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(2E)-2-butylbut-2-enedioate + NH3
?
wild-type: conversion 0%, mutant L384A: conversion 59%
-
-
r
(2E)-2-ethylbut-2-enedioate + NH3
?
wild-type: conversion 74%, mutant L384A: conversion 76%
-
-
r
(2E)-2-hexylbut-2-enedioate + NH3
?
wild-type: conversion 0%, mutant L384A: conversion 53%
-
-
r
(2E)-2-methylbut-2-enedioate + NH3
?
wild-type: conversion 80%, mutant L384A: conversion 75%
-
-
r
(2E)-2-pentylbut-2-enedioate + NH3
?
wild-type: conversion 0%, mutant L384A: conversion 52%
-
-
r
(2E)-2-phenylbut-2-enedioate + NH3
?
wild-type: conversion 0%, mutant L384A: conversion 90%
-
-
r
(2E)-2-propylbut-2-enedioate + NH3
?
wild-type: conversion 57%, mutant L384A: conversion 67%
-
-
r
(2R,3S)-3-chloroaspartic acid
2-chloro-2-butenedioic acid
(2S)-2-hydrazinosuccinic acid
fumarate + hydrazine
-
-
-
?
(2S)-aspartate
fumarate + NH3
-
-
-
?
(2S)-N,N-dimethylaspartic acid
fumarate + dimethylamine
-
-
-
?
(2S)-N-ethylaspartic acid
fumarate + ethylamine
-
-
-
?
(2S)-N-hydroxyaspartic acid
fumarate + hydroxylamine
-
-
-
?
(2S)-N-methoxyaspartic acid
fumarate + methoxylamine
-
-
-
?
(2S)-N-methylaspartic acid
fumarate + methylamine
-
-
-
?
(2S,3R)-3-ethylaspartic acid
?
(2S,3S)-3-ethylaspartate
?
-
-
-
?
(2S,3S)-3-ethylaspartic acid
?
(2S,3S)-3-methylaspartate
mesaconate + NH3
(2S,3S)-N-hydroxy-3-ethylaspartic acid
ethylfumarate + hydroxylamine
-
-
-
?
(2S,3S)-N-hydroxy-3-methylaspartic acid
mesaconate + hydroxylamine
-
-
-
?
(2S,3S)-N-methoxy-3-methylaspartic acid
mesaconate + methoxylamine
-
-
-
?
(2Z)-2-(benzyloxy)but-2-enedioate + NH3
?
wild-type: conversion 0%, mutant L384A: conversion 60%
-
-
r
(2Z)-2-(benzylsulfanyl)but-2-enedioate + NH3
?
wild-type: conversion 0%, mutant L384A: conversion 42%
-
-
r
(2Z)-2-(ethylsulfanyl)but-2-enedioate + NH3
?
wild-type: conversion 36%, mutant L384A: conversion 50%
-
-
r
(2Z)-2-(phenylsulfanyl)but-2-enedioate + NH3
?
wild-type: conversion 0%, mutant L384A: conversion 34%
-
-
r
(2Z)-2-ethoxybut-2-enedioate + NH3
?
wild-type: conversion 46%, mutant L384A: conversion 43%
-
-
r
(2Z)-2-phenoxybut-2-enedioate + NH3
?
wild-type: conversion 0%, mutant L384A: conversion 44%
-
-
r
(S)-aspartate
fumarate + NH3
-
-
-
?
2-hydrazino-3-chlorosuccinic acid
chlorofumarate + hydrazine
-
-
-
?
2-hydrazino-3-ethylsuccinic acid
ethylfumarate + hydrazine
-
-
-
?
2-hydrazino-3-isopropylsuccinic acid
isopropylfumarate + hydrazine
-
-
-
?
2-hydrazino-3-propylsuccinic acid
propylfumarate + hydrazine
-
-
-
?
3-bromoaspartate
bromofumarate + NH3
3-chloroaspartate
chlorofumarate + NH3
3-fluoroaspartate
fluorofumarate + NH3
-
-
not a good substrate
?
beta-isopropylaspartate
?
-
-
-
-
?
erythro-3-methylaspartate
?
hydrazino-3-methylsuccinic acid
mesaconate + hydrazine
-
-
-
?
iso-propylfumarate + NH3
?
-
-
-
-
?
L-aspartate
fumarate + NH3
L-erythro-3-methylaspartate
mesaconate + NH3
L-threo-3-methylaspartate
?
