Protein Variants | Comment | Organism |
---|---|---|
A271S | ratio of turnover number to Km-value: 1.5fold decrease with cis,cis-muconate as substrate, 3.3fold decrease with 2-chloro-cis,cis-muconate as substrate, 27fold increase with 3-chloro-cis,cis-muconate as substrate, 1.9fold decrease with 2,4-dichloro-cis,cis-muconate as substrate, 1.5fold decrease with 3-fluoro-cis,cis-muconate as substrate, 1.1fold increase with 2-methyl-cis,cis-muconate as substrate, 1.1fold decrease with 3-methyl-cis,cis-muconate as substrate | Pseudomonas putida |
F329I | ratio of turnover number to Km-value: 5.7fold decrease with cis,cis-muconate as substrate, 1.6fold increase with 2-chloro-cis,cis-muconate as substrate, 3.4fold increase with 3-chloro-cis,cis-muconate as substrate, 3.2fold increase with 2,4-dichloro-cis,cis-muconate as substrate, 1.7fold decrease with 3-fluoro-cis,cis-muconate as substrate, 1.5fold increase with 2-methyl-cis,cis-muconate as substrate, 5.8fold decrease with 3-methyl-cis,cis-muconate as substrate | Pseudomonas putida |
I54V | ratio of turnover number to Km-value: 11.9fold decrease with cis,cis-muconate as substrate, nearly identical to wild-type value with 2-chloro-cis,cis-muconate and 3-methyl-cis,cis-muconate as substrate, 22fold increase with 3-chloro-cis,cis-muconate as substrate, 1.67fold decrease with 2,4-dichloro-cis,cis-muconate as substrate, 5.8fold decrease with 3-fluoro-cis,cis-muconate as substrate, 8.8fold decrease with 2-methyl-cis,cis-muconate as substrate | Pseudomonas putida |
I54V/F329I | ratio of turnover number to Km-value: 81.5fold decrease with cis,cis-muconate as substrate, 2.6fold decrease with 2-chloro-cis,cis-muconate as substrate, 2.7fold increase with 3-chloro-cis,cis-muconate as substrate, 3.8fold increase with 2,4-dichloro-cis,cis-muconate as substrate, 2fold decrease with 3-fluoro-cis,cis-muconate as substrate, 4fold decrease with 2-methyl-cis,cis-muconate as substrate, 2fold decrease with 3-methyl-cis,cis-muconate as substrate | Pseudomonas putida |
K276N | ratio of turnover number to Km-value: 10.7fold decrease with cis,cis-muconate as substrate, 8.7fold decrease with 2-chloro-cis,cis-muconate as substrate, 3.5fold increase with 3-chloro-cis,cis-muconate as substrate, 8.7fold decrease with 2,4-dichloro-cis,cis-muconate as substrate, 4.1fold decrease with 3-fluoro-cis,cis-muconate as substrate, 16.7fold decrease with 2-methyl-cis,cis-muconate as substrate, 12.1fold decrease with 3-methyl-cis,cis-muconate as substrate | Pseudomonas putida |
L333V | ratio of turnover number to Km-value: 238fold decrease with cis,cis-muconate as substrate, 41.5fold decrease with 2-chloro-cis,cis-muconate as substrate, 3.4fold increase with 3-chloro-cis,cis-muconate as substrate, 5.9fold decrease with 2,4-dichloro-cis,cis-muconate as substrate, 1.4fold decrease with 3-fluoro-cis,cis-muconate as substrate, 34.6fold decrease with 2-methyl-cis,cis-muconate as substrate, 16.5fold decrease with 3-methyl-cis,cis-muconate as substrate | Pseudomonas putida |
Y59W | ratio of turnover number to Km-value: 12.9fold decrease with cis,cis-muconate as substrate, nearly identical to wild-type value with 2-chloro-cis,cis-muconate as substrate, 4.4fold decrease with 3-fluoro-cis,cis-muconate, 1.8fold decrease with 2-methyl-cis,cis-muconate as substrate, 10.2fold decrease with 3-methyl-cis,cis-muconate as substrate | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,4-dichloro-cis,cis-muconate | inhibitor of cis,cis-muconate conversion | Acinetobacter calcoaceticus | |
2,4-dichloro-cis,cis-muconate | inhibitor of cis,cis-muconate conversion | Pseudomonas putida | |
2-fluoro-cis,cis-muconate | inhibitor of cis,cis-muconate conversion | Acinetobacter calcoaceticus | |
2-fluoro-cis,cis-muconate | inhibitor of cis,cis-muconate conversion | Pseudomonas putida | |
3-chloro-cis,cis-muconate | inhibitor of cis,cis-muconate conversion | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
3-methyl-cis,cis-muconate | pH 7.5, 25°C, wild-type enzyme | Pseudomonas putida | |
