BRENDA - Enzyme Database show
show all sequences of 4.3.1.12

Ornithine cyclase (deaminating). II. Properties of the homogeneous enzyme

Muth, W.L.; Costilow, R.N.; J. Biol. Chem. 249, 7457-7462 (1974)

Data extracted from this reference:

General Stability
General Stability
Organism
ornithine, NAD+ and dithiothreitol stabilize
Clostridium sporogenes
Inhibitors
Inhibitors
Commentary
Organism
Structure
iodoacetate
-
Clostridium sporogenes
N-ethylmaleimide
2 mM, complete inhibition
Clostridium sporogenes
O2
-
Clostridium sporogenes
p-chloromercuribenzoate
1 mM, complete inhibition
Clostridium sporogenes
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.5
-
L-ornithine
in the absence of ADP
Clostridium sporogenes
6.2
-
L-ornithine
in the presence of ADP
Clostridium sporogenes
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
41500
-
2 * 41500, SDS-PAGE
Clostridium sporogenes
80220
-
amino acid analysis
Clostridium sporogenes
81000
-
equilibrium sedimentation
Clostridium sporogenes
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-ornithine
Clostridium sporogenes
-
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Clostridium sporogenes
-
ATCC 7955; National Canners Association, PA 3679
-
Oxidation Stability
Oxidation Stability
Organism
addition of dithiothreitol and subsequent incubation in argon atmosphere completely reverses the inhibition caused by oxygen
Clostridium sporogenes
enzyme is sensitive to either pure oxygen or air
Clostridium sporogenes
Purification (Commentary)
Commentary
Organism
-
Clostridium sporogenes
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
additional information
-
-
Clostridium sporogenes
6.84
-
-
Clostridium sporogenes
Storage Stability
Storage Stability
Organism
4░C, 50% loss of activity under argon atmosphere within 3 days
Clostridium sporogenes
stable for at least 1 year when stored in sealed ampoules in argon atmosphere in liquid nitrogen
Clostridium sporogenes
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-ornithine
anaerobic
34265
Clostridium sporogenes
L-proline + NH3
-
34265
Clostridium sporogenes
-
L-ornithine
-
34265
Clostridium sporogenes
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
dimer
2 * 41500, SDS-PAGE
Clostridium sporogenes
Cofactor
Cofactor
Commentary
Organism
Structure
ADP
stimulates
Clostridium sporogenes
ATP
stimulates
Clostridium sporogenes
NAD+
1 mol per mol of enzyme; tightly bound
Clostridium sporogenes
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ADP
stimulates
Clostridium sporogenes
ATP
stimulates
Clostridium sporogenes
NAD+
1 mol per mol of enzyme; tightly bound
Clostridium sporogenes
General Stability (protein specific)
General Stability
Organism
ornithine, NAD+ and dithiothreitol stabilize
Clostridium sporogenes
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
iodoacetate
-
Clostridium sporogenes
N-ethylmaleimide
2 mM, complete inhibition
Clostridium sporogenes
O2
-
Clostridium sporogenes
p-chloromercuribenzoate
1 mM, complete inhibition
Clostridium sporogenes
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.5
-
L-ornithine
in the absence of ADP
Clostridium sporogenes
6.2
-
L-ornithine
in the presence of ADP
Clostridium sporogenes
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
41500
-
2 * 41500, SDS-PAGE
Clostridium sporogenes
80220
-
amino acid analysis
Clostridium sporogenes
81000
-
equilibrium sedimentation
Clostridium sporogenes
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-ornithine
Clostridium sporogenes
-
?
-
-
-
Oxidation Stability (protein specific)
Oxidation Stability
Organism
addition of dithiothreitol and subsequent incubation in argon atmosphere completely reverses the inhibition caused by oxygen
Clostridium sporogenes
enzyme is sensitive to either pure oxygen or air
Clostridium sporogenes
Purification (Commentary) (protein specific)
Commentary
Organism
-
Clostridium sporogenes
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
additional information
-
-
Clostridium sporogenes
6.84
-
-
Clostridium sporogenes
Storage Stability (protein specific)
Storage Stability
Organism
4░C, 50% loss of activity under argon atmosphere within 3 days
Clostridium sporogenes
stable for at least 1 year when stored in sealed ampoules in argon atmosphere in liquid nitrogen
Clostridium sporogenes
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-ornithine
anaerobic
34265
Clostridium sporogenes
L-proline + NH3
-
34265
Clostridium sporogenes
-
L-ornithine
-
34265
Clostridium sporogenes
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 41500, SDS-PAGE
Clostridium sporogenes
Other publictions for EC 4.3.1.12
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
729505
Sharma
Functional characterization of ...
Arabidopsis thaliana
BMC Plant Biol.
13
182
2013
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1
2
2
1
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-
730331
Jensen
Ornithine cyclodeaminase-based ...
Pseudomonas putida
Microb. Cell Fact.
12
63
2013
-
1
1
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-
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3
-
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729853
Ion
A molecular dynamics (MD) and ...
Pseudomonas putida
Int. J. Mol. Sci.
13
12994-13011
2012
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705095
Lee
Enhancement of ornithine produ ...
Corynebacterium glutamicum
J. Microbiol. Biotechnol.
20
127-131
2010
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2
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704292
Fonknechten
A conserved gene cluster rules ...
Acetoanaerobium sticklandii
J. Bacteriol.
191
3162-3167
2009
-
-
-
-
-
-
-
-
-
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-
1
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1
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1
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1
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1
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1
1
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-
-
693873
Cuervo
Differential soluble protein e ...
Trichomonas vaginalis
J. Proteomics
71
109-122
2008
-
-
-
-
-
-
-
-
-
-
1
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-
2
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1
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1
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1
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1
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1
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695039
Ozyurt
Computational active site anal ...
Saccharolobus solfataricus
Proteins
72
184-196
2008
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1
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1
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1
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1
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-
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-
-
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-
660605
Alam
Crystallization and X-ray diff ...
Pseudomonas putida
Acta Crystallogr. Sect. D
60
941-944
2004
-
-
1
1
-
-
-
-
-
-
-
-
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2
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1
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1
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1
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1
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661097
Goodman
Ornithine cyclodeaminase: stru ...
Pseudomonas putida
Biochemistry
43
13883-13891
2004
-