L-threo-3-methylaspartate
mesaconate + NH3
L-threo-3-methylaspartic acid
mesaconate + NH3
assay at pH 9.0, 30°C
-
-
?
mesaconate + (aminooxy)ethane
?
mutant Q73A, conversion: 100%, yield: 61%
-
-
r
mesaconate + (aminooxy)methane
?
mutant Q73A, conversion: 99%, excess of threo isomer over erythro isomer: more than 95%
-
-
r
mesaconate + 1-cyclopropylmethanamine
?
mesaconate + 2-aminoethanol
?
mutant Q73A, conversion: 37%, excess of threo isomer over erythro isomer: more than 95%
-
-
r
mesaconate + 2-methoxyethanamine
?
mesaconate + 3-aminopropan-1-ol
?
mesaconate + aminobutane
?
mesaconate + aniline
?
mutant Q73A, conversion: 6%, excess of threo isomer over erythro isomer: more than 95%
-
-
r
mesaconate + cyclobutanamine
?
mesaconate + cyclohexanamine
?
mutant Q73A, conversion: 21%, excess of threo isomer over erythro isomer: more than 95%
-
-
r
mesaconate + cyclopentanamine
?
mutant Q73A, conversion: 36%, excess of threo isomer over erythro isomer: more than 95%
-
-
r
mesaconate + cyclopropanamine
?
mesaconate + ethanamine
?
mesaconate + ethane-1,2-diamine
?
mesaconate + ethylamine
?
-
-
-
?
mesaconate + hexanamine
?
mutant Q73A, conversion: 17%, excess of threo isomer over erythro isomer: more than 95%
-
-
r
mesaconate + hydrazine
?
-
-
-
?
mesaconate + hydroxylamine
?
-
-
-
?
mesaconate + methanamine
?
mesaconate + methoxylamine
?
-
-
-
?
mesaconate + methylamine
?
-
-
-
?
mesaconate + N-methylethane-1,2-diamine
?
mutant Q73A, conversion: 80%, excess of threo isomer over erythro isomer: more than 95%
-
-
r
mesaconate + NH3
?
-
-
-
?
mesaconate + NH3
L-threo-3-methylaspartate
mesaconate + NH3
L-threo-3-methylaspartate + L-erythro-3-methylaspartate
conversion 2h: 73% (wild-type), diastereomeric product ratio (threo:erythro) after 2h: 89:11
-
-
r
mesaconate + NH4Cl
methylaspartate + HCl
-
-
-
-
r
mesaconate + pentanamine
?
mesaconate + propan-2-amine
?
mutant Q73A, conversion: 8%, excess of threo isomer over erythro isomer: more than 95%, enantiomeric excess: more than 99%
-
-
r
mesaconate + propanamine
?
mesaconate + propane-1,3-diamine
?
mesaconic acid + NH3
L-threo-3-methylaspartate
assay at pH 9.0, 30°C
-
-
r
methylfumarate + NH3
?
-
-
-
?
N,3-dimethylaspartic acid
methylfumarate + methylamine
-
-
-
?
N-methyl-3-ethylaspartic acid
ethylfumarate + methylamine
-
-
-
?
NH3 + (2E)-2-methylbut-2-enedioate
(2S,3R)-3-methylaspartate
-
-
minor product
r
NH3 + (2E)-2-methylbut-2-enedioate
(2S,3S)-3-methylaspartate
-
-
major product
r
propylfumarate + NH3
?
-
-
-
?
additional information
?
-
(2R,3S)-3-chloroaspartic acid
2-chloro-2-butenedioic acid
-
-
-
-
?
(2R,3S)-3-chloroaspartic acid
2-chloro-2-butenedioic acid
-
-
-
-
?
(2S,3R)-3-ethylaspartic acid
?
-
-
-
-
?
(2S,3R)-3-ethylaspartic acid
?
-
-
-
-
?
(2S,3R)-3-ethylaspartic acid
?
-
-
-
-
?
(2S,3S)-3-ethylaspartic acid
?
Bacterium cadaveris
-
-
-
-
?
(2S,3S)-3-ethylaspartic acid
?
-
-
-
?
(2S,3S)-3-ethylaspartic acid
?
-
-
-
-
?
(2S,3S)-3-ethylaspartic acid
?
-
-
-
-
?
(2S,3S)-3-ethylaspartic acid
?
-
-
-
-
?
(2S,3S)-3-ethylaspartic acid
?
-
-
-
-
?
(2S,3S)-3-methylaspartate
mesaconate + NH3
-
-
-
-
r
(2S,3S)-3-methylaspartate
mesaconate + NH3
-
-
-
-
r
3-bromoaspartate
bromofumarate + NH3
Bacterium cadaveris
-
-
-
?
3-bromoaspartate
bromofumarate + NH3
-
-
-
?
