KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
45 | - |
L-serine | pH 8.3, 37°C | Rattus norvegicus | |
50 | - |
L-serine | pH 8.3, 37°C | Homo sapiens | |
57 | - |
L-threonine | pH 8.3, 37°C | Rattus norvegicus | |
57 | - |
L-threonine | pH 8.3, 37°C | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
2 * 33000, SDS-PAGE, 2 * 34702, calculated | Homo sapiens |
34702 | - |
2 * 33000, SDS-PAGE, 2 * 34702, calculated | Homo sapiens |
60000 | - |
gel filtration | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P20132 | - |
- |
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein expressed in Escherichia coli | Rattus norvegicus |
recombinant protein expressed in Escherichia coli | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | confined to periportal region | Rattus norvegicus | - |
liver | predominantly localized in perivenous region | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
96 | - |
substrate L-threonine, pH 8.3, 37°C | Homo sapiens |
116 | - |
substrate L-threonine, pH 8.3, 37°C | Rattus norvegicus |
137 | - |
substrate L-serine, pH 8.3, 37°C | Homo sapiens |
200 | - |
substrate L-serine, pH 8.3, 37°C | Rattus norvegicus |
Storage Stability | Organism |
---|---|
-80°C, stable for more than 2 weeks | Homo sapiens |
0°C, stable for at least 2 weeks | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine | - |
Rattus norvegicus | pyruvate + NH3 | - |
? | |
L-serine | - |
Homo sapiens | pyruvate + NH3 | - |
? | |
L-threonine | - |
Rattus norvegicus | 2-oxobutyrate + NH3 | - |
? | |
L-threonine | - |
Homo sapiens | 2-oxobutyrate + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 33000, SDS-PAGE, 2 * 34702, calculated | Homo sapiens |