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Literature summary for 4.6.1.18 extracted from

  • Moussaoui, M.; Cuchillo, C.M.; Nogues, M.V.
    A phosphate-binding subsite in bovine pancreatic ribonuclease A can be converted into a very efficient catalytic site (2007), Protein Sci., 16, 99-109.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.6.1.18

Protein Variants

Protein Variants Comment Organism
H12K/H119Q 0.007% of wild-type activity Bos taurus
K7H/R10H 17% of wild-type activity, introduction of a putative new catalytic site resulting in increase in exonucleolytic activity Bos taurus
K7H/R10H/H12K/H119Q 9% of wild-type activity due to suppression of native active site, increase in exonucleolytic activity Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0023
-
 cyclic 2',3'-cytidine monophosphate wild-type, pH 5.5, 25°C Bos taurus
0.0026
-
 cyclic 2',3'-cytidine monophosphate mutant K7H/R10H, pH 5.5, 25°C Bos taurus
0.015
-
 pentacytidylic acid mutant K7H/R10H/H12K/H119Q, pH 7.0 Bos taurus
0.028
-
 pentacytidylic acid mutant K7H/R10H, pH 7.0 Bos taurus
0.038
-
 pentacytidylic acid wild-type, pH7.0 Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus P61823
-
-

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cyclic 2',3'-cytidine monophosphate + H2O
-
 Bos taurus 3'-CMP
-
 ?
pentacytidylic acid + H2O
-
 Bos taurus ?
-
 ?
poly(C) + H2O
-
 Bos taurus 3'-CMP + 3'-phospho-oligo(C)
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5
-
 cyclic 2',3'-cytidine monophosphate mutant K7H/R10H, pH 5.5, 25°C Bos taurus
12
-
 cyclic 2',3'-cytidine monophosphate wild-type, pH 5.5, 25°C Bos taurus
27
-
 pentacytidylic acid mutant K7H/R10H/H12K/H119Q, pH 7.0 Bos taurus
54
-
 pentacytidylic acid mutant K7H/R10H, pH 7.0 Bos taurus
323
-
 pentacytidylic acid wild-type, pH 7.0 Bos taurus