3-bromoaspartate
bromofumarate + NH3
-
-
during reaction, enzyme becomes inactive. This is reduced in the presence of alternative substrates
?
3-chloroaspartate
chlorofumarate + NH3
-
(2R,3S)-3-chloroaspartic acid
-
?
3-chloroaspartate
chlorofumarate + NH3
-
(2R,3S)-3-chloroaspartic acid
-
?
3-chloroaspartate
chlorofumarate + NH3
-
(2R,3S)-3-chloroaspartic acid
-
?
3-chloroaspartate
chlorofumarate + NH3
-
-
-
?
3-chloroaspartate
chlorofumarate + NH3
-
-
-
?
3-chloroaspartate
chlorofumarate + NH3
-
-
-
?
3-chloroaspartate
chlorofumarate + NH3
-
-
-
?
3-chloroaspartate
chlorofumarate + NH3
-
-
-
?
3-chloroaspartate
chlorofumarate + NH3
-
-
-
?
3-chloroaspartate
chlorofumarate + NH3
-
-
-
?
3-chloroaspartate
chlorofumarate + NH3
-
(2R,3S)-3-chloroaspartic acid
-
?
3-chloroaspartate
chlorofumarate + NH3
-
(2R,3S)-3-chloroaspartic acid
-
?
? + NH4Cl
?
-
-
-
-
?
beta-ethylaspartate
?
Bacterium cadaveris
-
-
-
-
?
beta-ethylaspartate
?
-
-
-
-
?
beta-ethylaspartate
?
-
3-ethylaspartic acid
-
-
?
beta-ethylaspartate
?
-
3-ethylaspartate
-
-
?
beta-ethylaspartate
?
-
-
-
-
?
beta-ethylaspartate
?
-
3-ethylaspartic acid
-
-
?
beta-propylaspartate
?
-
-
-
-
?
beta-propylaspartate
?
-
beta-n-propylaspartate
-
-
?
erythro-3-methylaspartate
?
Bacterium cadaveris
-
-
-
-
?
erythro-3-methylaspartate
?
Bacterium cadaveris
-
(2S,3R)-3-methylaspartic acid
-
-
?
erythro-3-methylaspartate
?
-
-
-
-
?
erythro-3-methylaspartate
?
-
erythro-beta-methyl-L-aspartate
-
-
?
erythro-3-methylaspartate
?
-
(2S,3R)-3-methylaspartic acid
-
-
?
erythro-3-methylaspartate
?
-
erythro-3-methyl-L-aspartate
-
-
?
erythro-3-methylaspartate
?
-
erythro-beta-methylaspartate
-
-
?
erythro-3-methylaspartate
?
-
-
-
-
?
erythro-3-methylaspartate
?
-
erythro-3-methyl-L-aspartate
-
-
?
erythro-3-methylaspartate
?
-
erythro-beta-methylaspartate
-
-
?
erythro-3-methylaspartate
?
-
-
-
-
?
erythro-3-methylaspartate
?
-
(2S,3R)-3-methylaspartic acid
-
-
?
ethylfumarate + NH3
?
-
-
-
?
ethylfumarate + NH3
?
-
-
-
-
?
ethylfumarate + NH3
?
-
-
-
-
?
ethylfumarate + NH3
?
-
-
-
-
?
ethylfumarate + NH3
?
-
-
-
-
?
ethylfumarate + NH3
?
-
-
-
-
?
ethylfumarate + NH3
?
-
-
-
-
?
ethylfumarate + NH3
?
-
-
-
-
?
ethylfumarate + NH3
?
-
-
-
-
?
L-aspartate
fumarate + NH3
Bacterium cadaveris
-
(2S,3R) [3-3H]aspartic acid
-
-
?
L-aspartate
fumarate + NH3
-
-
-
-
?
L-aspartate
fumarate + NH3
-
rate of deamination 100 times slower than that of L-threo-3-methylaspartate
-
?
L-aspartate
fumarate + NH3
-
(2S,3R) [3-3H]aspartic acid
-
-
?
L-aspartate
fumarate + NH3
-
(2S,3R) [3-3H]aspartic acid
amination 1.8 times faster than the amination of mesaconate
?
L-aspartate
fumarate + NH3
-
(2S)-aspartate
-
?
L-aspartate
fumarate + NH3
-
(2S)-aspartate
-
-
?
L-aspartate
fumarate + NH3
-
(2S)-aspartate
amination 1.8 times faster than the amination of mesaconate
?
L-aspartate
fumarate + NH3
-
(2S)-aspartate
-
-
?
L-aspartate
fumarate + NH3
-
-
-
-
?
