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Literature summary for 4.98.1.1 extracted from

  • Hansson, M.D.; Karlberg, T.; Soederberg, C.A.; Rajan, S.; Warren, M.J.; Al-Karadaghi, S.; Rigby, S.E.; Hansson, M.
    Bacterial ferrochelatase turns human: Tyr13 determines the apparent metal specificity of Bacillus subtilis ferrochelatase (2011), J. Biol. Inorg. Chem., 16, 235-242.
    View publication on PubMed
Search for more literature for 4.98.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Bacillus subtilis
expression in Escherichia coli BL21(DE3) Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
Y13M the mutant enzyme inserts Co2+ at a higher rate than the wild type ferrochelatase, but it has lost the ability to use Cu2+ as a substrate Bacillus subtilis
Y13M the modified enzyme inserts Co2+ at a higher rate than the wild-type enzyme but loses the ability to use Cu2+ as a substrate. the mutant enzyme shows the same metal specificity as that of the ferrochelatases from Saccharomyces cerevisiae and human Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0052
-
 Co2+ wild type enzyme, in 100 mM tris(hydroxymethyl)aminomethane-HCl, pH 7.4, temperature not specified in the publication Bacillus subtilis
0.0052
-
 Co2+ pH 7.4, 21°C, wild-type enzyme Bacillus subtilis
0.013
-
 Zn2+ pH 7.4, 21°C, wild-type enzyme Bacillus subtilis
0.063
-
 Cu2+ wild type enzyme, in 100 mM tris(hydroxymethyl)aminomethane-HCl, pH 7.4, temperature not specified in the publication Bacillus subtilis
0.063
-
 Cu2+ pH 7.4, 21°C, wild-type enzyme Bacillus subtilis
0.56
-
 Co2+ mutant enzyme Y13M, in 100 mM tris(hydroxymethyl)aminomethane-HCl, pH 7.4, temperature not specified in the publication Bacillus subtilis
0.56
-
 Co2+ pH 7.4, 21°C, mutant enzyme Y13M Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
protoporphyrin IX + Fe2+ Bacillus subtilis
-
 protoheme IX + 2 H+
-
 ?
protoporphyrin IX + Fe2+ Bacillus subtilis the enzymatic product protoheme IX is a well-known cofactor in a wide range of proteins protoheme IX + 2 H+
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis P32396
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme is promiscuous in vitro and can insert Zn2+, Co2+, Cu2+, and Ni2+ into protoporphyrin IX in addition to the physiological substrate Fe2+, however, the insertion of metal ions other than Fe2+ occurs rarely in vivo Bacillus subtilis ?
-
 ?
protoporphyrin IX + Co2+
-
 Bacillus subtilis Co2+-protoporphyrin + H+
-
 ?
protoporphyrin IX + Co2+
-
 Bacillus subtilis ?
-
 ?
protoporphyrin IX + Cu2+ Cu2+ is a good substrate Bacillus subtilis Cu2+-protoporphyrin + H+
-
 ?
protoporphyrin IX + Cu2+
-
 Bacillus subtilis ?
-
 ?
protoporphyrin IX + Fe2+
-
 Bacillus subtilis protoheme IX + 2 H+
-
 ?
protoporphyrin IX + Fe2+ the enzymatic product protoheme IX is a well-known cofactor in a wide range of proteins Bacillus subtilis protoheme IX + 2 H+
-
 ?
protoporphyrin IX + Zn2+
-
 Bacillus subtilis ?
-
 ?

Synonyms

Synonyms Comment Organism
hemH
-
 Bacillus subtilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.016
-
 Co2+ wild type enzyme, in 100 mM tris(hydroxymethyl)aminomethane-HCl, pH 7.4, temperature not specified in the publication Bacillus subtilis
0.016
-
 Co2+ pH 7.4, 21°C, wild-type enzyme Bacillus subtilis
0.033
-
 Co2+ mutant enzyme Y13M, in 100 mM tris(hydroxymethyl)aminomethane-HCl, pH 7.4, temperature not specified in the publication Bacillus subtilis
0.033
-
 Co2+ pH 7.4, 21°C, mutant enzyme Y13M Bacillus subtilis
0.065
-
 Cu2+ wild type enzyme, in 100 mM tris(hydroxymethyl)aminomethane-HCl, pH 7.4, temperature not specified in the publication Bacillus subtilis
0.065
-
 Cu2+ pH 7.4, 21°C, wild-type enzyme Bacillus subtilis

General Information

General Information Comment Organism
metabolism the enzymatic product protoheme IX is a well-known cofactor in a wide range of proteins Bacillus subtilis