0.0064 | - |
3-methyl-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme A271S or I54V/F329 | Pseudomonas putida | |
0.0072 | - |
3-methyl-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme I54V | Pseudomonas putida | |
0.0091 | - |
3-methyl-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme L333V | Pseudomonas putida | |
0.0097 | - |
3-methyl-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme A271S | Pseudomonas putida | |
0.012 | - |
3-methyl-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme K276N | Pseudomonas putida | |
0.015 | - |
3-methyl-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme Y59W | Pseudomonas putida | |
0.016 | - |
3-methyl-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme F329I | Pseudomonas putida | |
0.017 | - |
2-chloro-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme Y59W | Pseudomonas putida | |
0.026 | - |
2,4-dichloro-cis,cis-muconate | pH 7.5, 25°C, wild-type enzyme | Pseudomonas putida | |
0.033 | - |
2-chloro-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme I54V/F329I | Pseudomonas putida | |
0.034 | - |
2-chloro-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme K276N | Pseudomonas putida | |
0.035 | - |
cis,cis-muconate | pH 7.5, 25°C, mutant enzyme A271S | Pseudomonas putida | |
0.036 | - |
2-chloro-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme L333V | Pseudomonas putida | |
0.037 | - |
2-chloro-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme F329I | Pseudomonas putida | |
0.039 | - |
2,4-dichloro-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme L333V | Pseudomonas putida | |
0.04 | - |
2,4-dichloro-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme K276N | Pseudomonas putida | |
0.04 | - |
cis,cis-muconate | pH 7.5, 25°C, wild-type enzyme | Pseudomonas putida | |
0.04 | - |
2-methyl-cis,cis-muconate | pH 7.5, 25°C, wild-type enzyme | Pseudomonas putida | |
0.044 | - |
2,4-dichloro-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme A271S | Pseudomonas putida | |
0.045 | - |
3-chloro-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme I54V | Pseudomonas putida | |
0.045 | - |
2,4-dichloro-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme I54V/F329I | Pseudomonas putida | |
0.047 | - |
2-chloro-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme I54V | Pseudomonas putida | |
0.047 | - |
cis,cis-muconate | pH 7.5, 25°C, mutant enzyme Y59W and F329I | Pseudomonas putida | |
0.052 | - |
cis,cis-muconate | pH 7.5, 25°C, mutant enzyme L333V | Pseudomonas putida | |
0.057 | - |
2-methyl-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme A271S or I54V/F329 | Pseudomonas putida | |
0.058 | - |
2-methyl-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme I54V | Pseudomonas putida | |
0.059 | - |
2-methyl-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme A271S | Pseudomonas putida | |
0.059 | - |
2-chloro-cis,cis-muconate | pH 7.5, 25°C, wild-type enzyme | Pseudomonas putida | |
0.06 | - |
2-chloro-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme A271S | Pseudomonas putida | |
0.063 | - |
3-fluoro-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme L333V | Pseudomonas putida | |
0.068 | - |
2-methyl-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme F329I | Pseudomonas putida | |
0.069 | - |
2-methyl-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme Y59W | Pseudomonas putida | |
0.074 | - |
2,4-dichloro-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme I54V | Pseudomonas putida | |
0.074 | - |
cis,cis-muconate | pH 7.5, 25°C, mutant enzyme K276N | Pseudomonas putida | |
0.076 | - |
3-chloro-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme L333V | Pseudomonas putida | |
0.078 | - |
2,4-dichloro-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme F329I | Pseudomonas putida | |
0.093 | - |
2,4-dichloro-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme I54V | Pseudomonas putida | |
0.096 | - |
cis,cis-muconate | pH 7.5, 25°C, mutant enzyme I54V | Pseudomonas putida | |
0.097 | - |