L-aspartate
fumarate + NH3
-
rate of deamination 100 times slower than that of L-threo-3-methylaspartate
-
?
L-aspartate
fumarate + NH3
-
(2S,3R) [3-3H]aspartic acid
amination 1.8 times faster than the amination of mesaconate
?
L-aspartate
fumarate + NH3
-
(2S)-aspartate
amination 1.8 times faster than the amination of mesaconate
?
L-aspartate
fumarate + NH3
-
(2S)-aspartate
-
-
?
L-erythro-3-methylaspartate
mesaconate + NH3
-
i.e. (2S,3R)-3-methylaspartate, slow syn elimination
-
?
L-erythro-3-methylaspartate
mesaconate + NH3
-
i.e. (2S,3R)-3-methylaspartate, slow syn elimination
-
?, r
L-threo-3-methylaspartate
?
Bacterium cadaveris
-
-
-
-
?
L-threo-3-methylaspartate
?
-
-
-
-
?
L-threo-3-methylaspartate
?
-
-
-
-
?
L-threo-3-methylaspartate
?
-
-
-
-
?
L-threo-3-methylaspartate
?
-
-
-
-
?
L-threo-3-methylaspartate
?
-
-
-
-
?
L-threo-3-methylaspartate
?
-
-
-
-
?
L-threo-3-methylaspartate
?
-
-
-
-
?
L-threo-3-methylaspartate
?
-
-
-
-
?
L-threo-3-methylaspartate
?
-
-
-
-
?
L-threo-3-methylaspartate
?
-
-
-
-
?
L-threo-3-methylaspartate
?
-
-
5670, 5997, 6049, 6050, 6051, 6053, 6054, 6055, 6056, 6057, 6058, 6059, 6060, 6062, 6064, 6065, 6066, 6067 -
-
?
L-threo-3-methylaspartate
?
-
-
-
-
?
L-threo-3-methylaspartate
?
-
-
-
-
?
L-threo-3-methylaspartate
?
-
-
-
-
?
L-threo-3-methylaspartate
?
-
-
-
-
?
L-threo-3-methylaspartate
?
-
-
-
-
?
L-threo-3-methylaspartate
?
-
-
-
-
?
L-threo-3-methylaspartate
?
-
-
-
-
?
L-threo-3-methylaspartate
?
-
-
-
-
?
L-threo-3-methylaspartate
?
-
-
-
-
?
L-threo-3-methylaspartate
?
-
-
-
-
?
L-threo-3-methylaspartate
mesaconate + NH3
Bacterium cadaveris
-
r
-
?
L-threo-3-methylaspartate
mesaconate + NH3
Bacterium cadaveris
-
r
-
?
L-threo-3-methylaspartate
mesaconate + NH3
Bacterium cadaveris
-
r
-
?
L-threo-3-methylaspartate
mesaconate + NH3
Bacterium cadaveris
-
r
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
-
-
r
L-threo-3-methylaspartate
mesaconate + NH3
-
r
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
r
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
r
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
r
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
r
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
r
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
r
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
r
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
r
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
r
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
r
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
r
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
r
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
r
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
r
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
-
-
r
L-threo-3-methylaspartate
mesaconate + NH3
-
-
-
r
L-threo-3-methylaspartate
mesaconate + NH3
-
-
-
r
L-threo-3-methylaspartate
mesaconate + NH3
-
-
-
r
L-threo-3-methylaspartate
mesaconate + NH3
-
-
-
r
L-threo-3-methylaspartate
mesaconate + NH3
-
r
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
r
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
r
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
r
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
r
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
r
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
r
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
r
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
r
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
r
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
r
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
r
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
r
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
r
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
r
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
r
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
r
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
r
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
threo-beta-methyl-L-aspartate
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
threo-beta-methyl-L-aspartate
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
threo-beta-methyl-L-aspartate
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
beta-methylaspartate
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
i.e. (2S,3S)-3-methylaspartate
-
r
L-threo-3-methylaspartate
mesaconate + NH3
-
i.e. (2S,3S)-3-methylaspartate
-
r
L-threo-3-methylaspartate
mesaconate + NH3
the enzyme is part of a catabolic pathway for L-glutamate
-
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
-
-
r
L-threo-3-methylaspartate
mesaconate + NH3
-
r
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
i.e. (2S,3S)-3-methylaspartate
-
r
L-threo-3-methylaspartate
mesaconate + NH3
-
-
-
r
L-threo-3-methylaspartate
mesaconate + NH3
-
r
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
r
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
threo-beta-methyl-L-aspartate
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
r
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
r
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
r
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
r
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
r
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
r
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
r
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
r
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
r
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
-
-
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
-
-
r
L-threo-3-methylaspartate
mesaconate + NH3
-
r
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
r
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
r
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
r
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
r
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
r
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
r
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
r
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
r
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
mesaconate + 1-cyclopropylmethanamine
?
