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show all sequences of 4.3.1.31

Isolation and characterization of L-tryptophan ammonia lyase from Rubrivivax benzoatilyticus strain JA2

Kumavath, R.N.; Ramana ChV.; Sasikala, Ch.; Barh, D.; Kumar, A.P.; Azevedo, V.; Curr. Protein Pept. Sci. 16, 775-781 (2015)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00004
-
L-tryptophan
pH and temperature not specified in the publication
Rubrivivax benzoatilyticus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-tryptophan
Rubrivivax benzoatilyticus
-
3-indoleacrylate + ammonia
-
-
?
L-tryptophan
Rubrivivax benzoatilyticus JA2
-
3-indoleacrylate + ammonia
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rubrivivax benzoatilyticus
-
-
-
Rubrivivax benzoatilyticus JA2
-
-
-
Purification (Commentary)
Commentary
Organism
-
Rubrivivax benzoatilyticus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
glycine
about 30% of the activity with L-tryptophan
735994
Rubrivivax benzoatilyticus
?
-
-
-
?
L-alanine
about 30% of the activity with L-tryptophan
735994
Rubrivivax benzoatilyticus
?
-
-
-
?
L-alanine
about 30% of the activity with L-tryptophan
735994
Rubrivivax benzoatilyticus JA2
?
-
-
-
?
L-glutamate
about 60% of the activity with L-tryptophan
735994
Rubrivivax benzoatilyticus
?
-
-
-
?
L-phenylalanine
about 60% of the activity with L-tryptophan
735994
Rubrivivax benzoatilyticus
(E)-cinnamate + NH3
-
-
-
?
L-phenylalanine
about 60% of the activity with L-tryptophan
735994
Rubrivivax benzoatilyticus JA2
(E)-cinnamate + NH3
-
-
-
?
L-tryptophan
-
735994
Rubrivivax benzoatilyticus
3-indoleacrylate + ammonia
-
-
-
?
L-tryptophan
-
735994
Rubrivivax benzoatilyticus
3-indoleacrylate + ammonia
3-indoleacrylate i.e. (2E)-3-(1H-indol-3-yl)prop-2-enoate
-
-
?
L-tryptophan
-
735994
Rubrivivax benzoatilyticus JA2
3-indoleacrylate + ammonia
-
-
-
?
L-tryptophan
-
735994
Rubrivivax benzoatilyticus JA2
3-indoleacrylate + ammonia
3-indoleacrylate i.e. (2E)-3-(1H-indol-3-yl)prop-2-enoate
-
-
?
L-tyrosine
about 30% of the activity with L-tryptophan
735994
Rubrivivax benzoatilyticus
trans-p-hydroxycinnamate + NH3
-
-
-
?
L-tyrosine
about 30% of the activity with L-tryptophan
735994
Rubrivivax benzoatilyticus JA2
trans-p-hydroxycinnamate + NH3
-
-
-
?
Subunits
Subunits
Commentary
Organism
heterotetramer
-
Rubrivivax benzoatilyticus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
35
-
-
Rubrivivax benzoatilyticus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Rubrivivax benzoatilyticus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00004
-
L-tryptophan
pH and temperature not specified in the publication
Rubrivivax benzoatilyticus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-tryptophan
Rubrivivax benzoatilyticus
-
3-indoleacrylate + ammonia
-
-
?
L-tryptophan
Rubrivivax benzoatilyticus JA2
-
3-indoleacrylate + ammonia
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Rubrivivax benzoatilyticus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
glycine
about 30% of the activity with L-tryptophan
735994
Rubrivivax benzoatilyticus
?
-
-
-
?
L-alanine
about 30% of the activity with L-tryptophan
735994
Rubrivivax benzoatilyticus
?
-
-
-
?
L-alanine
about 30% of the activity with L-tryptophan
735994
Rubrivivax benzoatilyticus JA2
?
-
-
-
?
L-glutamate
about 60% of the activity with L-tryptophan
735994
Rubrivivax benzoatilyticus
?
-
-
-
?
L-phenylalanine
about 60% of the activity with L-tryptophan
735994
Rubrivivax benzoatilyticus
(E)-cinnamate + NH3
-
-
-
?
L-phenylalanine
about 60% of the activity with L-tryptophan
735994
Rubrivivax benzoatilyticus JA2
(E)-cinnamate + NH3
-
-
-
?
L-tryptophan
-
735994
Rubrivivax benzoatilyticus
3-indoleacrylate + ammonia
-
-
-
?
L-tryptophan
-
735994
Rubrivivax benzoatilyticus
3-indoleacrylate + ammonia
3-indoleacrylate i.e. (2E)-3-(1H-indol-3-yl)prop-2-enoate
-
-
?
L-tryptophan
-
735994
Rubrivivax benzoatilyticus JA2
3-indoleacrylate + ammonia
-
-
-
?
L-tryptophan
-
735994
Rubrivivax benzoatilyticus JA2
3-indoleacrylate + ammonia
3-indoleacrylate i.e. (2E)-3-(1H-indol-3-yl)prop-2-enoate
-
-
?
L-tyrosine
about 30% of the activity with L-tryptophan
735994
Rubrivivax benzoatilyticus
trans-p-hydroxycinnamate + NH3
-
-
-
?
L-tyrosine
about 30% of the activity with L-tryptophan
735994
Rubrivivax benzoatilyticus JA2
trans-p-hydroxycinnamate + NH3
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
heterotetramer
-
Rubrivivax benzoatilyticus
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
35
-
-
Rubrivivax benzoatilyticus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Rubrivivax benzoatilyticus
Other publictions for EC 4.3.1.31
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735994
Kumavath
Isolation and characterization ...
Rubrivivax benzoatilyticus, Rubrivivax benzoatilyticus JA2
Curr. Protein Pept. Sci.
16
775-781
2015
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