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1
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-
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-
-
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1
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1
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-
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-
34272
Soto
-
Ornithine cyclodeaminase activ ...
Agrobacterium tumefaciens, Rhodobacter sp., Sinorhizobium meliloti, Sinorhizobium meliloti 2011, Sinorhizobium meliloti 41, Sinorhizobium meliloti GR4
FEMS Microbiol. Lett.
11
209-214
1994
1
-
-
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-
1
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-
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11
-
7
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2
1
17
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2
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1
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2
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1
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11
-
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2
1
17
-
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34273
Soto
Identification of a novel Rhiz ...
Agrobacterium tumefaciens, Sinorhizobium meliloti, Sinorhizobium meliloti GR4
Mol. Plant Microbe Interact.
7
703-707
1994
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6
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10
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9
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6
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9
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34271
Schindler
Ornithine cyclodeaminase from ...
Agrobacterium tumefaciens
J. Bacteriol.
171
847-854
1989
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1
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3
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2
1
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5
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1
1
2
1
3
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2
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1
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1
1
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3
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2
1
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1
1
2
1
3
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2
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-
34269
Sans
Ornithine cyclodeaminase from ...
Agrobacterium tumefaciens
Eur. J. Biochem.
173
123-130
1988
2
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1
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3
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1
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6
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2
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2
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2
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1
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3
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1
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2
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2
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2
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34268
Stalon
Catabolism of arginine, citrul ...
Acetoanaerobium sticklandii, Burkholderia cepacia, Pseudomonas putida
J. Gen. Microbiol.
133
2487-2495
1987
-
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3
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7
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34270
Sans
The Noc region of Ti plasmid C ...
Agrobacterium tumefaciens
Eur. J. Biochem.
167
81-87
1987
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1
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34267
Dessaux
Arginine catabolism in Agrobac ...
Agrobacterium tumefaciens, Agrobacterium tumefaciens GV4R10c21, Agrobacterium tumefaciens R10, Clostridium sporogenes, Treponema denticola
J. Bacteriol.
166
44-50
1986
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1
1
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7
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10
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5
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10
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1
1
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34265
Muth
Ornithine cyclase (deaminating ...
Clostridium sporogenes
J. Biol. Chem.
249
7457-7462
1974
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1
4
2
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3
1
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1
2
-
1
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2
2
2
1
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3
-
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3
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1
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4
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2
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3
1
-
2
-
1
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2
2
2
1
-
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-
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-
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-
-
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34266
Muth
Ornithine cyclase (deaminating ...
Clostridium botulinum, Clostridium sporogenes
J. Biol. Chem.
249
7463-7467
1974
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-
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-
-
-
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2
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2
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1
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4
-
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1
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1
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-
-
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2
-
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-
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-
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4
-
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34264
Costilow
Ornithine cyclase (deaminating ...
Clostridium sporogenes
J. Biol. Chem.
246
6655-6660
1971
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1
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3
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1
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1
1
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1
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1
3
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1
1
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1
1
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1
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1
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1
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3
-
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1
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1
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1
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1
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1
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1
1
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