mutant Q73A, conversion: 61%, excess of threo isomer over erythro isomer: more than 95%, enantiomeric excess: more than 99%
-
-
r
mesaconate + 1-cyclopropylmethanamine
?
mutant Q73A, conversion: 96%, yield: 28%, enantiomeric excess: more than 99%
-
-
r
mesaconate + 2-methoxyethanamine
?
mutant Q73A, conversion: 100%, yield: 53%
-
-
r
mesaconate + 2-methoxyethanamine
?
mutant Q73A, conversion: 60%, excess of threo isomer over erythro isomer: more than 95%
-
-
r
mesaconate + 3-aminopropan-1-ol
?
mutant Q73A, conversion: 56%, excess of threo isomer over erythro isomer: 80%
-
-
r
mesaconate + 3-aminopropan-1-ol
?
mutant Q73A, conversion: 99%, yield: 57%
-
-
r
mesaconate + aminobutane
?
mutant Q73A, conversion: 60%, excess of threo isomer over erythro isomer: more than 95%, enantiomeric excess: more than 99%
-
-
r
mesaconate + aminobutane
?
mutant Q73A, conversion: 97%, yield: 33%, enantiomeric excess: more than 99%
-
-
r
mesaconate + cyclobutanamine
?
mutant Q73A, conversion: 65%, excess of threo isomer over erythro isomer: more than 95%, enantiomeric excess: more than 99%
-
-
r
mesaconate + cyclobutanamine
?
mutant Q73A, conversion: 99%, yield: 74%, enantiomeric excess: more than 99%
-
-
r
mesaconate + cyclopropanamine
?
mutant Q73A, conversion: 100%, yield: 48%
-
-
r
mesaconate + cyclopropanamine
?
mutant Q73A, conversion: 75%, excess of threo isomer over erythro isomer: more than 95%
-
-
r
mesaconate + ethanamine
?
mutant Q73A, conversion: 100%, yield: 75%, enantiomeric excess: more than 99%
-
-
r
mesaconate + ethanamine
?
mutant Q73A, conversion: 51%, excess of threo isomer over erythro isomer: more than 95%, enantiomeric excess: more than 99%
-
-
r
mesaconate + ethane-1,2-diamine
?
mutant Q73A, conversion: 90%, excess of threo isomer over erythro isomer: more than 95%
-
-
r
mesaconate + ethane-1,2-diamine
?
mutant Q73A, conversion: 99%, yield: 67%
-
-
r
mesaconate + methanamine
?
mutant Q73A, conversion: 100%, yield: 96%, enantiomeric excess: more than 99%
-
-
r
mesaconate + methanamine
?
mutant Q73A, conversion: 74%, excess of threo isomer over erythro isomer: more than 95%
-
-
r
mesaconate + NH3
L-threo-3-methylaspartate
-
-
-
r
mesaconate + NH3
L-threo-3-methylaspartate
mutant Q73A, conversion: 100%, yield: 79%, enantiomeric excess: more than 99%
-
-
r
mesaconate + NH3
L-threo-3-methylaspartate
mutant Q73A, conversion: 79%, excess of threo isomer over erythro isomer: 5%
-
-
r
mesaconate + NH3
L-threo-3-methylaspartate
wild-type: conversion 100%, mutant L384A: conversion 99%
-
-
r
mesaconate + pentanamine
?
mutant Q73A, conversion: 29%, excess of threo isomer over erythro isomer: more than 95%
-
-
r
mesaconate + pentanamine
?
mutant Q73A, conversion: 91%, yield: 14%, enantiomeric excess: more than 99%
-
-
r
mesaconate + propanamine
?
mutant Q73A, conversion: 53%, excess of threo isomer over erythro isomer: more than 95%, enantiomeric excess: more than 99%
-
-
r
mesaconate + propanamine
?
mutant Q73A, conversion: 99%, yield: 53%, enantiomeric excess: more than 99%
-
-
r
mesaconate + propane-1,3-diamine
?
mutant Q73A, conversion: 100%, yield: 61%
-
-
r
mesaconate + propane-1,3-diamine
?
mutant Q73A, conversion: 78%, excess of threo isomer over erythro isomer: more than 95%
-
-
r
additional information
?
-
Bacterium cadaveris
-
-
-
-
?
additional information
?
-
-
-
-
-
?
additional information
?
-
-
no reaction with aspartic acid
-
-
?
additional information
?
