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Literature summary for 5.4.4.3 extracted from

  • Schenzle, A.; Lenke, H.; Spain, J.C.; Knackmuss, H.J.
    3-Hydroxylaminophenol mutase from Ralstonia eutropha JMP134 catalyzes a Bamberger rearrangement (1999), J. Bacteriol., 181, 1444-1450.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline weak Cupriavidus necator
3-nitrosophenol autoxidation product of substrate 3-hydroxyaminophenol, inhibition can be partly reversed by addition of 2 mM DTT and/or 10 mM hydroxylamine Cupriavidus necator
AgNO3 at 1 mM Cupriavidus necator
H2O2 at 0.0003 mM Cupriavidus necator
L-cysteine 39% loss of activity after 90 min, 83% loss of activity after 240 min Cupriavidus necator
additional information not: EDTA, tiron, 10 mM DTT, 0.026 mM NaBH4, 0.1 mM KOCN, 0.1 mM NaN3, Co2+, Cu2+, Fe2+, Fe3+, Mg2+, Mn2+, Ni2+, Zn2+, NaNO2, hydroxylamine Cupriavidus necator

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
3-hydroxyaminophenol pH 7.0, on ice Cupriavidus necator

Metals/Ions

Metals/Ions Comment Organism Structure
additional information metal cations play no role in the reaction mechanism Cupriavidus necator

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
x * 62000, SDS-PAGE Cupriavidus necator

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-hydroxyaminophenol Cupriavidus necator the enzyme is involved in the degradative pathway of 3-nitrophenol aminohydroquinone
-
?
3-hydroxyaminophenol Cupriavidus necator JMP 134-1 the enzyme is involved in the degradative pathway of 3-nitrophenol aminohydroquinone
-
?

Organism

Organism UniProt Comment Textmining
Cupriavidus necator
-
JMP134
-
Cupriavidus necator JMP 134-1
-
JMP134
-

Purification (Commentary)

Purification (Comment) Organism
-
Cupriavidus necator

Reaction

Reaction Comment Organism Reaction ID
3-hydroxyaminophenol = aminohydroquinone enzyme requires neither a cofactor nor oxygen for the enzymatic reaction, enzyme catalyzes a Bamberg-type rearrangement Cupriavidus necator

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:3-nitrophenol grown-cell
-
Cupriavidus necator
-
culture condition:succinate-grown cell
-
Cupriavidus necator
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.7
-
-
Cupriavidus necator

Storage Stability

Storage Stability Organism
-70°C, 7% loss of activity, after 30 days Cupriavidus necator
storage on ice, 13% loss of activity after 14 days Cupriavidus necator
storage on ice, 37% loss of activity after 38 days Cupriavidus necator

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-chloro-5-hydroxylaminophenol
-
Cupriavidus necator 2-amino-5-chlorohydroquinone
-
?
2-chloro-5-hydroxylaminophenol
-
Cupriavidus necator JMP 134-1 2-amino-5-chlorohydroquinone
-
?
3-hydroxyaminophenol the enzymatic reaction is regiospecific: the enzyme directs the formation of aminohydroquinone exclusively, whereas the chemical reaction forms the isomeric 4-aminocatechol Cupriavidus necator aminohydroquinone
-
?
3-hydroxyaminophenol the enzyme is involved in the degradative pathway of 3-nitrophenol Cupriavidus necator aminohydroquinone
-
?
3-hydroxyaminophenol the enzymatic reaction is regiospecific: the enzyme directs the formation of aminohydroquinone exclusively, whereas the chemical reaction forms the isomeric 4-aminocatechol Cupriavidus necator JMP 134-1 aminohydroquinone
-
?
3-hydroxyaminophenol the enzyme is involved in the degradative pathway of 3-nitrophenol Cupriavidus necator JMP 134-1 aminohydroquinone
-
?
4-hydroxyaminotoluene
-
Cupriavidus necator 6-amino-m-cresol
-
?
hydroxylaminobenzene
-
Cupriavidus necator 2-aminophenol + 4-aminophenol
-
?
hydroxylaminobenzene
-
Cupriavidus necator JMP 134-1 2-aminophenol + 4-aminophenol
-
?
additional information not: 4-hydroxylaminobenzoate Cupriavidus necator ?
-
?
additional information not: 4-hydroxylaminobenzoate Cupriavidus necator JMP 134-1 ?
-
?

Subunits

Subunits Comment Organism
? x * 62000, SDS-PAGE Cupriavidus necator

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
1 min: 72% loss of activity, 8 min: complete loss of activity Cupriavidus necator

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Cupriavidus necator

pI Value

Organism Comment pI Value Maximum pI Value
Cupriavidus necator chromatofocusing
-
4.5