Literature summary for 4.6.1.18 extracted from

Leich, F.; Koeditz, J.; Ulbrich-Hofman, R.; Arnold, U.
Tandemization endows bovine pancreatic ribonuclease with cytotoxic activity (2006), J. Mol. Biol., 358, 1305-1313.

Search for more literature for 4.6.1.18

Application

Application	Comment	Organism
medicine	use of gene duplication to generate tandem enzymes covalently bound by peptide linker. Tandemization has minor effects on the activity and stability in comparison to monomeric RNase A. Relative activity decreases by 10-50%, and melting temperature decreases by less than 2.5 K. Tandemization results in remarkable cytotoxicity, decreasing the IC50 values with K-562 cells to 0.070-0.013 mM	Bos taurus

Protein Variants

Protein Variants	Comment	Organism
additional information	use of gene duplication to generate tandem enzymes covalently bound by peptide linker. Tandemization has minor effects on the activity and stability in comparison to monomeric RNase A. Relative activity decreases by 10-50%, and melting temperature decreases by less than 2.5 K. Tandemization results in remarkable cytotoxicity, decreasing the IC50 values with K-562 cells to 0.070-0.013 mM	Bos taurus

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	P61823	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
pancreas	-	Bos taurus	-

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Release 2023.1 (January 2023)