3-fluoro-cis,cis-muconate | pH 7.5, 25°C, wild-type enzyme | Pseudomonas putida | |
0.099 | - |
3-methyl-cis,cis-muconate | pH 7.5, 25°C | Acinetobacter calcoaceticus | |
0.1 | - |
3-fluoro-cis,cis-muconate | pH 7.5, 25°C | Acinetobacter calcoaceticus | |
0.1 | - |
3-fluoro-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme A271S or I54V/F329 | Pseudomonas putida | |
0.11 | - |
2-methyl-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme L333V | Pseudomonas putida | |
0.13 | - |
cis,cis-muconate | pH 7.5, 25°C | Acinetobacter calcoaceticus | |
0.14 | - |
cis,cis-muconate | pH 7.5, 25°C, mutant enzyme I54V/F329I | Pseudomonas putida | |
0.15 | - |
3-fluoro-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme F329I | Pseudomonas putida | |
0.15 | - |
3-fluoro-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme Y59W | Pseudomonas putida | |
0.18 | - |
3-chloro-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme A271S | Pseudomonas putida | |
0.18 | - |
2-methyl-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme K276N | Pseudomonas putida | |
0.21 | - |
3-chloro-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme I54V/F329I | Pseudomonas putida | |
0.23 | - |
3-fluoro-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme K276N | Pseudomonas putida | |
0.29 | - |
2-chloro-cis,cis-muconate | pH 7.5, 25°C | Acinetobacter calcoaceticus | |
0.32 | - |
3-chloro-cis,cis-muconate | pH 7.5, 25°C, wild-type enzyme | Pseudomonas putida | |
0.49 | - |
2-methyl-cis,cis-muconate | pH 7.5, 25°C | Acinetobacter calcoaceticus | |
0.55 | - |
3-chloro-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme K276N | Pseudomonas putida | |
0.69 | - |
3-chloro-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme F329I | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cis,cis-muconate | Pseudomonas putida | the enzyme plays a crucial role in the bacterial degradation of aromatic compounds by converting cis,cis-muconate, the product of catechol ring cleavage to (4S)-muconolactone | muconolactone | - |
? | |
cis,cis-muconate | Acinetobacter calcoaceticus | the enzyme plays a crucial role in the bacterial degradation of aromatic compounds by converting cis,cis-muconate, the product of catechol ring cleavage to (4S)-muconolactone | muconolactone | - |
? | |
cis,cis-muconate | Pseudomonas putida PRS2000 | the enzyme plays a crucial role in the bacterial degradation of aromatic compounds by converting cis,cis-muconate, the product of catechol ring cleavage to (4S)-muconolactone | muconolactone | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter calcoaceticus | - |
- |
- |
Pseudomonas putida | - |
PRS2000 | - |
Pseudomonas putida PRS2000 | - |
PRS2000 | - |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
- |
Acinetobacter calcoaceticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4-dichloro-cis,cis-muconate | - |
Pseudomonas putida | ? | - |
? | |
2,4-dichloro-cis,cis-muconate | - |
Pseudomonas putida PRS2000 | ? | - |
? | |
2-Chloro-cis,cis-muconate | - |
Acinetobacter calcoaceticus | ? | - |
? | |
2-chloro-cis,cis-muconate | - |
Pseudomonas putida | (+)-2-chloromuconolactone + (+)-5-chloromuconolactone | mutant enzymes I54V and I54V/Y59W form 5-chloromuconolactone almost exclusively. Mutant enzymes F329I and Y59W form 2-chloromuconolactone as the main product. Mutant enzyme A271S hehaves similar to wild-type enzyme | ? | |
2-Methyl-cis,cis-muconate | - |
Acinetobacter calcoaceticus | ? | - |
? | |
2-methyl-cis,cis-muconate | - |
Pseudomonas putida | 2-methylmuconolactone | - |
? | |
3-chloro-cis,cis-muconate | - |
Pseudomonas putida | protoanemonin + ? | formed by wild-type enzyme and mutant enzymes I54V, A271S, K276N, F329I, L333V, I54V/F329I | ? | |
3-Fluoro-cis,cis-muconate | - |
Pseudomonas putida | ? | - |
? | |
3-Fluoro-cis,cis-muconate | - |
Acinetobacter calcoaceticus | ? | - |
? | |
3-Fluoro-cis,cis-muconate | - |
Pseudomonas putida PRS2000 | ? | - |
? | |
3-Methyl-cis,cis-muconate | - |
Pseudomonas putida | ? | - |
? | |
3-Methyl-cis,cis-muconate | - |
