Literature summary for 5.3.99.12 extracted from

Silvaroli, J.A.; Pleshinger, M.J.; Banerjee, S.; Kiser, P.D.; Golczak, M.
Enzyme that makes you cry-crystal structure of lachrymatory factor synthase from Allium cepa (2017), ACS Chem. Biol., 12, 2296-2304 .

Search for more literature for 5.3.99.12

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Allium cepa

Crystallization (Commentary)

Crystallization (Comment)	Organism
apo-form and in complex with crotyl alcohol, sitting drop vapor diffusion method, using 0.1 M sodium acetate at pH 4.5 containing PEG 3350 at concentrations between 25% and 32% (w/v)	Allium cepa

Protein Variants

Protein Variants	Comment	Organism
R71L/E88Q	the mutations completely abolish the enzymatic reaction	Allium cepa

Inhibitors

Inhibitors	Comment	Organism	Structure
crotyl alcohol	i.e. (2E)-but-2-en-1-ol	Allium cepa

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(E)-prop-1-en-1-SO-peroxol	Allium cepa	-	(Z)-propanethial S-oxide	-	?

Organism

Organism	UniProt	Comment	Textmining
Allium cepa	P59082	-	-

Purification (Commentary)

Purification (Comment)	Organism
glutathione-Sepharose resin column chromatography, Superdex 200 gel filtration, and HiTrap Q ion exchange column chromatography	Allium cepa

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(E)-prop-1-en-1-SO-peroxol	-	Allium cepa	(Z)-propanethial S-oxide	-	?

Synonyms

Synonyms	Comment	Organism
lachrymatory factor synthase	-	Allium cepa
LFS	-	Allium cepa

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Release 2023.1 (January 2023)