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Literature summary for 4.8.1.2 extracted from

  • Konishi, K.; Ohta, T.; Oinuma, K.; Hashimoto, Y.; Kitagawa, T.; Kobayashi, M.
    Discovery of a reaction intermediate of aliphatic aldoxime dehydratase involving heme as an active center (2006), Proc. Natl. Acad. Sci. USA, 103, 564-568.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.8.1.2

Application

Application Comment Organism
industry production of nitriles Pseudomonas chlororaphis

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Pseudomonas chlororaphis

Organism

Organism UniProt Comment Textmining
Pseudomonas chlororaphis
-
-
-
Pseudomonas chlororaphis
-
 B23
-
Pseudomonas chlororaphis B23
-
-
-
Pseudomonas chlororaphis B23
-
 B23
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas chlororaphis
by ammonium sulfate fractionation and DEAE-Sephacel, TSK gel Butyl-Toyopearl 650M and Cellulofine HAp column chromatography Pseudomonas chlororaphis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
an aliphatic aldoxime
-
 Pseudomonas chlororaphis an aliphatic nitrile + H2O
-
 ?
an aliphatic aldoxime
-
 Pseudomonas chlororaphis B23 an aliphatic nitrile + H2O
-
 ?
butyraldoxime
-
 Pseudomonas chlororaphis butyronitrile + H2O addition of 200 mM butyraldoxime to ferrous OxdA yields a longlived OxdA-substrate complex, named OS-II ?
butyraldoxime
-
 Pseudomonas chlororaphis B23 butyronitrile + H2O addition of 200 mM butyraldoxime to ferrous OxdA yields a longlived OxdA-substrate complex, named OS-II ?
n-butyraldoxime reaction intermediate of the heme-containing enzyme with a highly oxidized heme that is formed concomitantly upon direct binding of the substrate, heme directly activates the organic substrate Pseudomonas chlororaphis n-butyronitrile + H2O
-
 ?
n-butyraldoxime reaction intermediate of the heme-containing enzyme with a highly oxidized heme that is formed concomitantly upon direct binding of the substrate, heme directly activates the organic substrate Pseudomonas chlororaphis B23 n-butyronitrile + H2O
-
 ?

Synonyms

Synonyms Comment Organism
aliphatic aldoxime dehydratase
-
 Pseudomonas chlororaphis
OXD
-
 Pseudomonas chlororaphis
OxdA
-
 Pseudomonas chlororaphis

Cofactor

Cofactor Comment Organism Structure
heme heme directly activates the organic substrate Pseudomonas chlororaphis