Literature summary for 4.6.1.19 extracted from

Tanaka, N.; Arai, J.; Inokuchi, N.; Koyama, T.; Ohgi, K.; Irie, M.; Nakamura, K.T.
Crystal structure of a plant ribonuclease, RNase LE (2000), J. Mol. Biol., 298, 859-873.

Crystallization (Commentary)

Crystallization (Comment)	Organism
20 mg/ml purified recombinant wild-type enzyme, hanging drop vapour diffusion method, equal volumes of protein solution, 20 mM citrate, pH 5.0, and reservoir solution, 0.1 M citrate, pH 5.0, 36% w/v PEG 1540, X-ray diffraction structure determination and analysis at 1.65-2.2 A resolution of wild-type enzyme crystals and heavy atom derivatives	Solanum lycopersicum

Protein Variants	Comment	Organism
H109F	site-directed mutagenesis, 35% activity compared to the wild-type enzyme	Solanum lycopersicum
H134F	site-directed mutagenesis, 49% activity compared to the wild-type enzyme	Solanum lycopersicum
H39F	site-directed mutagenesis, conserved active site residue, 0.18% activity compared to the wild-type enzyme	Solanum lycopersicum
H92F	site-directed mutagenesis, conserved active site residue, 0.21% activity compared to the wild-type enzyme	Solanum lycopersicum
H97F	site-directed mutagenesis, conserved active site residue, 0.12% activity compared to the wild-type enzyme	Solanum lycopersicum

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
24000	-	x * 24000, about	Solanum lycopersicum

Organism	UniProt	Comment	Textmining
Solanum lycopersicum	P80022	-	-

Reaction	Comment	Organism	Reaction ID
RNA + H2O = an [RNA fragment]-3'-nucleoside-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA fragment]	catalytic hydrolysis mechanism, active site structure, the conserved active site residues His39, His92, and His 97 are essential for activity, Glu93 and Lys96 are important	Solanum lycopersicum	a

Source Tissue	Comment	Organism	Textmining
cell culture	-	Solanum lycopersicum	-

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	broad substrate specificity	Solanum lycopersicum	?	-	?
RNA + H2O	-	Solanum lycopersicum	nucleoside 3'-phosphates and 3'-phosphooligonucleotides	via oligonucleotides or mononucleotides with 2',3'-cyclic phosphate at the 3'-side	?

Subunits	Comment	Organism
?	x * 24000, about	Solanum lycopersicum
More	disulfide bridge pattern	Solanum lycopersicum

Synonyms	Comment	Organism
acid ribonuclease	-	Solanum lycopersicum
acid RNase	-	Solanum lycopersicum
base-non-specific ribonuclease	-	Solanum lycopersicum
Escherichia coli ribonuclease I' ribonuclease PP2'	-	Solanum lycopersicum
Escherichia coli ribonuclease II	-	Solanum lycopersicum
More	enzyme belongs to the ribonuclease T2 family, structure comparison and amino acid sequence alignment	Solanum lycopersicum
non-base specific ribonuclease	-	Solanum lycopersicum
nonbase-specific RNase	-	Solanum lycopersicum
nonspecific RNase	-	Solanum lycopersicum
ribonnuclease (non-base-specific)	-	Solanum lycopersicum
ribonuclease (non-base-specific)	-	Solanum lycopersicum
ribonuclease 3'-oligonucleotide hydrolase	-	Solanum lycopersicum
ribonuclease II	-	Solanum lycopersicum
ribonuclease LE	-	Solanum lycopersicum
ribonuclease M	-	Solanum lycopersicum
ribonuclease N2	-	Solanum lycopersicum
ribonuclease nucleotido-2'-transferase (cyclizing)	-	Solanum lycopersicum
ribonuclease PP3	-	Solanum lycopersicum
ribonuclease U4	-	Solanum lycopersicum
RNase (non-base specific)	-	Solanum lycopersicum
RNase CL	-	Solanum lycopersicum
RNase II	-	Solanum lycopersicum
RNase LE	-	Solanum lycopersicum
RNase M	-	Solanum lycopersicum
RNase Ms	-	Solanum lycopersicum
RNase T2	-	Solanum lycopersicum

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Solanum lycopersicum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5.5	-	-	Solanum lycopersicum