Cloned (Comment) | Organism |
---|---|
gene PAGM, DNA and amino acid sequence determination and analysis, expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Trypanosoma brucei |
Protein Variants | Comment | Organism |
---|---|---|
additional information | RNAi knockdown of gene TbPAGM, construction of a cell line expressing tetracycline inducible double-stranded RNA targeting TbPMM, induction of dsRNA targeting TbPMM results in a 68% knockdown of TbPMM mRNA after 48 h and a reduction of growth rate that leads to cell death after 72 h, phenotypes, overview | Trypanosoma brucei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-glucose 1-phosphate | competitive inhibition | Trypanosoma brucei | |
D-glucose 6-phosphate | competitive inhibition | Trypanosoma brucei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
N-acetyl-D-glucosamine 6-phosphate | pH 7.3, 37°C | Trypanosoma brucei |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Trypanosoma brucei | 5829 | - |
glycosome | - |
Trypanosoma brucei | 20015 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Trypanosoma brucei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Trypanosoma brucei | the enzyme exhibits the function of phosphoglucomutase, EC 5.4.2.2, whose gene is lost in Trypanosoma brucei, together with phosphomannomutase, EC 5.4.2.8, producing D-glucose 1-phosphate from D-glucose 6-phosphate, kinetics, overview | ? | - |
? | |
additional information | Trypanosoma brucei 427 | the enzyme exhibits the function of phosphoglucomutase, EC 5.4.2.2, whose gene is lost in Trypanosoma brucei, together with phosphomannomutase, EC 5.4.2.8, producing D-glucose 1-phosphate from D-glucose 6-phosphate, kinetics, overview | ? | - |
? | |
N-acetyl-alpha-D-glucosamine 1-phosphate | Trypanosoma brucei | - |
N-acetyl-D-glucosamine 6-phosphate | - |
r | |
N-acetyl-alpha-D-glucosamine 1-phosphate | Trypanosoma brucei 427 | - |
N-acetyl-D-glucosamine 6-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | F4NCC3 | gene PAGM | - |
Trypanosoma brucei 427 | F4NCC3 | gene PAGM | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Trypanosoma brucei |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | quantitative proteomic analysis show that the TbPAGM protein is present in both life cycle stages, procyclic and bloodstream forms | Trypanosoma brucei | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme exhibits the function of phosphoglucomutase, EC 5.4.2.2, whose gene is lost in Trypanosoma brucei, together with phosphomannomutase, EC 5.4.2.8, producing D-glucose 1-phosphate from D-glucose 6-phosphate, kinetics, overview | Trypanosoma brucei | ? | - |
? | |
additional information | the enzyme exhibits the function of phosphoglucomutase, EC 5.4.2.2, whose gene is lost in Trypanosoma brucei, together with phosphomannomutase, EC 5.4.2.8, producing D-glucose 1-phosphate from D-glucose 6-phosphate, kinetics, overview | Trypanosoma brucei 427 | ? | - |
? | |
N-acetyl-alpha-D-glucosamine 1-phosphate | - |
Trypanosoma brucei | N-acetyl-D-glucosamine 6-phosphate | - |
r | |
N-acetyl-alpha-D-glucosamine 1-phosphate | - |
Trypanosoma brucei 427 | N-acetyl-D-glucosamine 6-phosphate | - |
r | |
N-acetyl-D-glucosamine 6-phosphate | - |
Trypanosoma brucei | N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r | |
N-acetyl-D-glucosamine 6-phosphate | - |
Trypanosoma brucei 427 | N-acetyl-alpha-D-glucosamine 1-phosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
PAGM | - |
Trypanosoma brucei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Trypanosoma brucei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | 9.1 | broad optimum | Trypanosoma brucei |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0063 | - |
versus N-acetyl-D-glucosamine 6-phosphate, pH 7.3, 37°C | Trypanosoma brucei | D-glucose 1-phosphate | |
0.0083 | - |
versus N-acetyl-D-glucosamine 6-phosphate, pH 7.3, 37°C | Trypanosoma brucei | D-glucose 6-phosphate |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the alpha-D-phosphohexomutase superfamily of enzymes | Trypanosoma brucei |
physiological function | the enzyme is essential for parasite growth. It takes over the function of phosphoglucomutase, EC 5.4.2.2, whose gene is lost in Trypanosoma brucei, together with phosphomannomutase, EC 5.4.2.8, producing D-glucose 1-phosphate from D-glucose 6-phosphate, kinetics, overview | Trypanosoma brucei |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
16 | - |
N-acetyl-D-glucosamine 6-phosphate | pH 7.3, 37°C | Trypanosoma brucei |