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Literature summary for 5.3.99.12 extracted from

  • Masamura, N.; Ohashi, W.; Tsuge, N.; Imai, S.; Ishii-Nakamura, A.; Hirota, H.; Nagata, T.; Kumagai, H.
    Identification of amino acid residues essential for onion lachrymatory factor synthase activity (2012), Biosci. Biotechnol. Biochem., 76, 447-453 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL-21-Gold cells Allium cepa

Protein Variants

Protein Variants Comment Organism
C69S/R71L/K74L inactive Allium cepa
E88Q inactive Allium cepa
E88Q/D94N inactive Allium cepa
R71L inactive Allium cepa
T109A/E112Q/Y114F the mutant shows very low activity compared to the wild type enzyme Allium cepa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-propenylsulfenic acid Allium cepa
-
propanethial S-oxide
-
?

Organism

Organism UniProt Comment Textmining
Allium cepa P59082
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-propenylsulfenic acid
-
Allium cepa propanethial S-oxide
-
?

Synonyms

Synonyms Comment Organism
lachrymatory factor synthase
-
Allium cepa
LFS
-
Allium cepa