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Information on EC 4.5.1.5 - S-carboxymethylcysteine synthase for references in articles please use BRENDA:EC4.5.1.5
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EC Tree
The expected taxonomic range for this enzyme is: Escherichia coli
Synonyms
S-carboxymethyl-L-cysteine synthase, SC-Cys synthase, synthase, S-carboxymethylcysteine,
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S-carboxymethyl-L-cysteine synthase
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synthase, S-carboxymethylcysteine
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3-chloro-L-alanine + thioglycolate = S-carboxymethyl-L-cysteine + chloride
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carbon-halide lyase reaction
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3-chloro-L-alanine chloride-lyase (adding thioglycolate; S-carboxymethyl-L-cysteine-forming)
A pyridoxal-phosphate protein.
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3-chloro-L-alanine + 1-thioglycerol
S-2,3-dihydroxypropyl-L-cysteine + Cl-
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93% of activity compared to thioglycolate
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3-chloro-L-alanine + 2-mercaptoethanol
S-2-hydroxyethyl-L-cysteine + Cl-
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242% of activity compared to thioglycolate
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3-chloro-L-alanine + benzylmercaptan
S-benzyl-L-cysteine + Cl-
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35% of activity compared to thioglycolate
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3-chloro-L-alanine + ethylmercaptan
S-ethyl-L-cysteine + Cl-
3-chloro-L-alanine + methylmercaptan
S-methyl-L-cysteine + Cl-
3-chloro-L-alanine + n-propylmercaptan
S-propyl-L-cysteine + Cl-
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4% of activity compared to thioglycolate
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3-chloro-L-alanine + phenylmercaptan
S-phenyl-L-cysteine + Cl-
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207% of activity compared to thioglycolate
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3-chloro-L-alanine + thioglycolate
S-carboxymethyl-L-cysteine + Cl-
additional information
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3-chloro-L-alanine + ethylmercaptan
S-ethyl-L-cysteine + Cl-
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55% of activity compared to thioglycolate
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3-chloro-L-alanine + ethylmercaptan
S-ethyl-L-cysteine + Cl-
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55% of activity compared to thioglycolate
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3-chloro-L-alanine + methylmercaptan
S-methyl-L-cysteine + Cl-
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65% of activity compared to thioglycolate
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3-chloro-L-alanine + methylmercaptan
S-methyl-L-cysteine + Cl-
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65% of activity compared to thioglycolate
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3-chloro-L-alanine + thioglycolate
S-carboxymethyl-L-cysteine + Cl-
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beta-replacement reaction
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3-chloro-L-alanine + thioglycolate
S-carboxymethyl-L-cysteine + Cl-
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beta-replacement reaction
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additional information
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not: L-cysteine, L-serine, L-threonine, L-cystine, 3-chloro-D-alanine, DL-homocysteine, L-methionine, L-homoserine, sodium hydrosulfide, isopropylmercaptan, 2-mercaptopropionic acid, 3-mercaptopropionic acid, enzyme catalyzes the alpha,beta-elimination reaction of 3-chloro-L-alanine, L-serine and L-cystathionine at a very low rate
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additional information
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constitutive enzyme
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not: L-cysteine, L-serine, L-threonine, L-cystine, 3-chloro-D-alanine, DL-homocysteine, L-methionine, L-homoserine, sodium hydrosulfide, isopropylmercaptan, 2-mercaptopropionic acid, 3-mercaptopropionic acid, enzyme catalyzes the alpha,beta-elimination reaction of 3-chloro-L-alanine, L-serine and L-cystathionine at a very low rate
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additional information
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constitutive enzyme
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additional information
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additional information
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constitutive enzyme
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additional information
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constitutive enzyme
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pyridoxal 5'-phosphate
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as cofactor, a pyridoxal phosphate protein
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additional information
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not inhibited by the substrate 3-chloro-L-alanine
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3-chloro-L-alanine
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pH 7.8, 37°C
15.4
thioglycolate
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pH 7.8, 37°C
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additional information
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9 - 10.5
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broad, S-carboxymethyl-L-cysteine synthesis
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50
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S-carboxymethyl-L-cysteine synthesis
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brenda
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brenda
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CYSK_ECOLI
Escherichia coli (strain K12)
323
0
34490
Swiss-Prot
A0A1J5TK03_9ZZZZ
325
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34425
TrEMBL
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37000
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2 * 37000, SDS-PAGE
42000
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2 * 42000, HPLC gel filtration in presence of SDS
84000
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HPLC gel filtration
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homodimer
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2 * 37000, SDS-PAGE; 2 * 42000, HPLC gel filtration in presence of SDS
homodimer
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2 * 37000, SDS-PAGE; 2 * 42000, HPLC gel filtration in presence of SDS
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6.5 - 7.5
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stable pH-range, S-carboxymethyl-L-cysteine synthesis
648766
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60
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10 min, stable up to, S-carboxymethyl-L-cysteine synthesis
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long-term storage of enzyme as ammonium sulfate precipitate decreases activity, reactivation by dialysis against 0.1 M potassium phosphate buffer, pH 7.8, containing 10 mM 2-mercaptoethanol, 0.02 mM pyridoxal phosphate, 4 mM EDTA
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Kumagai, H.; Suzuki, H.; Shigematsu, H.; Tochikura, T.
S-Carboxymethylcysteine synthase from Escherichia coli
Agric. Biol. Chem.
53
2481-2487
1989
Escherichia coli, Escherichia coli W 3110
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brenda
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4.5.1.5 S-carboxymethylcysteine synthase
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