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Literature summary for 4.98.1.1 extracted from
- Probing the active site loop motif of murine ferrochelatase by random mutagenesis (2004), J. Biol. Chem., 279, 19977-19986.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Mus musculus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| K250M/V251L/W256Y | higher KM value than the wild-type with protoporphyrin IX as substrate, lower KM-value than the wild-type with Fe2+ as the substrate | Mus musculus |
| P255R | higher KM value than the wild-type with protoporphyrin IX as substrate, lower KM-value than the wild-type with Fe2+ as the substrate | Mus musculus |
| Q248P/S249G/K250P/G252W | higher KM value than the wild-type with protoporphyrin IX as substrate, lower KM-value than the wild-type with Fe2+ as the substrate | Mus musculus |
| S249A/K250Q/V251C | the ratio of iron/protein is 50% higher than that of the wild-type enzyme, higher KM-values than the wild-type | Mus musculus |
| V251L | higher KM value than the wild-type with protoporphyrin IX as substrate, lower KM-value than the wild-type with Fe2+ as the substrate | Mus musculus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00048 | - |
Fe2+ | mutant Q248P/S249G/K250P/G252W, pH 8.1, 30°C | Mus musculus |  |
| 0.00108 | - |
Fe2+ | mutant K250M/V251L/W256Y, pH 8.1, 30°C | Mus musculus | |
| 0.00113 | - |
Fe2+ | mutant V251L, pH 8.1, 30°C | Mus musculus | |
| 0.0014 | - |
protoporphyrin IX | wild-type, pH 8.1, 30°C | Mus musculus | |
| 0.00146 | - |
Fe2+ | mutant P255R, pH 8.1, 30°C | Mus musculus | |
| 0.00182 | - |
protoporphyrin IX | mutant V251L, pH 8.1, 30°C | Mus musculus | |
| 0.0019 | - |
Fe2+ | wild-type, pH 8.1, 30°C | Mus musculus | |
| 0.00265 | - |
protoporphyrin IX | mutant P255R, pH 8.1, 30°C | Mus musculus | |
| 0.00515 | - |
Fe2+ | mutant S249A/K250Q/V251C, pH 8.1, 30°C | Mus musculus | |
| 0.00684 | - |
protoporphyrin IX | mutant S249A/K250Q/V251C, pH 8.1, 30°C | Mus musculus | |
| 0.01034 | - |
protoporphyrin IX | mutant Q248P/S249G/K250P/G252W, pH 8.1, 30°C | Mus musculus | |
| 0.01125 | - |
protoporphyrin IX | mutant K250M/V251L/W256Y, pH 8.1, 30°C | Mus musculus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
x * 42000, wild-type, SDS-PAGE | Mus musculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| protoporphyrin + Fe2+ | Mus musculus | - |
protoheme + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | P22315 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant wild-type and mutants P255R, S249A/K250Q/V251C, K250M/V251L/W256Y, Q248P/S249G/K250P/G252W | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| protoporphyrin + Fe2+ | - |
Mus musculus | protoheme + H+ | - |
? | |
| protoporphyrin IX + Fe2+ | pH 8.1, 30°C | Mus musculus | protoheme + 2 H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 42000, wild-type, SDS-PAGE | Mus musculus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ferrochelatase | - |
Mus musculus |
| protoheme ferrolyase | - |
Mus musculus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
Fe2+ | mutant K250M/V251L/W256Y, pH 8.1, 30°C | Mus musculus | |
| 0.05 | - |
protoporphyrin IX | mutant K250M/V251L/W256Y, pH 8.1, 30°C | Mus musculus | |
| 0.068 | - |
Fe2+ | wild-type, pH 8.1, 30°C | Mus musculus | |
| 0.068 | - |
protoporphyrin IX | wild-type, pH 8.1, 30°C | Mus musculus | |
| 0.13 | - |
Fe2+ | mutant P255R, pH 8.1, 30°C | Mus musculus | |
| 0.13 | - |
protoporphyrin IX | mutant P255R, pH 8.1, 30°C | Mus musculus | |
| 0.138 | - |
Fe2+ | mutant V251L, pH 8.1, 30°C | Mus musculus | |
| 0.138 | - |
protoporphyrin IX | mutant V251L, pH 8.1, 30°C | Mus musculus | |
| 0.235 | - |
Fe2+ | mutant Q248P/S249G/K250P/G252W, pH 8.1, 30°C | Mus musculus | |
| 0.235 | - |
protoporphyrin IX | mutant Q248P/S249G/K250P/G252W, pH 8.1, 30°C | Mus musculus | |
| 0.3 | - |
Fe2+ | mutant S249A/K250Q/V251C, pH 8.1, 30°C | Mus musculus | |
| 0.3 | - |
protoporphyrin IX | mutant S249A/K250Q/V251C, pH 8.1, 30°C | Mus musculus | |
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