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Literature summary for 5.4.2.8 extracted from

  • Pirard, M.; Achouri, Y.; Collet, J.F.; Schollen, E.; Matthijs, G.; Van Schaftingen, E.
    Kinetic properties and tissular distribution of mammalian phosphomannomutase isozymes (1999), Biochem. J., 339, 201-207.
No PubMed abstract available
Search for more literature for 5.4.2.8

Activating Compound

Activating Compound Comment Organism Structure
D-Glucose 1,6-bisphosphate PMM2 requires a hexose bisphosphate for activity, as potent as mannose 1,6-bisphosphate in activation Homo sapiens
D-Mannose 1,6-bisphosphate PMM2 requires a hexose bisphosphate for activity, as potent as glucose 1,6-bisphosphate in activation, half-maximal activation in the presence of 0.01 mM and 0.1 mM at 0.0005 mM and 0.001 mM mannose 1,6-bisphosphate, respectively Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
vanadate 0.05 mM, 50% inhibition Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
 alpha-D-glucose 1-phosphate
-
 Homo sapiens
0.018
-
 D-mannose 1-phosphate
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alpha-D-mannose 1-phosphate Rattus norvegicus 20fold higher activity than with alpha-D-glucose 1-phosphate alpha-D-mannose 6-phosphate
-
 r
alpha-D-mannose 1-phosphate Homo sapiens
-
 alpha-D-mannose 6-phosphate
-
 r

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant PMM2, polyethylene glycol, DEAE-Sepharose, Q-Sepharose Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
49
-
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-glucose 1-phosphate
-
 Homo sapiens alpha-D-glucose 6-phosphate
-
 r
alpha-D-glucose 1-phosphate
-
 Homo sapiens D-glucose 6-phosphate
-
 ?
alpha-D-mannose 1-phosphate 20fold higher activity than with alpha-D-glucose 1-phosphate Homo sapiens alpha-D-mannose 6-phosphate
-
 r
alpha-D-mannose 1-phosphate 20fold higher activity than with alpha-D-glucose 1-phosphate Rattus norvegicus alpha-D-mannose 6-phosphate
-
 r
alpha-D-mannose 1-phosphate
-
 Homo sapiens alpha-D-mannose 6-phosphate
-
 r

Synonyms

Synonyms Comment Organism
PMM2
-
 Homo sapiens
PMM2
-
 Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
7.1
-
 80% of maximal activity Homo sapiens