Literature summary for 4.6.1.18 extracted from

Kurpiewska, K.; Font, J.; Ribo, M.; Vilanova, M.; Lewi?ski, K.
X-ray crystallographic studies of RNase A variants engineered at the most destabilizing positions of the main hydrophobic core: further insight into protein stability (2009), Proteins, 77, 658-669.

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli strain BL21	Bos taurus

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method, ammonium sulfate, sodium chloride, sodium acetate, pH 6.0, crystal structure of bovine pancreatic ribonuclease A (wild-type), resolution 1.60 A, crystal structure of bovine pancreatic ribonuclease A variant V47A, resolution 1.60 A, crystal structure of bovine pancreatic ribonuclease A variant V54A, resolution 1.60 A, crystal structure of bovine pancreatic ribonuclease A variant V57A, resolution 1.60 A, crystal structure of bovine pancreatic ribonuclease A variant I81A, resolution 2.0 A, crystal structure of bovine pancreatic ribonuclease A variant I106A, resolution 1.40 A, crystal structure of bovine pancreatic ribonuclease A variant V108A, resolution 1.60 A, space group P3221	Bos taurus

Crystallization (Comment)

Organism

hanging drop vapor diffusion method, ammonium sulfate, sodium chloride, sodium acetate, pH 6.0, crystal structure of bovine pancreatic ribonuclease A (wild-type), resolution 1.60 A, crystal structure of bovine pancreatic ribonuclease A variant V47A, resolution 1.60 A, crystal structure of bovine pancreatic ribonuclease A variant V54A, resolution 1.60 A, crystal structure of bovine pancreatic ribonuclease A variant V57A, resolution 1.60 A, crystal structure of bovine pancreatic ribonuclease A variant I81A, resolution 2.0 A, crystal structure of bovine pancreatic ribonuclease A variant I106A, resolution 1.40 A, crystal structure of bovine pancreatic ribonuclease A variant V108A, resolution 1.60 A, space group P3221

Bos taurus

Protein Variants

Protein Variants	Comment	Organism
I106A	site-directed mutagenesis	Bos taurus
I81A	site-directed mutagenesis	Bos taurus
additional information	the structure of bovine pancreatic ribonuclease A variants V47A, V54A, V57A, I81A, I106A, and V108A is solved at 1.4-2.0 A resolution and compared with the structure of wild-type protein. The introduced mutations have only minor influence on the global structure of ribonuclease A. The structural changes have individual character that depends on the localization of mutated residue, however, they seem to expand from mutation site to the rest of the structure. Analysis of the difference distance matrices reveal that the ribonuclease A molecule is organized into five relatively rigid subdomains with individual response to mutation	Bos taurus
V108A	site-directed mutagenesis	Bos taurus
V47A	site-directed mutagenesis	Bos taurus
V54A	site-directed mutagenesis	Bos taurus
V57A	site-directed mutagenesis	Bos taurus

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	P61823	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Bos taurus

Source Tissue

Source Tissue	Comment	Organism	Textmining

Synonyms

Synonyms	Comment	Organism
pancreatic ribonuclease A	-	Bos taurus