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Information on EC 5.4.2.3 - phosphoacetylglucosamine mutase
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EC Tree
5.4.2.3 phosphoacetylglucosamine mutaseIUBMB Comments
The enzyme is activated by N-acetyl-alpha-D-glucosamine 1,6-bisphosphate.
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Word Map
- 5.4.2.3
- udp-n-acetylglucosamine
- phosphoglucomutase
-
pyrophosphorylase
- mutases
- udp-glcnac
-
glcnac-6-p
-
phosphoglucosamine
- phosphoserine
- synthesis
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Reaction Schemes
Synonyms
phosphoacetylglucosamine mutase, n-acetylglucosamine-phosphate mutase, phospho-n-acetylglucosamine mutase, hsagm1, caagm1, acetylglucosamine phosphomutase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
2-Acetamido-2-deoxy-D-glucose-1,6-bisphosphate:2-acetamido-2-deoxy-D-glucose 1-phosphate phosphotransferase
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-
-
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Acetylaminodeoxyglucose phosphomutase
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Acetylglucosamine phosphomutase
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-
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DNA-damage-repair/toleration protein DRT101
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-
-
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EC 2.7.5.2
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formerly
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EC 2.7.5.2.
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formerly
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N-acetylglucosamine-phosphate mutase
PAGM
PGlcNAc mutase
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-
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Phospho-N-acetylglucosamine mutase
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-
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Phosphomutase, acetylglucosamine
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-
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N-acetylglucosamine-phosphate mutase
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-
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PAGM
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-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
N-acetyl-alpha-D-glucosamine 1-phosphate = N-acetyl-D-glucosamine 6-phosphate
ping-pong mechanism
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N-acetyl-alpha-D-glucosamine 1-phosphate = N-acetyl-D-glucosamine 6-phosphate
the N-acetyl group of the substrate is recognized by V370 and N389 in domain 3, from which the substrate specificity arises. Substrate rotates about 180° on the axis linking C4 and the midpoint of the C5-O5 bond in the reaction
N-acetyl-alpha-D-glucosamine 1-phosphate = N-acetyl-D-glucosamine 6-phosphate
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
group transfer
-
-
intramolecular, phosphate group
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isomerization
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-
-
-
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
N-Acetyl-alpha-D-glucosamine 1,6-phosphomutase
The enzyme is activated by N-acetyl-alpha-D-glucosamine 1,6-bisphosphate.
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CAS REGISTRY NUMBER
COMMENTARY
9027-51-4
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
N-acetyl-alpha-D-glucosamine 6-phosphate
N-acetyl-alpha-D-glucosamine 1-phosphate
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-
-
-
r
Glucose 1-phosphate
Glucose 6-phosphate
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in presence of glucose 1,6-diphosphate
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?
N-acetyl-alpha-D-glucosamine 1-phosphate
N-acetyl-alpha-D-glucosamine 6-phosphate
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-
-
-
r
N-acetyl-alpha-D-glucosamine 1-phosphate
N-acetyl-alpha-D-glucosamine 6-phosphate
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-
-
r
N-acetyl-alpha-D-glucosamine 1-phosphate
N-acetyl-D-glucosamine 6-phosphate
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-
?
N-acetyl-alpha-D-glucosamine 1-phosphate
N-acetyl-D-glucosamine 6-phosphate
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-
-
-
?
N-acetyl-alpha-D-glucosamine 1-phosphate
N-acetyl-D-glucosamine 6-phosphate
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-
-
-
?
N-acetyl-alpha-D-glucosamine 1-phosphate
N-acetyl-D-glucosamine 6-phosphate
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-
?
N-acetyl-alpha-D-glucosamine 1-phosphate
N-acetyl-D-glucosamine 6-phosphate
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-
-
r
N-acetyl-alpha-D-glucosamine 1-phosphate
N-acetyl-D-glucosamine 6-phosphate
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-
-
r
N-Acetyl-D-glucosamine 1-phosphate
N-Acetyl-D-glucosamine 6-phosphate
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-
-
-
?
N-Acetyl-D-glucosamine 1-phosphate
N-Acetyl-D-glucosamine 6-phosphate
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specific for the alpha-1-phosphate anomer
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-
?
N-Acetyl-D-glucosamine 1-phosphate
N-Acetyl-D-glucosamine 6-phosphate
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-
-
-
?
N-Acetyl-D-glucosamine 1-phosphate
N-Acetyl-D-glucosamine 6-phosphate
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in presence of N-acetylglucosamine 1,6-diphosphate
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?
