Application | Comment | Organism |
---|---|---|
medicine | cross-linking of enzyme or its covalently linked dimer to polyspermine using dimethyl suberimidate. The in vitro and in vivo cytotoxic activity of treated monomeric enzyme is not higher than that of free polyspermine, but dimeric suberimidate-treated enzyme proves to be a more efficinet antitumor agent both in vitro and in vivo | Bos taurus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | cross-linking of enzyme or its covalently linked dimer to polyspermine using dimethyl suberimidate. The in vitro and in vivo cytotoxic activity of treated monomeric enzyme is not higher than that of free polyspermine, but dimeric suberimidate-treated enzyme proves to be a more efficient antitumor agent both in vitro and in vivo | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
Type XII-A, commercial preparation | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pancreas | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.14 | - |
substrate poly(A)poly(U), dimethyl suberimidate-treated, monomeric RNase A, pH 7.0, 25°C | Bos taurus |
1.55 | - |
substrate poly(A)poly(U), native, monomeric RNase A, pH 7.0, 25°C | Bos taurus |
2.22 | - |
substrate poly(A)poly(U), cross-linked RNase A dimer, pH 7.0, 25°C | Bos taurus |
12.15 | - |
substrate poly(A)poly(U), domain-swapped RNase A C-dimer pH 7.0, 25°C | Bos taurus |
12.67 | - |
substrate yeast RNA, cross-linked RNase A dimer, pH 7.0, 25°C | Bos taurus |
13.33 | - |
substrate yeast RNA, cross-linked RNase A dimer, treated with dimethyl suberimidate, pH 7.0, 25°C | Bos taurus |
25.21 | - |
substrate poly(A)poly(U), cross-linked RNase A dimer, treated with dimethyl suberimidate, pH 7.0, 25°C | Bos taurus |
49.33 | - |
substrate yeast RNA, dimethyl suberimidate-treated, monomeric RNase A, pH 7.0, 25°C | Bos taurus |
53.46 | - |
substrate yeast RNA, domain-swapped RNase A C-dimer pH 7.0, 25°C | Bos taurus |
84.55 | - |
substrate yeast RNA, native, monomeric RNase A, pH 7.0, 25°C | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
poly(A)poly(U) + H2O | - |
Bos taurus | ? | - |
? | |
RNA + H2O | substrate yeast RNA | Bos taurus | 3'-phosphomononucleotides + 3'-phosphooligonucleotides | - |
? |