Acinetobacter calcoaceticus | ? | - |
? | |
3-Methyl-cis,cis-muconate | - |
Pseudomonas putida PRS2000 | ? | - |
? | |
cis,cis-muconate | - |
Pseudomonas putida | muconolactone | - |
? | |
cis,cis-muconate | - |
Acinetobacter calcoaceticus | muconolactone | - |
? | |
cis,cis-muconate | the enzyme plays a crucial role in the bacterial degradation of aromatic compounds by converting cis,cis-muconate, the product of catechol ring cleavage to (4S)-muconolactone | Pseudomonas putida | muconolactone | - |
? | |
cis,cis-muconate | the enzyme plays a crucial role in the bacterial degradation of aromatic compounds by converting cis,cis-muconate, the product of catechol ring cleavage to (4S)-muconolactone | Acinetobacter calcoaceticus | muconolactone | - |
? | |
cis,cis-muconate | - |
Pseudomonas putida PRS2000 | muconolactone | - |
? | |
cis,cis-muconate | the enzyme plays a crucial role in the bacterial degradation of aromatic compounds by converting cis,cis-muconate, the product of catechol ring cleavage to (4S)-muconolactone | Pseudomonas putida PRS2000 | muconolactone | - |
? | |
additional information | no activity with 3-chloro-cis,cis-muconate and 2,4-dichloro-cis,cis-muconate | Acinetobacter calcoaceticus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ADP1 | - |
Acinetobacter calcoaceticus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
2,4-dichloro-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme L333V | Pseudomonas putida | |
3.4 | - |
2,4-dichloro-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme K276N | Pseudomonas putida | |
36.6 | - |
2,4-dichloro-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme K276N | Pseudomonas putida | |
43.8 | - |
2-chloro-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme L333V | Pseudomonas putida | |
52.8 | - |
2,4-dichloro-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme L333V | Pseudomonas putida | |
174 | - |
2,4-dichloro-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme A271S | Pseudomonas putida | |
192 | - |
2-chloro-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme K276N | Pseudomonas putida | |
198 | - |
2,4-dichloro-cis,cis-muconate | pH 7.5, 25°C, wild-type enzyme | Pseudomonas putida | |
432 | - |
2,4-dichloro-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme I54V | Pseudomonas putida | |
600 | - |
2-chloro-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme I54V/F329I | Pseudomonas putida | |
900 | - |
2-chloro-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme Y59W and A271S | Pseudomonas putida | |
1020 | - |
2-chloro-cis,cis-muconate | pH 7.5, 25°C | Acinetobacter calcoaceticus | |
1320 | - |
2,4-dichloro-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme I54V/F329I | Pseudomonas putida | |
1920 | - |
2,4-dichloro-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme F329I | Pseudomonas putida | |
2100 | - |
2-chloro-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme I54V | Pseudomonas putida | |
2880 | - |
2-chloro-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme F329I | Pseudomonas putida | |
2940 | - |
2-chloro-cis,cis-muconate | pH 7.5, 25°C, wild-type enzyme | Pseudomonas putida | |
3120 | - |
3-chloro-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme L333V | Pseudomonas putida | |
3240 | - |
3-methyl-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme L333V | Pseudomonas putida | |
3840 | - |
3-chloro-cis,cis-muconate | pH 7.5, 25°C, wild-type enzyme | Pseudomonas putida | |
4080 | - |
cis,cis-muconate | pH 7.5, 25°C, mutant enzyme L333V | Pseudomonas putida | |
5700 | - |
3-methyl-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme K276N | Pseudomonas putida | |
6600 | - |
3-chloro-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme I54V/F329I | Pseudomonas putida | |
8400 | - |
3-methyl-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme Y59W | Pseudomonas putida | |
12000 | - |
3-chloro-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme I54V | Pseudomonas putida | |
16200 | - |