-
-
-
-
-
?
additional information
?
-
-
no reaction with aspartic acid
-
-
?
additional information
?
-
-
-
-
-
?
additional information
?
-
-
-
-
-
?
additional information
?
-
-
-
-
-
?
additional information
?
-
-
-
-
-
?
additional information
?
-
-
enzyme is specific for the L-configuration
-
-
?
additional information
?
-
-
catalytic rates for the enzymatic amination of fumarate and mesaconate are faster than the rates of the corresponding deamination reactions
-
-
?
additional information
?
-
-
enzyme is involved in the catabolic pathway for glutamate
-
-
?
additional information
?
-
-
enzyme is involved in the catabolic pathway for glutamate
-
-
?
additional information
?
-
-
enzyme is associated with an anaerobic degradation of the (S)-glutamic acid in obligat anaerobes
-
-
?
additional information
?
-
-
enzyme is involved in the catabolic pathway for glutamate
-
-
?
additional information
?
-
-
enzyme is involved in the catabolic pathway for glutamate
-
-
?
additional information
?
-
-
-
-
-
?
additional information
?
-
-
-
-
-
?
additional information
?
-
-
-
-
-
?
additional information
?
-
-
enzyme is associated with an anaerobic degradation of the (S)-glutamic acid in obligat anaerobes
-
-
?
additional information
?
-
-
-
-
-
?
additional information
?
-
-
ammonia is the natural nucleophile for the enzyme
-
-
?
additional information
?
-
-
-
-
-
?
additional information
?
-
-
-
-
-
?
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Kato, Y.; Asano, Y.
3-Methylaspartate ammonia-lyase as a marker enzyme of the mesaconate pathway for (S)-glutamate fermentation in Enterobacteriaceae
Arch. Microbiol.
168
457-463
1997
Citrobacter amalonaticus, Citrobacter freundii, Citrobacter koseri, Clostridium tetanomorphum, Raoultella planticola, Morganella morganii, Citrobacter amalonaticus YG-1002, Citrobacter freundii YG-0504, Morganella morganii NCIMB 232, Raoultella planticola IAM 1133, Citrobacter freundii TPU 0011, Citrobacter koseri JCM 1659, Morganella morganii NCIMB 9525, Citrobacter koseri JCM 1658, Morganella morganii YG-0601, Morganella morganii NCIMB 10466
brenda
Hanson, K.R.; Havir, E.A.
The enzymic elimination of ammonia
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
7
75-166
1972
Bacterium cadaveris, Clostridium tetanomorphum, Clostridium tetanomorphum H1 / ATCC 15920
-
brenda
Bright, H.J.; Ingraham, L.L.
The preparation of crystalline beta-methylaspartase
Biochim. Biophys. Acta
44
586-588
1960
Clostridium tetanomorphum
-
brenda
Bright, H.J.
Divalent metal activation of beta-methylaspartase. The importance of ionic radius
Biochemistry
6
1191-1203
1967
Clostridium tetanomorphum
brenda
Hsiang, M.W.; Bright, H.J.
beta-methylaspartase from Clostridium tetanomorphum
Methods Enzymol.
13
347-353
1969
Bacterium cadaveris, Clostridium tetanomorphum, Clostridium tetanomorphum H1 / ATCC 15920
-
brenda
Akhtar, M.; Cohen, M.A.; Gani, D.
Enzymic synthesis of 3-halogenoaspartic acids using beta-methylaspartase: Inhibition by 3-bromoaspartic acid
J. Chem. Soc. Chem. Commun.
1986
1290-1291
1980
Clostridium tetanomorphum
-
brenda
Akhtar, M.; Botting, N.P.; Cohen, M.A.; Gani, D.
Enantiospecific synthesis of 3-substituted aspartic acids via enzymic amination of substituted fumaric acids
Tetrahedron
43
5899-5908
1987
Clostridium tetanomorphum
-
brenda
Botting, N.P.; Akhtar, M.; Cohen, M.A.; Gani, D.
Substrate specificity of the 3-methylaspartate ammonia-lyase reaction: Observation of differential relative reaction rates for substrate-product pairs
Biochemistry
27
2953-2955
1988
Clostridium tetanomorphum, Clostridium tetanomorphum H1 / ATCC 15920
brenda
Botting, N.P.; Cohen, M.A.; Akhtar, M.; Gani, D.
Primary deuterium isotope effects for the 3-methylaspartase-catalyzed deamination of (2S)-aspartic acid, (2S,3S)-3-methylaspartic acid, and (2S,3S)-3-ethylaspartic acid
Biochemistry
27
2956-2959
1988
Clostridium tetanomorphum, Clostridium tetanomorphum H1 / ATCC 15920
brenda
Goda, S.K.; Minton, N.P.; Botting, N.P.; Gani, D.
