geranylgeranyl diphosphate compared to the wild type enzyme
F251V
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the mutant produces (5S,8S,9R,10R)-13Z-clerodienyl diphosphate from (Z,Z,Z)-nerylneryl diphosphate with an 8fold increased activity compared to the wild type enzyme. The enzyme also produces cis,cis-clerodienyl diphosphate besides the natural product cis,trans-clerodienyl diphosphate from (E,E,E)-geranylgeranyl diphosphate
F357G
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the mutant produces syn-halima-(5,13E)-dienyl diphosphate from geranylgeranyl diphosphate
F255A/N313A
A0A1L3THX5
the mutation directs product specificity completely towards formation of ent-8alpha/betalambda-13E-en-8,15-diol
F255H
A0A1L3THX5
the mutation alters the product outcome from (-)-kolavenol to ent-copalol as the sole product
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Ni2+-affinity column chromatography and Superdex 200 gel filtration
A0A1L3THX5
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expressed in Escherichia coli
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expressed in Nicotiana benthamiana and Escherichia coli BL21DE3-C41 cells
A0A1L3THX5
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Pelot, K.A.; Mitchell, R.; Kwon, M.; Hagelthorn, D.M.; Wardman, J.F.; Chiang, A.; Bohlmann, J.; Ro, D.K.; Zerbe, P.
Biosynthesis of the psychotropic plant diterpene salvinorin A: Discovery and characterization of the Salvia divinorum clerodienyl diphosphate synthase
Plant J.
89
885-897
2017
Salvia divinorum (A0A1L3THX5), Salvia divinorum
brenda
Pelot, K.A.; Hagelthorn, D.M.; Hong, Y.J.; Tantillo, D.J.; Zerbe, P.
Diterpene synthase-catalyzed biosynthesis of distinct clerodane stereoisomers
Chembiochem
20
111-117
2020
Panicum virgatum
brenda
Pelot, K.; Chen, R.; Hagelthorn, D.; Young, C.; Addison, J.; Muchlinski, A.; Tholl, D.; Zerbe, P.
Functional diversity of diterpene synthases in the biofuel crop switchgrass
Plant Physiol.
178
54-71
2018
Panicum virgatum
brenda
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