Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.5.1.5 extracted from

  • Kumagai, H.; Suzuki, H.; Shigematsu, H.; Tochikura, T.
    S-Carboxymethylcysteine synthase from Escherichia coli (1989), Agric. Biol. Chem., 53, 2481-2487.
No PubMed abstract available
Search for more literature for 4.5.1.5

Crystallization (Commentary)

Crystallization (Comment) Organism
fine yellow needles Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibited by the substrate 3-chloro-L-alanine Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
15.4
-
 thioglycolate pH 7.8, 37°C Escherichia coli
40
-
 3-chloro-L-alanine pH 7.8, 37°C Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
 2 * 37000, SDS-PAGE Escherichia coli
42000
-
 2 * 42000, HPLC gel filtration in presence of SDS Escherichia coli
84000
-
 HPLC gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Escherichia coli constitutive enzyme ?
-
 ?
additional information Escherichia coli W 3110 constitutive enzyme ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli W 3110
-
-
-

Purification (Commentary)

Purification (Comment) Organism
190fold Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Escherichia coli

Storage Stability

Storage Stability Organism
long-term storage of enzyme as ammonium sulfate precipitate decreases activity, reactivation by dialysis against 0.1 M potassium phosphate buffer, pH 7.8, containing 10 mM 2-mercaptoethanol, 0.02 mM pyridoxal phosphate, 4 mM EDTA Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-chloro-L-alanine + 1-thioglycerol 93% of activity compared to thioglycolate Escherichia coli S-2,3-dihydroxypropyl-L-cysteine + Cl-
-
 ?
3-chloro-L-alanine + 2-mercaptoethanol 242% of activity compared to thioglycolate Escherichia coli S-2-hydroxyethyl-L-cysteine + Cl-
-
 ?
3-chloro-L-alanine + benzylmercaptan 35% of activity compared to thioglycolate Escherichia coli S-benzyl-L-cysteine + Cl-
-
 ?
3-chloro-L-alanine + ethylmercaptan 55% of activity compared to thioglycolate Escherichia coli S-ethyl-L-cysteine + Cl-
-
 ?
3-chloro-L-alanine + ethylmercaptan 55% of activity compared to thioglycolate Escherichia coli W 3110 S-ethyl-L-cysteine + Cl-
-
 ?
3-chloro-L-alanine + methylmercaptan 65% of activity compared to thioglycolate Escherichia coli S-methyl-L-cysteine + Cl-
-
 ?
3-chloro-L-alanine + methylmercaptan 65% of activity compared to thioglycolate Escherichia coli W 3110 S-methyl-L-cysteine + Cl-
-
 ?
3-chloro-L-alanine + n-propylmercaptan 4% of activity compared to thioglycolate Escherichia coli S-propyl-L-cysteine + Cl-
-
 ?
3-chloro-L-alanine + phenylmercaptan 207% of activity compared to thioglycolate Escherichia coli S-phenyl-L-cysteine + Cl-
-
 ?
3-chloro-L-alanine + thioglycolate beta-replacement reaction Escherichia coli S-carboxymethyl-L-cysteine + Cl-
-
 ?
3-chloro-L-alanine + thioglycolate beta-replacement reaction Escherichia coli W 3110 S-carboxymethyl-L-cysteine + Cl-
-
 ?
additional information not: L-cysteine, L-serine, L-threonine, L-cystine, 3-chloro-D-alanine, DL-homocysteine, L-methionine, L-homoserine, sodium hydrosulfide, isopropylmercaptan, 2-mercaptopropionic acid, 3-mercaptopropionic acid, enzyme catalyzes the alpha,beta-elimination reaction of 3-chloro-L-alanine, L-serine and L-cystathionine at a very low rate Escherichia coli ?
-
 ?
additional information constitutive enzyme Escherichia coli ?
-
 ?
additional information not: L-cysteine, L-serine, L-threonine, L-cystine, 3-chloro-D-alanine, DL-homocysteine, L-methionine, L-homoserine, sodium hydrosulfide, isopropylmercaptan, 2-mercaptopropionic acid, 3-mercaptopropionic acid, enzyme catalyzes the alpha,beta-elimination reaction of 3-chloro-L-alanine, L-serine and L-cystathionine at a very low rate Escherichia coli W 3110 ?
-
 ?
additional information constitutive enzyme Escherichia coli W 3110 ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 37000, SDS-PAGE Escherichia coli
homodimer 2 * 42000, HPLC gel filtration in presence of SDS Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli
50
-
 S-carboxymethyl-L-cysteine synthesis Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 10 min, stable up to, S-carboxymethyl-L-cysteine synthesis Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
 assay at Escherichia coli
9 10.5 broad, S-carboxymethyl-L-cysteine synthesis Escherichia coli

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 7.5 stable pH-range, S-carboxymethyl-L-cysteine synthesis Escherichia coli

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate as cofactor, a pyridoxal phosphate protein Escherichia coli