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Literature summary for 4.8.1.2 extracted from

  • Nomura, J.; Hashimoto, H.; Ohta, T.; Hashimoto, Y.; Wada, K.; Naruta, Y.; Oinuma, K.; Kobayashi, M.
    Crystal structure of aldoxime dehydratase and its catalytic mechanism involved in carbon-nitrogen triple-bond synthesis (2013), Proc. Natl. Acad. Sci. USA, 110, 2810-2815.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.8.1.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL cells Pseudomonas chlororaphis

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 100 mM CAPSO (pH 9.4), 2.0 M ammonium sulfate, and 12% (v/v) glycerol Pseudomonas chlororaphis

Protein Variants

Protein Variants Comment Organism
E143A the mutant shows about wild type enzyme activity Pseudomonas chlororaphis
E143A/R178A the mutant shows 29.4% activity compared to the wild type enzyme Pseudomonas chlororaphis
H169A the mutant shows 123% activity compared to the wild type enzyme Pseudomonas chlororaphis
H296A the mutant shows 141% activity compared to the wild type enzyme Pseudomonas chlororaphis
H320A the mutant shows 0.137% activity compared to the wild type enzyme Pseudomonas chlororaphis
N279A the mutant shows 53.1% activity compared to the wild type enzyme Pseudomonas chlororaphis
Q221A the mutant shows about wild type enzyme activity Pseudomonas chlororaphis
R178A the mutant exhibits 19.1% of wild type activity and 1.62% of wild type heme content Pseudomonas chlororaphis
S174A the mutant shows about wild type enzyme activity Pseudomonas chlororaphis
S219A the mutant has completely lost activity despite unaffected heme content Pseudomonas chlororaphis
S219C the mutant shows 2.01% activity compared to the wild type enzyme Pseudomonas chlororaphis
S219T the mutant shows 26.1% activity compared to the wild type enzyme Pseudomonas chlororaphis
Y234A the mutant shows 170% activity compared to the wild type enzyme Pseudomonas chlororaphis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(E/Z)-n-butyraldoxime Pseudomonas chlororaphis
-
 n-butyronitrile + H2O
-
 ?
(E/Z)-n-butyraldoxime Pseudomonas chlororaphis B23
-
 n-butyronitrile + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas chlororaphis Q7WSJ4
-
-
Pseudomonas chlororaphis B23 Q7WSJ4
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas chlororaphis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(E/Z)-n-butyraldoxime
-
 Pseudomonas chlororaphis n-butyronitrile + H2O
-
 ?
(E/Z)-n-butyraldoxime
-
 Pseudomonas chlororaphis B23 n-butyronitrile + H2O
-
 ?

Synonyms

Synonyms Comment Organism
OxdA
-
 Pseudomonas chlororaphis

Cofactor

Cofactor Comment Organism Structure
heme
-
 Pseudomonas chlororaphis