Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.6.1.19 extracted from

  • Thorn, A.; Steinfeld, R.; Ziegenbein, M.; Grapp, M.; Hsiao, H.H.; Urlaub, H.; Sheldrick, G.M.; Gaertner, J.; Kraetzner, R.
    Structure and activity of the only human RNase T2 (2012), Nucleic Acids Res., 40, 8733-8742.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.6.1.19

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of His-tagged enzyme Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
purified enzyme, hanging drop vapour diffusion method, mixing of 0.002 ml of 9.2 mg/ml protein in 20 mM acetate, pH 6.0, and 20 mM NaCl, with 0.002 ml of reservoir solution containing 0.2 M NH4NO3 and 15% PEG 3350, equilibration over 0.6 ml of reservoir solution, X-ray diffraction structure determination and analysis at 1.6 A resolution Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ complete inhibition at 50 mM Homo sapiens
Zn2+ complete inhibition at 50 mM Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no significant inhibitory effect of cobalt, nickel or magnesium at 50 mM concentration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens O00584
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein N-linked glycoprotein, enzyme activity is independent of glycosylation, deglycosylation by EndoH leaving one N-acetyl glucosamine residue at each of the N-glycosylation sites Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme by metal affinity chromatography, deglycosylation, concanavalin A affinity chromatography and gel filtration Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Homo sapiens
-
white matter
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information substrate is yeast RNA Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
RNase T2
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.6 6 assay at Homo sapiens

General Information

General Information Comment Organism
evolution RNase T2 is the only member of the Rh/T2/S family of acidic hydrolases in humans, its structure shows a typical fold for members of the T2 RNase family with seven alpha-helices and eight beta-strands constituting an alpha+beta motif, as most of the central anti-parallel beta-sheet is clearly separated in the sequence from the helical parts. The alpha + beta core fold shows high similarity to those of known T2 RNase structures from plants, while, in contrast, the external loop regions show distinct structural differences Homo sapiens
malfunction mutations in the gene of human enzyme RNase T2 are associated with white matter disease of the human brain showing bilateral temporal lobe cysts and multifocal white matter lesions which lead to psychomotor impairments,spasticity and epilepsy Homo sapiens
additional information enzyme mode of action independent of its enzymatic activity, overview. The enzyme has four disulfide bridges, connecting cysteine residues 48/55, 75/121, 184/241 and 202/213, its catalytic site comprises residues His65, His113, Glu114, Lys117 and His118 Homo sapiens