3-methyl-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme F329I | Pseudomonas putida | |
18600 | - |
3-methyl-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme A271S or I54V/F329 | Pseudomonas putida | |
19200 | - |
2-methyl-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme L333V | Pseudomonas putida | |
22800 | - |
3-chloro-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme K276N | Pseudomonas putida | |
27600 | - |
3-chloro-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme F329I | Pseudomonas putida | |
32400 | - |
cis,cis-muconate | pH 7.5, 25°C, mutant enzyme I54V/F329I | Pseudomonas putida | |
35400 | - |
3-methyl-cis,cis-muconate | pH 7.5, 25°C, wild-type enzyme | Pseudomonas putida | |
39000 | - |
2-methyl-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme I54V | Pseudomonas putida | |
40800 | - |
3-methyl-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme A271S | Pseudomonas putida | |
44400 | - |
3-methyl-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme I54V | Pseudomonas putida | |
57600 | - |
3-chloro-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme A271S | Pseudomonas putida | |
66000 | - |
cis,cis-muconate | pH 7.5, 25°C, mutant enzyme Y59W | Pseudomonas putida | |
72000 | - |
2,4-dichloro-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme I54V | Pseudomonas putida | |
84000 | - |
2-methyl-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme A271S or I54V/F329 | Pseudomonas putida | |
108000 | - |
3-methyl-cis,cis-muconate | pH 7.5, 25°C | Acinetobacter calcoaceticus | |
126000 | - |
cis,cis-muconate | pH 7.5, 25°C, mutant enzyme K276N | Pseudomonas putida | |
150000 | - |
cis,cis-muconate | pH 7.5, 25°C, mutant enzyme I54V | Pseudomonas putida | |
156000 | - |
3-fluoro-cis,cis-muconate | pH 7.5, 25°C | Acinetobacter calcoaceticus | |
156000 | - |
cis,cis-muconate | pH 7.5, 25°C, mutant enzyme F329I | Pseudomonas putida | |
192000 | - |
3-fluoro-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme Y59W | Pseudomonas putida | |
202000 | - |
2-methyl-cis,cis-muconate | pH 7.5, 25°C | Acinetobacter calcoaceticus | |
222000 | - |
cis,cis-muconate | pH 7.5, 25°C | Acinetobacter calcoaceticus | |
222000 | - |
2-methyl-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme Y59W | Pseudomonas putida | |
234000 | - |
2-methyl-cis,cis-muconate | pH 7.5, 25°C, wild-type enzyme | Pseudomonas putida | |
246000 | - |
3-fluoro-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme L333V | Pseudomonas putida | |
294000 | - |
3-fluoro-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme I54V/F329I | Pseudomonas putida | |
304000 | - |
2-methyl-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme A271S | Pseudomonas putida | |
312000 | - |
3-fluoro-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme K276N | Pseudomonas putida | |
360000 | - |
3-fluoro-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme A271S | Pseudomonas putida | |
426000 | - |
cis,cis-muconate | pH 7.5, 25°C, mutant enzyme A271S | Pseudomonas putida | |
480000 | - |
3-fluoro-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme F329I | Pseudomonas putida | |
534000 | - |
3-fluoro-cis,cis-muconate | pH 7.5, 25°C, wild-type enzyme | Pseudomonas putida | |
600000 | - |
2-methyl-cis,cis-muconate | pH 7.5, 25°C, mutant enzyme K276N or F329I | Pseudomonas putida | |
756000 | - |
cis,cis-muconate | pH 7.5, 25°C, wild-type enzyme | Pseudomonas putida |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0035 | - |
2,4-dichloro-cis,cis-muconate | inhibitor of cis,cis-muconate conversion | Pseudomonas putida | |
0.027 | - |
2-fluoro-cis,cis-muconate | inhibitor of cis,cis-muconate conversion | Acinetobacter calcoaceticus | |
0.031 | - |
2-fluoro-cis,cis-muconate | inhibitor of cis,cis-muconate conversion | Pseudomonas putida | |
0.035 | - |
2,4-dichloro-cis,cis-muconate | inhibitor of cis,cis-muconate conversion | Acinetobacter calcoaceticus | |
0.095 | - |
3-chloro-cis,cis-muconate | inhibitor of cis,cis-muconate conversion | Pseudomonas putida |