T-DNA mutants of atocd and AtOCD RNAi plants have 15% higher proline accumulation at low water potential while p5cs1-4/atocd double mutants have 40% higher proline than p5cs1 at low water potential but no change in proline metabolism gene expression
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hanging-drop vapor diffusion method, crystal structure of the enzyme in complex with NADH, refined to 1.8 A resolution, crystal structure of crystals grown in presence of L-ornithine, refined to 1.6 A resolution
vapor-diffusion method, the best diffraction-quality crystals are obtained from solutions of 40%(v/v) 2-methyl-2,4-pentanediol buffered at pH 6.0 with 0.1 M MES and diffracted X-rays to 1.68 A resolution. Crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 70.0, b = 78.3, c = 119.4 A