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Literature summary for 4.6.1.18 extracted from

  • Fujii, T.; Ueno, H.; Hayashi, R.
    Significance of the four carboxyl terminal amino acid residues of bovine pancreatic ribonuclease A for structural folding (2002), J. Biochem., 131, 193-200.
    View publication on PubMed
Search for more literature for 4.6.1.18

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bos taurus

Protein Variants

Protein Variants Comment Organism
D121A with nearly the same Km value as the wild type enzyme but with lower hydrolytic activity Bos taurus
D121A/S123A/V124A mutant in which all of the C-terminal four amino acid residues are replaced by alanine residues, with a final lower hydrolytic activity Bos taurus
D121E with nearly the same Km value as the wild type enzyme but with with lower hydrolytic activity Bos taurus
D121K with nearly the same Km value as the wild type enzyme but with with lower hydrolytic activity Bos taurus
DELTA121-124 C-terminal deletion mutant, 14ºC less stable to thermal denaturation than the wild type enzyme Bos taurus
DELTA122-124 C-terminal deletion mutant, 3-6ºC less stable to thermal denaturation than the wild type enzyme Bos taurus
DELTA123-124 C-terminal deletion mutant, 3-6ºC less stable to thermal denaturation than the wild type enzyme Bos taurus
DELTA124 C-terminal deletion mutant, 3-6ºC less stable to thermal denaturation than the wild type enzyme Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.31
-
 cytidine-2',3'-cyclic monophosphate D121K mutant, pH 5.5, 25°C Bos taurus
0.46
-
 cytidine-2',3'-cyclic monophosphate wild type enzyme, pH 5.5, 25°C Bos taurus
0.52
-
 cytidine-2',3'-cyclic monophosphate D121A mutant, pH 5.5, 25°C Bos taurus
0.58
-
 cytidine-2',3'-cyclic monophosphate Ala(121-124) mutant, pH 5.5, 25°C Bos taurus
0.59
-
 cytidine-2',3'-cyclic monophosphate D121E mutant, pH 5.5, 25°C Bos taurus
0.64
-
 cytidine-2',3'-cyclic monophosphate des-(123-124) mutant, pH 5.5, 25°C Bos taurus
0.66
-
 cytidine-2',3'-cyclic monophosphate des-124 mutant, pH 5.5, 25°C Bos taurus
2
-
 cytidine-2',3'-cyclic monophosphate des-(121-124) mutant, pH 5.5, 25°C Bos taurus
3.1
-
 cytidine-2',3'-cyclic monophosphate des-(122-124) mutant, pH 5.5, 25°C Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus P61823 bovine
-

Purification (Commentary)

Purification (Comment) Organism
cation exchange chromatography Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
pancreas
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cytidine-2',3'-cyclic monophosphate + H2O
-
 Bos taurus 3'-CMP
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 des-124, des-(123-124), des-(122-124) mutants are 3-6°C less stable to thermal denaturation with respect to the wild type enzyme. des-(121-124) mutant is 14°C less stable to thermal denaturation with respect to the wild type enzyme Bos taurus