Cloning, sequencing, and expression in Escherichia coli of the Clostridium tetanomorphum gene encoding beta-methylaspartase and characterization of the recombinant protein
Biochemistry
31
10747-10756
1992
Clostridium tetanomorphum, Clostridium tetanomorphum H1 / ATCC 15920
brenda
Botting, N.P.; Gani, D.
Mechanism of C-3 hydrogen exchange and the elimination of ammonia in the 3-methylaspartate ammonia-lyase reaction
Biochemistry
31
1509-1520
1992
Clostridium tetanomorphum, Clostridium tetanomorphum H1 / ATCC 15920
brenda
Kato, Y.; Asano, Y.
3-Methylaspartate ammonia-lyase from a facultative anaerobe, strain YG-1002
Appl. Microbiol. Biotechnol.
43
901-907
1995
Citrobacter amalonaticus, Clostridium tetanomorphum, Citrobacter amalonaticus YG-1002, Clostridium tetanomorphum H1 / ATCC 15920, Clostridium tetanomorphum NCIMB 11547
brenda
Kato, Y.; Asano, Y.
Purification and properties of crystalline 3-methylaspartase from two facultative anaerobes, Citrobacter sp. strain YG-0504 and Morganella morganii strain YG-0601
Biosci. Biotechnol. Biochem.
59
93-99
1995
Citrobacter freundii, Clostridium tetanomorphum, Morganella morganii, Clostridium tetanomorphum NCIMB 11547, Morganella morganii YG-0601
brenda
Gulzar, M.S.; Akhtar, M.; Gani, D.
Preparation of N-substituted aspartic acids via enantiospecific conjugate addition of N-nucleophiles to fumaric acids using methylaspartase: synthetic utility and mechanistic implications
J. Chem. Soc. Perkin Trans. I
1197
649-655
1997
Escherichia coli
-
brenda
Kato, Y.; Asano, Y.
Cloning, nucleotide sequencing, and expression of the 3-methylaspartate ammonia-lyase gene from Citrobacter amalonaticus strain YG-1002
Appl. Microbiol. Biotechnol.
50
468-474
1998
Citrobacter amalonaticus, Clostridium tetanomorphum, Citrobacter amalonaticus YG-1002, Citrobacter amalonaticus TPU 6323, Clostridium tetanomorphum NCIMB 11547
brenda
Gani, D.; Archer, C.H.; Botting, N.P.; Pollard, J.R.
The methylaspartase reaction probed using 2H- and 15N-isotope E ects for three substrates: A flip from a concerted to a carbocationic amino-enzyme elimination mechanism upon changing the C-3 stereochemistry in the substrate from R to S
Bioorg. Med. Chem.
7
977-990
1999
Clostridium tetanomorphum, Clostridium tetanomorphum H1 / ATCC 15920
brenda
Winkler, M.F.; Williams, V.R.
New substrates for beta-methylaspartase
Biochim. Biophys. Acta
146
287-289
1967
Clostridium tetanomorphum
brenda
Botting, N.P.; Akhtar, M.; Cohen, M.A.; Gani, D.
Mechanism of the enzymic elimination of ammonia from 3-substituted aspartic acids by 3-methylaspartase
J. Chem. Soc. Chem. Commun.
1987
1371-1373
1987
Bacterium cadaveris, Clostridium tetanomorphum
-
brenda
Akhtar, M.; Cohen, M.A.; Gani, D.
Stereochemical course of the enzymic amination of chloro- and bromo acid by 3-methylaspartate ammoni-lyase
Tetrahedron Lett.
28
2413-2416
1987
Bacterium cadaveris, Clostridium tetanomorphum
-
brenda
Asuncion, M.; Barlow, J.N.; Pollard, J.; Staines, A.G.; McMahon, S.A.; Blankenfeldt, W.; Gani, D.; Naismith, J.H.
Overexpression, purification, crystallization and data collection of 3-methylaspartase from Clostridium tetanomorphum
Acta Crystallogr. Sect. D
57
731-733
2001
Clostridium tetanomorphum
brenda
Pollard, J.R.; Richardson, S.; Akhtar, M.; Lasry, P.; Neal, T.; Botting, N.P.; Gani, D.
Mechanism of 3-methylaspartase probed using deuterium and solvent isotope effects and active-site directed reagents: identification of an essential cysteine residue
Bioorg. Med. Chem.