N-acetyl-D-glucosamine 6-phosphate
N-acetyl-alpha-D-glucosamine 1-phosphate
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-
-
r
N-acetyl-D-glucosamine 6-phosphate
N-acetyl-alpha-D-glucosamine 1-phosphate
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-
-
r
N-acetyl-D-glucosamine 6-phosphate
N-acetyl-alpha-D-glucosamine 1-phosphate
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-
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r
N-Acetylglucosamine 6-phosphate
?
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the reaction is involved in the synthesis of active glycosyl intermediates in reticulo-endothelial tissues
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-
?
N-Acetylglucosamine 6-phosphate
?
Coffea sp.
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enzyme in the pathway of UDP-N-acetyl-D-glucosamine formation
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-
?
N-Acetylglucosamine 6-phosphate
?
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enzyme is involved in galactosamine synthesis from glucose during encystment, induced during encystment
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-
?
N-Acetylglucosamine 6-phosphate
?
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enzyme is involved in galactosamine synthesis from glucose during encystment, induced during encystment
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-
?
N-Acetylglucosamine 6-phosphate
N-Acetylglucosamine 1-phosphate
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-
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?
additional information
?
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one of the two last pathway-specific enzymes of hexosamine biosynthesis. The hexosamine pathway is regulated at the post-translational level during zoospore germination
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-
?
additional information
?
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the enzyme exhibits the function of phosphoglucomutase, EC 5.4.2.2, whose gene is lost in Trypanosoma brucei, together with phosphomannomutase, EC 5.4.2.8, producing D-glucose 1-phosphate from D-glucose 6-phosphate, kinetics, overview
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-
?
additional information
?
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the enzyme exhibits the function of phosphoglucomutase, EC 5.4.2.2, whose gene is lost in Trypanosoma brucei, together with phosphomannomutase, EC 5.4.2.8, producing D-glucose 1-phosphate from D-glucose 6-phosphate, kinetics, overview
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-
?
additional information
?
-
the enzyme exhibits the function of phosphoglucomutase, EC 5.4.2.2, whose gene is lost in Trypanosoma brucei, together with phosphomannomutase, EC 5.4.2.8, producing D-glucose 1-phosphate from D-glucose 6-phosphate, kinetics, overview
-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
N-acetyl-alpha-D-glucosamine 6-phosphate
N-acetyl-alpha-D-glucosamine 1-phosphate
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-
-
-
r
N-acetyl-D-glucosamine 6-phosphate
N-acetyl-alpha-D-glucosamine 1-phosphate
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-
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r
N-acetyl-alpha-D-glucosamine 1-phosphate
N-acetyl-alpha-D-glucosamine 6-phosphate
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-
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r
N-acetyl-alpha-D-glucosamine 1-phosphate
N-acetyl-alpha-D-glucosamine 6-phosphate
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-
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r
N-acetyl-alpha-D-glucosamine 1-phosphate
N-acetyl-D-glucosamine 6-phosphate
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-
?
N-acetyl-alpha-D-glucosamine 1-phosphate
N-acetyl-D-glucosamine 6-phosphate
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-
?
N-acetyl-alpha-D-glucosamine 1-phosphate
N-acetyl-D-glucosamine 6-phosphate
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-
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r
N-acetyl-alpha-D-glucosamine 1-phosphate
N-acetyl-D-glucosamine 6-phosphate
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-
r
N-Acetylglucosamine 6-phosphate
?
-
the reaction is involved in the synthesis of active glycosyl intermediates in reticulo-endothelial tissues
-
-
?
N-Acetylglucosamine 6-phosphate
?
Coffea sp.
-
enzyme in the pathway of UDP-N-acetyl-D-glucosamine formation
-
-
?
N-Acetylglucosamine 6-phosphate
?
-
enzyme is involved in galactosamine synthesis from glucose during encystment, induced during encystment
-
-
?
N-Acetylglucosamine 6-phosphate
?
-
enzyme is involved in galactosamine synthesis from glucose during encystment, induced during encystment
-
-
?
additional information
?
-
-
one of the two last pathway-specific enzymes of hexosamine biosynthesis. The hexosamine pathway is regulated at the post-translational level during zoospore germination
-
-
?
additional information
?
-
the enzyme exhibits the function of phosphoglucomutase, EC 5.4.2.2, whose gene is lost in Trypanosoma brucei, together with phosphomannomutase, EC 5.4.2.8, producing D-glucose 1-phosphate from D-glucose 6-phosphate, kinetics, overview
-
-
?
additional information
?
-
-
the enzyme exhibits the function of phosphoglucomutase, EC 5.4.2.2, whose gene is lost in Trypanosoma brucei, together with phosphomannomutase, EC 5.4.2.8, producing D-glucose 1-phosphate from D-glucose 6-phosphate, kinetics, overview
-
-
?
additional information
?
