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Information on EC 4.6.1.19 - ribonuclease T2

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IUBMB Comments
A widely distributed family of related enzymes found in protozoans, plants, bacteria, animals and viruses that cleave ssRNA 3'-phosphate group with little base specificity. The enzyme catalyses a two-stage endonucleolytic cleavage. The first reaction produces 5'-hydroxy-phosphooligonucletides and 3'-phosphooligonucleotides ending with a 2',3'-cyclic phosphodiester, which are released from the enzyme. The enzyme then hydrolyses the cyclic products in a second reaction that takes place only when all the susceptible 3',5'-phosphodiester bonds have been cyclised. The second reaction is a reversal of the first reaction using the hydroxyl group of water instead of the 5'-hydroxyl group of ribose. The overall process is that of a phosphorus-oxygen lyase followed by hydrolysis to form the 3'-nucleotides.
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Word Map
The enzyme appears in viruses and cellular organisms
Reaction Schemes
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an [RNA fragment]-3'-nucleoside-3'-phosphate
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a 5'-hydroxy-ribonucleotide-3'-[RNA fragment]
Synonyms
acid ribonuclease, acid RNase, ACTIBIND, base non-specific ribonuclease, base nonspecific RNase, base-non-specific ribonuclease, base-nonspecific RNase, base-nonspecific RNase Rh, BSP1, colicin E5, more
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
an [RNA fragment]-3'-nucleoside-2',3'-cyclophosphate + H2O = an [RNA fragment]-3'-nucleoside-3'-phosphate
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(1b)
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RNA + H2O = an [RNA fragment]-3'-nucleoside-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA fragment]
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RNA = an [RNA fragment]-3'-nucleoside-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA fragment]
show the reaction diagram
(1a)
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