Any feedback?
Literature summary for 4.98.1.1 extracted from
- Ferrochelatase catalyzes the formation of Zn-protoporphyrin of dry-cured ham via the conversion reaction from heme in meat (2011), J. Agric. Food Chem., 59, 12238-12245.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ascorbate | optimum activity at 6 mM ascorbate | Thermus thermophilus |  |
| ascorbate | optimum activity at 6 mM ascorbate | Sus scrofa | |
| ascorbate | optimum activity at 6 mM ascorbate | Saccharomyces cerevisiae | |
| L-cysteine | optimum activity at 6 mM L-cysteine | Thermus thermophilus | |
| L-cysteine | optimum activity at 6 mM L-cysteine | Sus scrofa | |
| L-cysteine | optimum activity at 6 mM L-cysteine | Saccharomyces cerevisiae | |
| NaCl | optimum activity at 100 mM NaCl | Thermus thermophilus | |
| NaCl | optimum activity at 100 mM NaCl | Sus scrofa | |
| NaCl | optimum activity at 100 mM NaCl | Saccharomyces cerevisiae | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Saccharomyces cerevisiae |
| expressed in Escherichia coli BL21(DE3) cells | Sus scrofa |
| expressed in Escherichia coli BL21(DE3) cells | Thermus thermophilus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.012 | - |
myoglobin | at pH 6.5 and 30°C | Saccharomyces cerevisiae | |
| 0.06 | - |
Zn2+ | at pH 6.5 and 30°C | Saccharomyces cerevisiae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrial inner membrane | - |
Sus scrofa | 5743 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
x * 33000, SDS-PAGE | Thermus thermophilus |
| 39000 | - |
x * 39000, SDS-PAGE | Saccharomyces cerevisiae |
| 42000 | - |
x * 42000, SDS-PAGE | Sus scrofa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| protoporphyrin IX + Fe2+ | Thermus thermophilus | - |
protoheme IX + 2 H+ | - |
? | |
| protoporphyrin IX + Fe2+ | Sus scrofa | - |
protoheme IX + 2 H+ | - |
? | |
| protoporphyrin IX + Fe2+ | Saccharomyces cerevisiae | - |
protoheme IX + 2 H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
| Sus scrofa | - |
- |
- |
| Thermus thermophilus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni2+-NTA bead chromatography | Saccharomyces cerevisiae |
| Ni2+-NTA bead chromatography | Sus scrofa |
| Ni2+-NTA bead chromatography | Thermus thermophilus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| muscle | - |
Sus scrofa | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 18°C, in 20 mM Tris-HCl (pH 8.0) containing 0.3 M NaCl, about 2 weeks, 42% residual activity | Saccharomyces cerevisiae |
| 4°C, in 20 mM Tris-HCl (pH 8.0) containing 0.3 M NaCl, 1 month, no loss of activity | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hemoglobin + Zn2+ | - |
Thermus thermophilus | Zn-protoporphyrin + H+ | - |
? | |
| hemoglobin + Zn2+ | - |
Sus scrofa | Zn-protoporphyrin + H+ | - |
? | |
| hemoglobin + Zn2+ | - |
Saccharomyces cerevisiae | Zn-protoporphyrin + H+ | - |
? | |
| mesoporphyrin IX + Fe2+ | - |
Thermus thermophilus | ? | - |
? | |
| mesoporphyrin IX + Fe2+ | - |
Sus scrofa | ? | - |
? | |
| mesoporphyrin IX + Fe2+ | - |
Saccharomyces cerevisiae | ? | - |
? | |
| myoglobin + Zn2+ | - |
Thermus thermophilus | Zn-protoporphyrin + H+ | - |
? | |
| myoglobin + Zn2+ | - |
Sus scrofa | Zn-protoporphyrin + H+ | - |
? | |
| myoglobin + Zn2+ | - |
Saccharomyces cerevisiae | Zn-protoporphyrin + H+ | - |
? | |
| protoporphyrin IX + Fe2+ | - |
Thermus thermophilus | protoheme IX + 2 H+ | - |
? | |
| protoporphyrin IX + Fe2+ | - |
Sus scrofa | protoheme IX + 2 H+ | - |
? | |
| protoporphyrin IX + Fe2+ | - |
Saccharomyces cerevisiae | protoheme IX + 2 H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 33000, SDS-PAGE | Thermus thermophilus |
| ? | x * 42000, SDS-PAGE | Sus scrofa |
| ? | x * 39000, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FeCH | - |
Thermus thermophilus |
| FeCH | - |
Sus scrofa |
| FeCH | - |
Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
reverse reaction | Thermus thermophilus |
| 5.5 | - |
reverse reaction | Sus scrofa |
| 5.5 | - |
reverse reaction | Saccharomyces cerevisiae |
| 7.5 | 8 | forward reaction | Thermus thermophilus |
| 7.5 | 8 | forward reaction | Sus scrofa |
| 7.5 | 8 | forward reaction | Saccharomyces cerevisiae |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2026.1 (March 2026)
Legal
Curated at
Member of
![DSMZ Digital Diversity]()
![Global Core Biodata Resource]()
![ELIXIR Core Data Resource]()
![German Network for Bioinformatics Infrastructure]()
