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Literature summary for 4.8.1.2 extracted from

  • Betke, T.; Higuchi, J.; Rommelmann, P.; Oike, K.; Nomura, T.; Kato, Y.; Asano, Y.; Groeger, H.
    biocatalytic synthesis of nitriles through dehydration of aldoximes The substrate scope of aldoxime dehydratases (2018), ChemBioChem, 19, 768-779 .
    View publication on PubMed
Search for more literature for 4.8.1.2

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
76400
-
 gel filtration Pseudomonas chlororaphis

Organism

Organism UniProt Comment Textmining
Pseudomonas chlororaphis
-
-
-
Pseudomonas chlororaphis B23
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(1E)-2-(1,3-benzodioxol-5-yl)-N-hydroxypropan-1-imine 17% conversion, 56% ee (S) Pseudomonas chlororaphis (2S)-2-(1,3-benzodioxol-5-yl)propanenitrile + H2O
-
 ?
(1E)-2-(1,3-benzodioxol-5-yl)-N-hydroxypropan-1-imine 17% conversion, 56% ee (S) Pseudomonas chlororaphis B23 (2S)-2-(1,3-benzodioxol-5-yl)propanenitrile + H2O
-
 ?
(1E)-N-hydroxy-2,6-dimethylhept-5-en-1-imine 38% conversion, 43% ee (+) Pseudomonas chlororaphis (2S)-2,6-dimethylhept-5-en-1-imine + H2O
-
 ?
(1E)-N-hydroxy-2,6-dimethylhept-5-en-1-imine 38% conversion, 43% ee (+) Pseudomonas chlororaphis B23 (2S)-2,6-dimethylhept-5-en-1-imine + H2O
-
 ?
(1E)-N-hydroxy-2-(thiophen-2-yl)propan-1-imine 18% conversion, 35% ee (-) Pseudomonas chlororaphis (2R)-2-(thiophen-2-yl)propanenitrile + H2O
-
 ?
(1E)-N-hydroxy-2-(thiophen-2-yl)propan-1-imine 18% conversion, 35% ee (-) Pseudomonas chlororaphis B23 (2R)-2-(thiophen-2-yl)propanenitrile + H2O
-
 ?
(1E)-N-hydroxy-2-phenylpropan-1-imine 26% conversion, 91% ee (S) Pseudomonas chlororaphis (2S)-2-phenylpropanenitrile + H2O
-
 ?
(1E)-N-hydroxy-2-phenylpropan-1-imine 26% conversion, 91% ee (S) Pseudomonas chlororaphis B23 (2S)-2-phenylpropanenitrile + H2O
-
 ?
(1E)-N-hydroxybutan-1-imine
-
 Pseudomonas chlororaphis butanenitrile + H2O
-
 ?
(1E)-N-hydroxybutan-1-imine
-
 Pseudomonas chlororaphis B23 butanenitrile + H2O
-
 ?
(1E)-N-hydroxyethanimine
-
 Pseudomonas chlororaphis acetonitrile + H2O
-
 ?
(Z)-1-(cyclohex-3-en-1-yl)-N-hydroxymethanimine 54% conversion, 4% ee (+) Pseudomonas chlororaphis (1S)-cyclohex-3-ene-1-carbonitrile + H2O
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 40129, calculated from amino acid sequence Pseudomonas chlororaphis

Synonyms

Synonyms Comment Organism
OxdA
-
 Pseudomonas chlororaphis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Pseudomonas chlororaphis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 the enzyme is stable at temperatures below 40°C Pseudomonas chlororaphis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Pseudomonas chlororaphis

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8 the enzyme is stable in the pH range 6.0 to 8.0 Pseudomonas chlororaphis