Cloned (Comment) | Organism |
---|---|
expression of His-tagged wild-type and mutant PDIs in Escherichia coli strain M15 | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
small angle X-ray scattering analysis of purified recombinant wild-type and deletion mutant PDIs, structure modeling, the enzyme forms an approximately flat elliptical cylinder | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of several deletion mutants of PDI by elimination of partial domains or whole domains, overview | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
64000 | - |
1 * 64000, analytical ultracentrifugation | Homo sapiens |
69000 | - |
light scattering | Homo sapiens |
116000 | - |
gel filtration, the C-terminal fragment comprising residues 441-491 contributes to the anomalous molecular mass determination by gel filtration | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P07237 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant PDIs from Escherichia coli strain M15 by nickel affinity and cation exchange chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the four domains a, b, b', and a' show cooperative properties in both isomerase and chaperone functions of PDi | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 64000, analytical ultracentrifugation | Homo sapiens |
More | the four domains a, b, b', and a' are arranged in an annular manner | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
PDI | - |
Homo sapiens |