Cloned (Comment) | Organism |
---|---|
overexpressed in Escherichia coli | Carboxydothermus hydrogenoformans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.6 | - |
(2S,3S)-3-ethylaspartic acid | pH 9, 30°C | Carboxydothermus hydrogenoformans | |
16 | - |
(2S,3S)-3-ethylaspartic acid | pH 9, 70°C | Carboxydothermus hydrogenoformans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | required for maximum activity | Carboxydothermus hydrogenoformans | |
Mg2+ | required for maximum activity | Carboxydothermus hydrogenoformans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49000 | - |
SDS-PAGE | Carboxydothermus hydrogenoformans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Carboxydothermus hydrogenoformans | Q3AEU2 | - |
- |
Purification (Comment) | Organism |
---|---|
using metal affinity chromatography | Carboxydothermus hydrogenoformans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S,3S)-3-ethylaspartic acid | - |
Carboxydothermus hydrogenoformans | ? | - |
? | |
ethylfumarate + NH3 | - |
Carboxydothermus hydrogenoformans | ? | - |
? | |
fumarate + NH3 | - |
Carboxydothermus hydrogenoformans | ? | - |
? | |
mesaconate + ethylamine | - |
Carboxydothermus hydrogenoformans | ? | - |
? | |
mesaconate + hydrazine | - |
Carboxydothermus hydrogenoformans | ? | - |
? | |
mesaconate + hydroxylamine | - |
Carboxydothermus hydrogenoformans | ? | - |
? | |
mesaconate + methoxylamine | - |
Carboxydothermus hydrogenoformans | ? | - |
? | |
mesaconate + methylamine | - |
Carboxydothermus hydrogenoformans | ? | - |
? | |
mesaconate + NH3 | - |
Carboxydothermus hydrogenoformans | ? | - |
? | |
methylfumarate + NH3 | - |
Carboxydothermus hydrogenoformans | ? | - |
? | |
propylfumarate + NH3 | - |
Carboxydothermus hydrogenoformans | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 49000 | Carboxydothermus hydrogenoformans |
Synonyms | Comment | Organism |
---|---|---|
Ch-MAL | - |
Carboxydothermus hydrogenoformans |
methylaspartate ammonia lyase | - |
Carboxydothermus hydrogenoformans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Carboxydothermus hydrogenoformans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
stable for more than 4 hours | Carboxydothermus hydrogenoformans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
16 | - |
(2S,3S)-3-ethylaspartic acid | pH 9, 30°C | Carboxydothermus hydrogenoformans | |
78 | - |
(2S,3S)-3-ethylaspartic acid | pH 9, 70°C | Carboxydothermus hydrogenoformans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Carboxydothermus hydrogenoformans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.9 | - |
(2S,3S)-3-ethylaspartic acid | pH 9, 30°C | Carboxydothermus hydrogenoformans | |
35 | - |
(2S,3S)-3-ethylaspartic acid | pH 9, 70°C | Carboxydothermus hydrogenoformans |