Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | weak | Cupriavidus necator | |
3-nitrosophenol | autoxidation product of substrate 3-hydroxyaminophenol, inhibition can be partly reversed by addition of 2 mM DTT and/or 10 mM hydroxylamine | Cupriavidus necator | |
AgNO3 | at 1 mM | Cupriavidus necator | |
H2O2 | at 0.0003 mM | Cupriavidus necator | |
L-cysteine | 39% loss of activity after 90 min, 83% loss of activity after 240 min | Cupriavidus necator | |
additional information | not: EDTA, tiron, 10 mM DTT, 0.026 mM NaBH4, 0.1 mM KOCN, 0.1 mM NaN3, Co2+, Cu2+, Fe2+, Fe3+, Mg2+, Mn2+, Ni2+, Zn2+, NaNO2, hydroxylamine | Cupriavidus necator |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
3-hydroxyaminophenol | pH 7.0, on ice | Cupriavidus necator |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | metal cations play no role in the reaction mechanism | Cupriavidus necator |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62000 | - |
x * 62000, SDS-PAGE | Cupriavidus necator |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxyaminophenol | Cupriavidus necator | the enzyme is involved in the degradative pathway of 3-nitrophenol | aminohydroquinone | - |
? | |
3-hydroxyaminophenol | Cupriavidus necator JMP 134-1 | the enzyme is involved in the degradative pathway of 3-nitrophenol | aminohydroquinone | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cupriavidus necator | - |
JMP134 | - |
Cupriavidus necator JMP 134-1 | - |
JMP134 | - |
Purification (Comment) | Organism |
---|---|
- |
Cupriavidus necator |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
3-hydroxyaminophenol = aminohydroquinone | enzyme requires neither a cofactor nor oxygen for the enzymatic reaction, enzyme catalyzes a Bamberg-type rearrangement | Cupriavidus necator |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:3-nitrophenol grown-cell | - |
Cupriavidus necator | - |
culture condition:succinate-grown cell | - |
Cupriavidus necator | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.7 | - |
- |
Cupriavidus necator |
Storage Stability | Organism |
---|---|
-70°C, 7% loss of activity, after 30 days | Cupriavidus necator |
storage on ice, 13% loss of activity after 14 days | Cupriavidus necator |
storage on ice, 37% loss of activity after 38 days | Cupriavidus necator |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-chloro-5-hydroxylaminophenol | - |
Cupriavidus necator | 2-amino-5-chlorohydroquinone | - |
? | |
2-chloro-5-hydroxylaminophenol | - |
Cupriavidus necator JMP 134-1 | 2-amino-5-chlorohydroquinone | - |
? | |
3-hydroxyaminophenol | the enzymatic reaction is regiospecific: the enzyme directs the formation of aminohydroquinone exclusively, whereas the chemical reaction forms the isomeric 4-aminocatechol | Cupriavidus necator | aminohydroquinone | - |
? | |
3-hydroxyaminophenol | the enzyme is involved in the degradative pathway of 3-nitrophenol | Cupriavidus necator | aminohydroquinone | - |
? | |
3-hydroxyaminophenol | the enzymatic reaction is regiospecific: the enzyme directs the formation of aminohydroquinone exclusively, whereas the chemical reaction forms the isomeric 4-aminocatechol | Cupriavidus necator JMP 134-1 | aminohydroquinone | - |
? | |
3-hydroxyaminophenol | the enzyme is involved in the degradative pathway of 3-nitrophenol | Cupriavidus necator JMP 134-1 | aminohydroquinone | - |
? | |
4-hydroxyaminotoluene | - |
Cupriavidus necator | 6-amino-m-cresol | - |
? | |
hydroxylaminobenzene | - |
Cupriavidus necator | 2-aminophenol + 4-aminophenol | - |
? | |
hydroxylaminobenzene | - |
Cupriavidus necator JMP 134-1 | 2-aminophenol + 4-aminophenol | - |
? | |
additional information | not: 4-hydroxylaminobenzoate | Cupriavidus necator | ? | - |
? | |
additional information | not: 4-hydroxylaminobenzoate | Cupriavidus necator JMP 134-1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 62000, SDS-PAGE | Cupriavidus necator |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
1 min: 72% loss of activity, 8 min: complete loss of activity | Cupriavidus necator |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Cupriavidus necator |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Cupriavidus necator | chromatofocusing | - |
4.5 |