-
the enzyme exhibits the function of phosphoglucomutase, EC 5.4.2.2, whose gene is lost in Trypanosoma brucei, together with phosphomannomutase, EC 5.4.2.8, producing D-glucose 1-phosphate from D-glucose 6-phosphate, kinetics, overview
-
-
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mg2+
phosphate
-
covalently linked to the enzyme. The phosphate-protein linkage is stable in mild acid, at 50 C for up to 2 h. However phosphate is completely released under mild alkaline conditions at 50 C within 1 h
Mg2+
-
divalent metal ion required. Optimal Mg2+ concentration in the presence of dithiothreitol is 10 mM, maximal activity with other metal chelators is obtained with 1 mM Mg2+
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
(3aR,5R,6S,7R,7aR)-5-(hydroxymethyl)-2-methyl-5,6,7,7a-tetrahydro-3aH-pyrano[3,2-d][1,3]oxazole-6,7-diol
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2,5:4,8-dianhydro-1,3-dideoxy-9-O-phosphono-D-erythro-L-gulo-nonitol
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2-acetamido-1,5-anhydro-2,6,7-trideoxy-7-phosphono-D-gluco-heptitol
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2-acetamido-2-deoxy-N-phosphono-6-O-phosphono-D-glucopyranosylamine
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[(3aR,5R,6S,7R,7aR)-6,7-dihydroxy-2-methyl-5,6,7,7a-tetrahydro-3aH-pyrano[3,2-d][1,3]oxazol-5-yl]methyl phosphate
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
N-Acetylglucosamine 1,6-diphosphate
-
required for reaction with N-acetylglucosamine 1-phosphate
glucose 1,6-diphosphate
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required, maximal activity at 0.005-0.05 mM
glucose 1,6-diphosphate
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required for reaction with glucose 1-phosphate, Km: 0.025 mM
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0063
D-glucose 1-phosphate
Trypanosoma brucei
versus N-acetyl-D-glucosamine 6-phosphate, pH 7.3, 37°C
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6.5 - 9
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pH 6.5: about 45% of maximal activity, pH 9.0: about 40% of maximal activity, Tris-maleate buffer
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
additional information
additional information
quantitative proteomic analysis show that the TbPAGM protein is present in both life cycle stages, procyclic and bloodstream forms
brenda
additional information
-
quantitative proteomic analysis show that the TbPAGM protein is present in both life cycle stages, procyclic and bloodstream forms
brenda
additional information
-
quantitative proteomic analysis show that the TbPAGM protein is present in both life cycle stages, procyclic and bloodstream forms
-
brenda
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
evolution
malfunction
loss-of-function mutations in the nst gene, which encodes PGM3, block fibroblast growth factor signaling during embryonic mesoderm and tracheal development. In nst mutants with an 80% reduction in the amount of UDP-GlcNAc, O-GlcNAcylation is compromised. Embryos maternally and zygotically mutant for nst exhibit defects in mesoderm layer formation during gastrulation that are similar to those observed in embryos with mutations in the gene encoding the fibroblast growth factor receptor Heartless (Htl). Zygotic nst mutants (nstZ) exhibit normal dorsal mesoderm differentiation, embryos homozygous for the hypomorphic htlYY262 allele show a reduction in dorsal mesoderm. Phenotypes, overview. Dof is the critical component in MAPK activation affected in nst mutants
metabolism
Nst acts downstream of fibroblast growth factor receptor signaling
physiological function
evolution
the enzyme belongs to the alpha-D-phosphohexomutase superfamily of enzymes
evolution
-
the enzyme belongs to the alpha-D-phosphohexomutase superfamily of enzymes
-
physiological function
the enzyme is essential for parasite growth. It takes over the function of phosphoglucomutase, EC 5.4.2.2, whose gene is lost in Trypanosoma brucei, together with phosphomannomutase, EC 5.4.2.8, producing D-glucose 1-phosphate from D-glucose 6-phosphate, kinetics, overview
physiological function
the enzyme is required for normal cellular levels of UDP-GlcNAc and essential in UDP-GlcNAc biosynthesis, and for fibroblast growth factor signaling in early embryos. Role for Nst in fibroblast growth factor receptor Heartless (Htl) signaling
physiological function
-
the enzyme is essential for parasite growth. It takes over the function of phosphoglucomutase, EC 5.4.2.2, whose gene is lost in Trypanosoma brucei, together with phosphomannomutase, EC 5.4.2.8, producing D-glucose 1-phosphate from D-glucose 6-phosphate, kinetics, overview
-
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PDB
SCOP
CATH
UNIPROT
ORGANISM
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE