Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | pressure as a stress condition can significantly increase the activity of intracellular enzyme TSase in Thermus aquaticus. After 2 h of culture under 1.0 MPa pressure, the activity of intracellular TSase in Thermus aquaticus reaches its maximum value. The bacteria are cultured at 70°C, 150 rpm, activity is highest at 1 MPa for 2 h | Thermus aquaticus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltose | Thermus aquaticus | - |
alpha,alpha-trehalose | - |
r | |
maltose | Thermus aquaticus SQ-11 | - |
alpha,alpha-trehalose | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus aquaticus | - |
- |
- |
Thermus aquaticus SQ-11 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme by ammonium sulfate fractionation, dialysis, and two steps of gel filtration, followed by ultrafiltration | Thermus aquaticus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | analysis of enzyme activity at cell growth under pressure of 0.1-1.5 MPa and for 0.5-3 h, overview. For highest enzyme activity, the bacteria are cultured at 70°C, 150 rpm, at 1 MPa for 2 h, in a medium containing 30 g/l potassium glutamate | Thermus aquaticus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltose | - |
Thermus aquaticus | alpha,alpha-trehalose | - |
r | |
maltose | - |
Thermus aquaticus SQ-11 | alpha,alpha-trehalose | - |
r | |
additional information | improvement of activity by reaction condition optimization for trehalose production, overview. Pressure as a stress condition can significantly increase the activity of intracellular enzyme TSase in Thermus aquaticus | Thermus aquaticus | ? | - |
? | |
additional information | improvement of activity by reaction condition optimization for trehalose production, overview. Pressure as a stress condition can significantly increase the activity of intracellular enzyme TSase in Thermus aquaticus | Thermus aquaticus SQ-11 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Trehalose synthase | - |
Thermus aquaticus |
TSase | - |
Thermus aquaticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Thermus aquaticus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the enzyme has a strong thermal stability | Thermus aquaticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Thermus aquaticus |