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Literature summary for 5.5.1.23 extracted from
- DnrD cyclase involved in the biosynthesis of doxorubicin: purification and characterization of the recombinant enzyme (1999), Biochemistry, 38, 4794-4799.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpressed in Escherichia coli | Streptomyces peucetius |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | enzyme activity is not accelerated or significantly inhibited by divalent metal ions (Zn2+, Co2+, Fe2+, Cu2+, or Mn2+) or chelating reagents | Streptomyces peucetius |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0521 | - |
methyl aklanonate | pH 7.0, 30°C | Streptomyces peucetius |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | enzyme activity is not accelerated or significantly inhibited by divalent metal ions (Zn2+, Co2+, Fe2+, Cu2+, or Mn2+) or chelating reagents | Streptomyces peucetius |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 16000 | - |
x * 16000, SDS-PAGE | Streptomyces peucetius |
| 16572 | - |
x * 16572, electrospray MS analysis | Streptomyces peucetius |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| methyl aklanonate | Streptomyces peucetius | - |
aklaviketone | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces peucetius | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Streptomyces peucetius |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 14.3 | - |
pH 7.0, 30°C | Streptomyces peucetius |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| methyl aklanonate | - |
Streptomyces peucetius | aklaviketone | - |
? | |
| methyl aklanonate | base-catalyzed mechanism | Streptomyces peucetius | aklaviketone | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 16000, SDS-PAGE | Streptomyces peucetius |
| ? | x * 16572, electrospray MS analysis | Streptomyces peucetius |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AAMEcyclase | - |
Streptomyces peucetius |
| DnrD | - |
Streptomyces peucetius |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Streptomyces peucetius |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Streptomyces peucetius |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9 | pH 6.5: about 65% of maximal activity, 9.0: about 30% of maximal activity | Streptomyces peucetius |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme is involved in biosynthesis of aklaviketone, an intermediate in the biosynthetic pathway to daunorubicin and doxorubicin | Streptomyces peucetius |
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Release 2023.1 (January 2023)
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