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Literature summary for 4.8.1.2 extracted from

  • Miao, Y.; Metzner, R.; Asano, Y.
    Kemp elimination catalyzed by naturally occurring aldoxime dehydratases (2017), ChemBioChem, 18, 451-454 .
    View publication on PubMed
Search for more literature for 4.8.1.2

Application

Application Comment Organism
synthesis enzyme is able to catalyze the non-natural Kemp elimination reaction Pseudomonas chlororaphis
synthesis enzyme is able to catalyze the non-natural Kemp elimination reaction Bacillus sp. OxB-1
synthesis enzyme is able to catalyze the non-natural Kemp elimination reaction Rhodococcus sp. N-771

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas chlororaphis
expression in Escherichia coli Bacillus sp. OxB-1
expression in Escherichia coli Rhodococcus sp. N-771

Protein Variants

Protein Variants Comment Organism
F289A about 45% of wild-type activity Bacillus sp. OxB-1
H282A about 5% of wild-type activity Bacillus sp. OxB-1
H306A about 3% of wild-type activity Bacillus sp. OxB-1
L128A about 30% of wild-type activity Bacillus sp. OxB-1
Q204A activity similar to wild-type Bacillus sp. OxB-1
T202A about 20% of wild-type activity in absence, 50% in presence of dithionite Bacillus sp. OxB-1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
 5-nitro-1,2-benzoxazole ferrous oxidation state, pH 7.0, 23°C Pseudomonas chlororaphis
0.26
-
 5-nitro-1,2-benzoxazole ferrous oxidation state, pH 7.0, 23°C Rhodococcus sp. N-771
0.55
-
 5-nitro-1,2-benzoxazole ferric oxidation state, pH 7.0, 23°C Bacillus sp. OxB-1
0.56
-
 5-nitro-1,2-benzoxazole ferric oxidation state, pH 7.0, 23°C Rhodococcus sp. N-771
0.81
-
 5-nitro-1,2-benzoxazole ferric oxidation state, pH 7.0, 23°C Pseudomonas chlororaphis
0.84
-
 5-nitro-1,2-benzoxazole ferrous oxidation state, pH 7.0, 23°C Bacillus sp. OxB-1

Organism

Organism UniProt Comment Textmining
Bacillus sp. OxB-1 A0A0A8JIJ3
-
-
Pseudomonas chlororaphis Q7WSJ4
-
-
Rhodococcus sp. N-771 Q76K71
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-nitro-1,2-benzoxazole
-
 Pseudomonas chlororaphis 2-hydroxy-5-nitrobenzonitrile
-
 ?
5-nitro-1,2-benzoxazole
-
 Bacillus sp. OxB-1 2-hydroxy-5-nitrobenzonitrile
-
 ?
5-nitro-1,2-benzoxazole
-
 Rhodococcus sp. N-771 2-hydroxy-5-nitrobenzonitrile
-
 ?

Synonyms

Synonyms Comment Organism
OxdB
-
 Bacillus sp. OxB-1

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.08
-
 5-nitro-1,2-benzoxazole ferric oxidation state, pH 7.0, 23°C Rhodococcus sp. N-771
0.14
-
 5-nitro-1,2-benzoxazole ferric oxidation state, pH 7.0, 23°C Bacillus sp. OxB-1
0.18
-
 5-nitro-1,2-benzoxazole ferric oxidation state, pH 7.0, 23°C Pseudomonas chlororaphis
9.58
-
 5-nitro-1,2-benzoxazole ferrous oxidation state, pH 7.0, 23°C Bacillus sp. OxB-1
9.97
-
 5-nitro-1,2-benzoxazole ferrous oxidation state, pH 7.0, 23°C Pseudomonas chlororaphis
17.03
-
 5-nitro-1,2-benzoxazole ferrous oxidation state, pH 7.0, 23°C Rhodococcus sp. N-771

Cofactor

Cofactor Comment Organism Structure
heme the heme iron after purification is primarily in the ferric form. For Kemp elimination, the enzymatic rate over background with ferrous enzyme is approximately 10fold higher than for ferric enzyme Pseudomonas chlororaphis
heme the heme iron after purification is primarily in the ferric form. For Kemp elimination, the enzymatic rate over background with ferrous enzyme is approximately 10fold higher than for ferric enzyme Bacillus sp. OxB-1
heme the heme iron after purification is primarily in the ferric form. For Kemp elimination, the enzymatic rate over background with ferrous enzyme is approximately 10fold higher than for ferric enzyme Rhodococcus sp. N-771

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.143
-
 5-nitro-1,2-benzoxazole ferric oxidation state, pH 7.0, 23°C Rhodococcus sp. N-771
0.216
-
 5-nitro-1,2-benzoxazole ferric oxidation state, pH 7.0, 23°C Pseudomonas chlororaphis
0.227
-
 5-nitro-1,2-benzoxazole ferric oxidation state, pH 7.0, 23°C Bacillus sp. OxB-1
11.46
-
 5-nitro-1,2-benzoxazole ferrous oxidation state, pH 7.0, 23°C Bacillus sp. OxB-1
65.57
-
 5-nitro-1,2-benzoxazole ferrous oxidation state, pH 7.0, 23°C Rhodococcus sp. N-771
66.34
-
 5-nitro-1,2-benzoxazole ferrous oxidation state, pH 7.0, 23°C Pseudomonas chlororaphis