Information on EC 5.4.4.3 - 3-(hydroxyamino)phenol mutase

for references in articles please use BRENDA:EC5.4.4.3
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The expected taxonomic range for this enzyme is: Cupriavidus necator

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EC NUMBER
COMMENTARY hide
5.4.4.3
-
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RECOMMENDED NAME
GeneOntology No.
3-(hydroxyamino)phenol mutase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3-hydroxyaminophenol = aminohydroquinone
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Aminobenzoate degradation
-
-
Microbial metabolism in diverse environments
-
-
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SYSTEMATIC NAME
IUBMB Comments
3-(hydroxyamino)phenol hydroxymutase
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CAS REGISTRY NUMBER
COMMENTARY hide
224427-05-8
-
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
JMP134
-
-
Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-chloro-5-hydroxylaminophenol
2-amino-5-chlorohydroquinone
show the reaction diagram
3-hydroxyaminophenol
aminohydroquinone
show the reaction diagram
4-hydroxyaminotoluene
6-amino-m-cresol
show the reaction diagram
-
-
-
?
hydroxylaminobenzene
2-aminophenol + 4-aminophenol
show the reaction diagram
additional information
?
-
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3-hydroxyaminophenol
aminohydroquinone
show the reaction diagram
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
metal cations play no role in the reaction mechanism
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
weak
3-nitrosophenol
-
autoxidation product of substrate 3-hydroxyaminophenol, inhibition can be partly reversed by addition of 2 mM DTT and/or 10 mM hydroxylamine
AgNO3
-
at 1 mM
H2O2
-
at 0.0003 mM
L-cysteine
-
39% loss of activity after 90 min, 83% loss of activity after 240 min
additional information
-
not: EDTA, tiron, 10 mM DTT, 0.026 mM NaBH4, 0.1 mM KOCN, 0.1 mM NaN3, Co2+, Cu2+, Fe2+, Fe3+, Mg2+, Mn2+, Ni2+, Zn2+, NaNO2, hydroxylamine
-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.1
3-hydroxyaminophenol
-
pH 7.0, on ice
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5
-
chromatofocusing
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
62000
-
x * 62000, SDS-PAGE
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60
-
1 min: 72% loss of activity, 8 min: complete loss of activity
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-70°C, 7% loss of activity, after 30 days
-
storage on ice, 13% loss of activity after 14 days
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storage on ice, 37% loss of activity after 38 days
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
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LINKS TO OTHER DATABASES (specific for EC-Number 5.4.4.3)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)