Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Citrobacter freundii |
Protein Variants | Comment | Organism |
---|---|---|
C115H | mutant cleaves S-alk(en)yl-L-cysteine sulfoxides more effectively than the wild type | Citrobacter freundii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.47 | - |
(+-)-ethiin | mutant C115H, pH pH 8.0, 30°C | Citrobacter freundii | |
0.54 | - |
(+)-methiin | mutant C115H, pH pH 8.0, 30°C | Citrobacter freundii | |
0.57 | - |
(+-)-methiin | mutant C115H, pH pH 8.0, 30°C | Citrobacter freundii | |
1.1 | - |
(+-)-propiin | mutant C115H, pH pH 8.0, 30°C | Citrobacter freundii | |
1.2 | - |
(+)-alliin | mutant C115H, pH pH 8.0, 30°C | Citrobacter freundii | |
1.26 | - |
(+-)-alliin | mutant C115H, pH pH 8.0, 30°C | Citrobacter freundii | |
1.4 | - |
(+)-propiin | mutant C115H, pH pH 8.0, 30°C | Citrobacter freundii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Citrobacter freundii | Q84AR1 | cf. EC 4.4.1.11 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-alliin + H2O | - |
Citrobacter freundii | allicin + pyruvate + NH3 | - |
? | |
(+)-methiin + H2O | - |
Citrobacter freundii | methanesulfenic acid + pyruvate + NH3 | - |
? | |
(+)-propiin + H2O | - |
Citrobacter freundii | propane-1-sulfenic acid + pyruvate + NH3 | - |
? | |
(+-)-alliin + H2O | - |
Citrobacter freundii | allicin + pyruvate + NH3 | - |
? | |
(+-)-ethiin + H2O | - |
Citrobacter freundii | ethanesulfenic acid + pyruvate + NH3 | - |
? | |
(+-)-methiin + H2O | - |
Citrobacter freundii | methanesulfenic acid + pyruvate + NH3 | - |
? | |
(+-)-propiin + H2O | - |
Citrobacter freundii | propane-1-sulfenic acid + pyruvate + NH3 | - |
? | |
additional information | enzyme additionally catalyzes the beta-elimination reaction of (+-)-S-alk(en)yl-L-cysteine sulfoxides to yield thiosulfinate, reaction of EC 4.4.1.4. It can decompose both diastereomers equally | Citrobacter freundii | ? | - |
? | |
S-butyl-L-cysteine + H2O | - |
Citrobacter freundii | butanethiol + NH3 + 2-oxopropanoate | - |
? | |
S-ethyl-L-cysteine + H2O | - |
Citrobacter freundii | ethanethiol + NH3 + 2-oxopropanoate | - |
? | |
S-methyl-L-cysteine + H2O | - |
Citrobacter freundii | methanethiol + NH3 + 2-oxopropanoate | - |
? | |
S-propyl-L-cysteine + H2O | - |
Citrobacter freundii | propanethiol + NH3 + 2-oxopropanoate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.14 | - |
(+-)-methiin | mutant C115H, pH pH 8.0, 30°C | Citrobacter freundii | |
5.2 | - |
(+)-methiin | mutant C115H, pH pH 8.0, 30°C | Citrobacter freundii | |
5.59 | - |
(+-)-ethiin | mutant C115H, pH pH 8.0, 30°C | Citrobacter freundii | |
12.4 | - |
(+-)-alliin | mutant C115H, pH pH 8.0, 30°C | Citrobacter freundii | |
13.7 | - |
(+)-alliin | mutant C115H, pH pH 8.0, 30°C | Citrobacter freundii | |
20.2 | - |
(+-)-propiin | mutant C115H, pH pH 8.0, 30°C | Citrobacter freundii | |
21.8 | - |
(+)-propiin | mutant C115H, pH pH 8.0, 30°C | Citrobacter freundii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9 | - |
(+-)-methiin | mutant C115H, pH pH 8.0, 30°C | Citrobacter freundii | |
9.6 | - |
(+)-methiin | mutant C115H, pH pH 8.0, 30°C | Citrobacter freundii | |
9.8 | - |
(+-)-alliin | mutant C115H, pH pH 8.0, 30°C | Citrobacter freundii | |
11 | - |
(+)-alliin | mutant C115H, pH pH 8.0, 30°C | Citrobacter freundii | |
12 | - |
(+-)-ethiin | mutant C115H, pH pH 8.0, 30°C | Citrobacter freundii | |
16 | - |
(+)-propiin | mutant C115H, pH pH 8.0, 30°C | Citrobacter freundii | |
18 | - |
(+-)-propiin | mutant C115H, pH pH 8.0, 30°C | Citrobacter freundii |