7
949-975
1999
Clostridium tetanomorphum, Clostridium tetanomorphum H1 / ATCC 15920
brenda
Asuncion, M.; Blankenfeldt, W.; Barlow, J.N.; Gani, D.; Naismith, J.H.
The structure of 3-methylaspartase from Clostridium tetanomorphum functions via the common enolase chemical step
J. Biol. Chem.
277
8306-8311
2002
Clostridium tetanomorphum
brenda
Bearne, S.L.; White, R.L.; MacDonnell, J.E.; Bahrami, S.; Gronlund, J.
Purification and characterization of beta-methylaspartase from Fusobacterium varium
Mol. Cell. Biochem.
221
117-126
2001
Fusobacterium varium
brenda
Levy, C.W.; Buckley, P.A.; Sedelnikova, S.; Kato, Y.; Asano, Y.; Rice, D.W.; Baker, P.J.
Insights into enzyme evolution revealed by the structure of methylaspartate ammonia lyase
Structure
10
105-113
2002
Citrobacter amalonaticus
brenda
Asano, Y.; Kato, Y.; Levy, C.; Baker, P.; Rice, D.
Structure and Function of Amino Acid Ammonia-lyases
Biocatal. Biotransform.
22
131-138
2004
Citrobacter amalonaticus, Citrobacter freundii, Clostridium tetanomorphum, Morganella morganii, Citrobacter amalonaticus YG-1002, Clostridium tetanomorphum H1 / ATCC 15920, Citrobacter freundii YG.0504, Morganella morganii YG-0601
-
brenda
Potrykus, J.; Mahaney, B.; White, R.L.; Bearne, S.L.
Proteomic investigation of glucose metabolism in the butyrate-producing gut anaerobe Fusobacterium varium
Proteomics
7
1839-1853
2007
Fusobacterium varium
brenda
Lee, H.; Yoon, M.; Marsh, E.N.
Synthesis of mono- and di-deuterated (2S,3S)-3-methylaspartic acids to facilitate measurement of intrinsic kinetic isotope effects in enzymes
Tetrahedron
63
4663-4668
2007
Escherichia coli
brenda
Raj, H.; Weiner, B.; Veetil, V.P.; Reis, C.R.; Quax, W.J.; Janssen, D.B.; Feringa, B.L.; Poelarends, G.J.
Alteration of the diastereoselectivity of 3-methylaspartate ammonia lyase by using structure-based mutagenesis
ChemBioChem
10
2236-2245
2009
Clostridium tetanomorphum (Q05514)
brenda
Ramezani, M.; Resmer, K.L.; White, R.L.
Glutamate racemization and catabolism in Fusobacterium varium
FEBS J.
278
2540-2551
2011
Fusobacterium varium
brenda
Raj, H.; Puthan Veetil, V.; Szymanski, W.; Dekker, F.J.; Quax, W.J.; Feringa, B.L.; Janssen, D.B.; Poelarends, G.J.
Characterization of a thermostable methylaspartate ammonia lyase from Carboxydothermus hydrogenoformans
Appl. Microbiol. Biotechnol.
94
385-397
2012
Carboxydothermus hydrogenoformans (Q3AEU2), Carboxydothermus hydrogenoformans
brenda
PuthanVeetil, V.; Raj, H.; DeVilliers, M.; Tepper, P.; Dekker, F.; Quax, W.; Poelarends, G.
Enantioselective Synthesis of N-Substituted Aspartic Acids Using an Engineered Variant of Methylaspartate Ammonia Lyase
ChemCatChem
5
1325-1327
2013
Clostridium tetanomorphum (Q05514)
-
brenda
Raj, H.; Poelarends, G.J.
The roles of active site residues in the catalytic mechanism of methylaspartate ammonia-lyase
FEBS open bio
3
285-290
2013
Clostridium tetanomorphum (Q05514), Clostridium tetanomorphum
brenda
Raj, H.; Szymanski, W.; de Villiers, J.; Rozeboom, H.J.; Veetil, V.P.; Reis, C.R.; de Villiers, M.; Dekker, F.J.; de Wildeman, S.; Quax, W.J.; Thunnissen, A.M.; Feringa, B.L.; Janssen, D.B.; Poelarends, G.J.
Engineering methylaspartate ammonia lyase for the asymmetric synthesis of unnatural amino acids
Nat. Chem.
4
478-484
2012
Clostridium tetanomorphum (Q05514)
brenda
Abidin, M.; Saravanan, T.; Zhang, J.; Tepper, P.; Strauss, E.; Poelarends, G.
Modular enzymatic cascade synthesis of vitamin B5 and its derivatives
Chemistry
24
17434-17438
2018
Clostridium tetanomorphum (Q05514)
brenda